Restriction map for
pF1KB8731
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BciVI BcnI BfrI
BglI BglII BlnI BpuAI BsaAI
BsaHI BsaI BseAI BsgI BsiEI
BsiWI BsiYI BslI BsmBI BsoBI
BspDI BspEI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI Fnu4HI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI ItaI KasI KpnI
KspI MaeII MfeI MluI MluNI
MroI MscI MspA1I MunI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI TseI Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 1113 ACATGT A^C[GA][CT]GT
Alw44I 1783 GTGCAC G^TGCAC
ApaLI 1783 GTGCAC G^TGCAC
AspI 3 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 597 GGACC G^G[AT]CC
BamHI 75 GGATCC G^GATCC
BclI 858 TGATCA T^GATCA
BlpI 1181 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1181 GCTCAGC GC^T[ACGT]AGC
BsaBI 256 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 98 ACCGGA [AT]^CCGG[AT]
BseRI 58 GAGGAG GAGGAG(10/8)
BsmFI 9 GTCCC GGGAC(10/14)
Bsp1407I 1194 TGTACA T^GTACA
BspLU11I 1113 ACATGT A^CATGT
BsrGI 1194 TGTACA T^GTACA
BstAPI 1015 GCATCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 75 GGATCC [GA]^GATC[CT]
CelII 1181 GCTCAGC GC^T[ACGT]AGC
Cfr13I 597 GGACC G^G[ACGT]CC
DsaI 196 CCATGG C^C[GA][CT]GG
EcoT14I 196 CCATGG C^C[AT][AT]GG
FbaI 858 TGATCA T^GATCA
HaeIII 1795 GGCC GG^CC
HapII 99 CCGG C^CGG
HinfI 7 GAGTC G^A[ACGT]TC
HpaII 99 CCGG C^CGG
MamI 256 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 75 GGATCC [GA]^GATC[CT]
MspI 99 CCGG C^CGG
MwoI 1015 GCATCCCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 196 CCATGG C^CATGG
NlaIV 75 GGATCC GG[ACGT]^[ACGT]CC
PflFI 3 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 7 GAGTC GAGTC(4/5)
SapI 1024 GCTCTTC GCTCTTC(1/4)
Sau96I 597 GGACC G^G[ACGT]CC
SspBI 1194 TGTACA T^GTACA
StyI 196 CCATGG C^C[AT][AT]GG
Tth111I 3 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 75 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 96 GTAC GT^AC
1195 GTAC
AlwI 76 GATCC GGATC(4/5)
75 GGATC
AspHI 1683 GTGCTC G[AT]GC[AT]^C
1783 GTGCAC
BfaI 112 CTAG C^TAG
1298 CTAG
BmyI 1683 GTGCTC G[AGT]GC[ACT]^C
1783 GTGCAC
BsiHKAI 1683 GTGCTC G[AT]GC[AT]^C
1783 GTGCAC
BsmAI 225 GAGAC GTCTC(1/5)
288 GAGAC
Bsp1286I 1683 GTGCTC G[AGT]GC[ACT]^C
1783 GTGCAC
BspHI 861 TCATGA T^CATGA
1449 TCATGA
BssKI 12 CCAGG ^CC[ACGT]GG
273 CCAGG
BstNI 12 CCAGG CC^[AT]GG
273 CCAGG
DpnII 76 GATC ^GATC
859 GATC
EarI 1025 CTCTTC CTCTTC(1/4)
66 GAAGAG
EcoRII 12 CCAGG ^CC[AT]GG
273 CCAGG
EcoT22I 809 ATGCAT ATGCA^T
977 ATGCAT
HindIII 746 AAGCTT A^AGCTT
1334 AAGCTT
Ksp632I 1025 CTCTTC CTCTTC(1/4)
66 GAAGAG
MaeI 112 CTAG C^TAG
1298 CTAG
MboI 76 GATC ^GATC
859 GATC
MvaI 12 CCAGG CC^[AT]GG
273 CCAGG
NdeII 76 GATC ^GATC
859 GATC
NsiI 809 ATGCAT ATGCA^T
977 ATGCAT
RcaI 861 TCATGA T^CATGA
1449 TCATGA
RsaI 96 GTAC GT^AC
1195 GTAC
Sau3AI 76 GATC ^GATC
859 GATC
ScrFI 12 CCAGG CC^[ACGT]GG
273 CCAGG
SfcI 654 CTGTAG C^T[GA][CT]AG
1905 CTGTAG
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AluI ApoI Asp700I BpmI
BsaJI BsmI BsrDI BsrI BstF5I
DdeI EcoRI FokI HphI MaeIII
MboII MnlI MseI MslI NdeI
NlaIII NspI SfaNI TaqI Tru9I
Tsp45I Tsp509I TspRI TthHB8I XmnI