Restriction map for
pF1KB8728
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvrII BalI
BamHI BbeI BcgI BciVI BclI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI CelII ClaI CpoI
DraI DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FauI FbaI FseI HaeII
HindIII HpaI KasI KpnI KspI
MaeI MaeIII MamI MfeI MluI
MluNI MscI MspA1I MunI MvnI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 968 TCCGGA T^CCGGA
AciI 354 GCGG CCGC(-3/-1)
AcsI 786 GAATTC [GA]^AATT[CT]
AcyI 631 GACGCC G[GA]^CG[CT]C
Alw44I 472 GTGCAC G^TGCAC
AocI 21 CCTGAGG CC^T[ACGT]AGG
ApaLI 472 GTGCAC G^TGCAC
ApoI 786 GAATTC [GA]^AATT[CT]
AspHI 472 GTGCAC G[AT]GC[AT]^C
AvaII 456 GGACC G^G[AT]CC
AviII 924 TGCGCA TGC^GCA
BanI 261 GGTGCC G^G[CT][GA]CC
BanII 16 GGGCTC G[GA]GC[CT]^C
BbrPI 928 CACGTG CAC^GTG
BbvCI 880 GCTGAGG CCTCAGC(-5/-2)
BsaAI 928 CACGTG [CT]AC^GT[GA]
BsaHI 631 GACGCC G[GA]^CG[CT]C
BseAI 968 TCCGGA T^CCGGA
BsgI 637 CTGCAC GTGCAG(16/14)
BsiHKAI 472 GTGCAC G[AT]GC[AT]^C
BsmAI 486 GAGAC GTCTC(1/5)
BsmI 972 GAATGC GAATGC(1/-1)
BspEI 968 TCCGGA T^CCGGA
BspMI 187 ACCTGC ACCTGC(4/8)
BsrFI 338 GCCGGC [GA]^CCGG[CT]
Bst1107I 501 GTATAC GTA^TAC
BstZ17I 501 GTATAC GTA^TAC
Bsu36I 21 CCTGAGG CC^T[ACGT]AGG
CfoI 925 GCGC GCG^C
Cfr10I 338 GCCGGC [GA]^CCGG[CT]
DraIII 475 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 21 CCTGAGG CC^T[ACGT]AGG
EcoNI 447 CCTGATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 786 GAATTC G^AATTC
EcoT14I 545 CCATGG C^C[AT][AT]GG
FspI 924 TGCGCA TGC^GCA
HhaI 925 GCGC GCG^C
Hin1I 631 GACGCC G[GA]^CG[CT]C
HinP1I 925 GCGC G^CGC
HincII 535 GTTGAC GT[CT]^[GA]AC
HindII 535 GTTGAC GT[CT]^[GA]AC
MroI 968 TCCGGA T^CCGGA
NaeI 338 GCCGGC GCC^GGC
NcoI 545 CCATGG C^CATGG
NgoMIV 338 GCCGGC G^CCGGC
NspI 569 GCATGC [GA]CATG^[CT]
PmaCI 928 CACGTG CAC^GTG
PmlI 928 CACGTG CAC^GTG
PshAI 943 GACTCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SphI 569 GCATGC GCATG^C
SspI 1012 AATATT AAT^ATT
StyI 545 CCATGG C^C[AT][AT]GG
Tsp509I 787 AATT ^AATT
XcmI 1010 CCAATATTTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 501 GTATAC GT^[AC][GT]AC
961 GTCTAC
BcnI 66 CCCGG CC^[GC]GG
510 CCGGG
BglII 72 AGATCT A^GATCT
803 AGATCT
BsaWI 946 TCCGGT [AT]^CCGG[AT]
968 TCCGGA
BseRI 285 GAGGAG GAGGAG(10/8)
883 GAGGAG
DraII 136 AGGGCCT [GA]G^G[ACGT]CC[CT]
494 AGGCCCT
DsaI 545 CCATGG C^C[GA][CT]GG
711 CCGTGG
EarI 108 GAAGAG CTCTTC(1/4)
249 GAAGAG
EcoO109I 136 AGGGCCT [GA]G^G[ACGT]CC[CT]
494 AGGCCCT
Ksp632I 108 GAAGAG CTCTTC(1/4)
249 GAAGAG
MaeII 392 ACGT A^CGT
929 ACGT
MseI 197 TTAA T^TAA
452 TTAA
NciI 66 CCCGG CC^[GC]GG
510 CCGGG
PflMI 134 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
828 CCAGAGCTTGG
PstI 379 CTGCAG CTGCA^G
490 CTGCAG
SfcI 379 CTGCAG C^T[GA][CT]AG
490 CTGCAG
TfiI 207 GATTC G^A[AT]TC
773 GATTC
Tru9I 197 TTAA T^TAA
452 TTAA
Van91I 134 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
828 CCAGAGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AfaI AluI AlwI AlwNI BbsI
BbvI BmyI BpmI BpuAI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
TseI TspRI XhoII