Restriction map for
pF1KB8729
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# NO CUT ENZYMES ( 69 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AocI AscI AseI AsnI Asp700I
Asp718I AvrII BfrI BlnI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrGI BssHII BstBI Bsu36I ClaI
CpoI DraI Eco81I EcoRI EcoRV
EcoT22I FseI HindIII HpaI KpnI
MfeI MluI MroI MunI NdeI
NheI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SbfI SfuI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 1807 GAATTT [GA]^AATT[CT]
AflIII 866 ACATGT A^C[GA][CT]GT
Alw44I 1180 GTGCAC G^TGCAC
Aor51HI 2462 AGCGCT AGC^GCT
ApaLI 1180 GTGCAC G^TGCAC
ApoI 1807 GAATTT [GA]^AATT[CT]
AspI 1777 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 1210 GTCTTC GAAGAC(2/6)
BcgI 2235 GCAGCCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2040 GGATAC GTATCC(6/5)
BclI 1787 TGATCA T^GATCA
BfaI 373 CTAG C^TAG
BlpI 1353 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1353 GCTGAGC GC^T[ACGT]AGC
BpuAI 1210 GTCTTC GAAGAC(2/6)
BsaBI 183 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1525 GAGGAG GAGGAG(10/8)
BspHI 1784 TCATGA T^CATGA
BspLU11I 866 ACATGT A^CATGT
Bst1107I 2025 GTATAC GTA^TAC
BstXI 1034 CCAACGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2025 GTATAC GTA^TAC
CelII 1353 GCTGAGC GC^T[ACGT]AGC
DrdI 1162 GACGGCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2462 AGCGCT AGC^GCT
FbaI 1787 TGATCA T^GATCA
HincII 1768 GTCGAC GT[CT]^[GA]AC
HindII 1768 GTCGAC GT[CT]^[GA]AC
MaeI 373 CTAG C^TAG
MamI 183 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1291 TTAA T^TAA
NotI 629 GCGGCCGC GC^GGCCGC
NruI 2100 TCGCGA TCG^CGA
PflFI 1777 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1784 TCATGA T^CATGA
SacI 357 GAGCTC GAGCT^C
SalI 1768 GTCGAC G^TCGAC
ScaI 1436 AGTACT AGT^ACT
SexAI 2395 ACCAGGT A^CC[AT]GGT
SfiI 1581 GGCCATGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 660 CACCGGCG C[GA]^CCGG[CT]G
SmaI 860 CCCGGG CCC^GGG
SphI 1958 GCATGC GCATG^C
Tru9I 1291 TTAA T^TAA
Tth111I 1777 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 860 CCCGGG C^CCGGG
XmaI 860 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccI 1768 GTCGAC GT^[AC][GT]AC
2025 GTATAC
AhdI 1045 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2050 GACAGCATGTC
AspEI 1045 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2050 GACAGCATGTC
AviII 152 TGCGCA TGC^GCA
932 TGCGCA
BamHI 1019 GGATCC G^GATCC
1574 GGATCC
BbeI 451 GGCGCC GGCGC^C
2262 GGCGCC
BbrPI 436 CACGTG CAC^GTG
1480 CACGTG
BbvCI 2511 CCTCAGC CCTCAGC(-5/-2)
1914 GCTGAGG
BglI 582 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1582 GCCATGAAGGC
BglII 971 AGATCT A^GATCT
1745 AGATCT
BsaAI 436 CACGTG [CT]AC^GT[GA]
1480 CACGTG
BsmI 2146 GAATGC GAATGC(1/-1)
2380 GAATGC
BssSI 241 CACGAG CACGAG(-5/-1)
1522 CACGAG
DraIII 1174 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
2394 CACCAGGTG
EagI 630 CGGCCG C^GGCCG
1055 CGGCCG
Eam1105I 1045 GACCGTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2050 GACAGCATGTC
EclXI 630 CGGCCG C^GGCCG
1055 CGGCCG
Eco52I 630 CGGCCG C^GGCCG
1055 CGGCCG
FspI 152 TGCGCA TGC^GCA
932 TGCGCA
HinfI 482 GAGTC G^A[ACGT]TC
944 GACTC
KasI 451 GGCGCC G^GCGCC
2262 GGCGCC
KspI 1223 CCGCGG CCGC^GG
1271 CCGCGG
NaeI 620 GCCGGC GCC^GGC
1248 GCCGGC
NarI 451 GGCGCC GG^CGCC
2262 GGCGCC
NgoMIV 620 GCCGGC G^CCGGC
1248 GCCGGC
PleI 944 GACTC GAGTC(4/5)
482 GAGTC
PmaCI 436 CACGTG CAC^GTG
1480 CACGTG
PmlI 436 CACGTG CAC^GTG
1480 CACGTG
PvuII 2010 CAGCTG CAG^CTG
2167 CAGCTG
SacII 1223 CCGCGG CCGC^GG
1271 CCGCGG
SfoI 451 GGCGCC GGC^GCC
2262 GGCGCC
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsgI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoO65I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PpuMI PstI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII