Restriction map for
pF1KB8737
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiWI BsmAI BsmBI BsmFI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I MaeI MaeII
MamI MfeI MluI MroI MseI
MslI MunI NciI NcoI NdeI
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfaNI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI Tru9I Tsp509I
Tth111I TthHB8I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AlwI 186 GATCC GGATC(4/5)
AscI 139 GGCGCGCC GG^CGCGCC
AspHI 277 GAGCTC G[AT]GC[AT]^C
AvaI 390 CTCGGG C^[CT]CG[GA]G
BalI 257 TGGCCA TGG^CCA
BanII 277 GAGCTC G[GA]GC[CT]^C
BglI 406 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 277 GAGCTC G[AGT]GC[ACT]^C
BseRI 274 GAGGAG GAGGAG(10/8)
BsiEI 305 CGATCG CG[GA][CT]^CG
BsiHKAI 277 GAGCTC G[AT]GC[AT]^C
BsoBI 390 CTCGGG C^[CT]CG[GA]G
Bsp1286I 277 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 90 TGTACA T^GTACA
BspMI 248 ACCTGC ACCTGC(4/8)
BsrDI 203 GCAATG GCAATG(2/0)
BsrGI 90 TGTACA T^GTACA
BstYI 185 AGATCC [GA]^GATC[CT]
DdeI 182 CTGAG C^T[ACGT]AG
EaeI 257 TGGCCA [CT]^GGCC[GA]
EcoT14I 38 CCAAGG C^C[AT][AT]GG
HinfI 233 GAATC G^A[ACGT]TC
HphI 176 TCACC GGTGA(8/7)
KspI 9 CCGCGG CCGC^GG
MaeIII 301 GTGAC ^GT[ACGT]AC
MflI 185 AGATCC [GA]^GATC[CT]
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
PstI 250 CTGCAG CTGCA^G
PvuI 305 CGATCG CGAT^CG
PvuII 253 CAGCTG CAG^CTG
SacI 277 GAGCTC GAGCT^C
SacII 9 CCGCGG CCGC^GG
SfcI 250 CTGCAG C^T[GA][CT]AG
SfiI 405 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 135 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 90 TGTACA T^GTACA
StuI 413 AGGCCT AGG^CCT
StyI 38 CCAAGG C^C[AT][AT]GG
TfiI 233 GAATC G^A[AT]TC
Tsp45I 301 GTGAC ^GT[GC]AC
XcmI 362 CCAGCGCCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 185 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BanI 133 GGCGCC G^G[CT][GA]CC
384 GGCGCC
BbeI 133 GGCGCC GGCGC^C
384 GGCGCC
BbsI 225 GAAGAC GAAGAC(2/6)
357 GAAGAC
BpuAI 225 GAAGAC GAAGAC(2/6)
357 GAAGAC
BssHII 60 GCGCGC G^CGCGC
140 GCGCGC
DpnII 186 GATC ^GATC
306 GATC
DsaI 9 CCGCGG C^C[GA][CT]GG
371 CCGTGG
HgaI 228 GACGC GACGC(5/10)
320 GCGTC
KasI 133 GGCGCC G^GCGCC
384 GGCGCC
MboI 186 GATC ^GATC
306 GATC
MboII 225 GAAGA GAAGA(8/7)
357 GAAGA
NaeI 64 GCCGGC GCC^GGC
136 GCCGGC
NarI 133 GGCGCC GG^CGCC
384 GGCGCC
NdeII 186 GATC ^GATC
306 GATC
NgoMIV 64 GCCGGC G^CCGGC
136 GCCGGC
Sau3AI 186 GATC ^GATC
306 GATC
SfoI 133 GGCGCC GGC^GCC
384 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BbvI BpmI BsaHI BsaJI BsiYI
BslI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI MnlI MspA1I
MspI MvaI MvnI MwoI NlaIV
RsaI Sau96I ScrFI TseI TspRI