################################## # NO CUT ENZYMES ( 201 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacII SalI SapI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI StuI StyI SwaI TfiI Tru9I Tsp45I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AciI 63 CCGC CCGC(-3/-1) AcsI 316 GAATTT [GA]^AATT[CT] ApoI 316 GAATTT [GA]^AATT[CT] AspHI 286 GAGCTC G[AT]GC[AT]^C BanII 286 GAGCTC G[GA]GC[CT]^C BbsI 58 GAAGAC GAAGAC(2/6) BciVI 272 GTATCC GTATCC(6/5) BmyI 286 GAGCTC G[AGT]GC[ACT]^C BpuAI 58 GAAGAC GAAGAC(2/6) BsiHKAI 286 GAGCTC G[AT]GC[AT]^C BsmFI 111 GGGAC GGGAC(10/14) Bsp1286I 286 GAGCTC G[AGT]GC[ACT]^C BspHI 309 TCATGA T^CATGA BssKI 15 CCAGG ^CC[ACGT]GG BstAPI 36 GCAGCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 176 GGATG GGATG(2/0) BstNI 15 CCAGG CC^[AT]GG Cfr13I 146 GGGCC G^G[ACGT]CC DdeI 241 CTTAG C^T[ACGT]AG DpnII 165 GATC ^GATC EarI 25 GAAGAG CTCTTC(1/4) EcoNI 67 CCTTTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 15 CCAGG ^CC[AT]GG FokI 176 GGATG GGATG(9/13) Ksp632I 25 GAAGAG CTCTTC(1/4) MboI 165 GATC ^GATC MslI 307 CATCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 15 CCAGG CC^[AT]GG MwoI 36 GCAGCCTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeII 165 GATC ^GATC RcaI 309 TCATGA T^CATGA SacI 286 GAGCTC GAGCT^C Sau3AI 165 GATC ^GATC Sau96I 146 GGGCC G^G[ACGT]CC SbfI 94 CCTGCAGG CCTGCA^GG ScrFI 15 CCAGG CC^[ACGT]GG Sse8387I 94 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AluI 248 AGCT AG^CT 287 AGCT BbvI 36 GCAGC GCAGC(8/12) 249 GCTGC BseRI 74 GAGGAG GAGGAG(10/8) 214 GAGGAG Cac8I 93 GCCTGC GC[ACGT]^[ACGT]GC 118 GCTCGC Fnu4HI 36 GCAGC GC^[ACGT]GC 249 GCTGC HaeIII 85 GGCC GG^CC 147 GGCC ItaI 36 GCAGC GC^[ACGT]GC 249 GCTGC PstI 95 CTGCAG CTGCA^G 250 CTGCAG SfcI 95 CTGCAG C^T[GA][CT]AG 250 CTGCAG SspI 7 AATATT AAT^ATT 204 AATATT TaqI 105 TCGA T^CGA 141 TCGA TseI 36 GCAGC G^C[AT]GC 249 GCTGC TthHB8I 105 TCGA T^CGA 141 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### BsiYI BslI HphI MboII MnlI NlaIII Tsp509I