##################################
# NO CUT ENZYMES ( 201 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaI
HpaII KasI KpnI KspI MaeI
MaeII MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsaI RsrII SacII
SalI SapI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI SspBI
StuI StyI SwaI TfiI Tru9I
Tsp45I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AciI 63 CCGC CCGC(-3/-1)
AcsI 316 GAATTT [GA]^AATT[CT]
ApoI 316 GAATTT [GA]^AATT[CT]
AspHI 286 GAGCTC G[AT]GC[AT]^C
BanII 286 GAGCTC G[GA]GC[CT]^C
BbsI 58 GAAGAC GAAGAC(2/6)
BciVI 272 GTATCC GTATCC(6/5)
BmyI 286 GAGCTC G[AGT]GC[ACT]^C
BpuAI 58 GAAGAC GAAGAC(2/6)
BsiHKAI 286 GAGCTC G[AT]GC[AT]^C
BsmFI 111 GGGAC GGGAC(10/14)
Bsp1286I 286 GAGCTC G[AGT]GC[ACT]^C
BspHI 309 TCATGA T^CATGA
BssKI 15 CCAGG ^CC[ACGT]GG
BstAPI 36 GCAGCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 176 GGATG GGATG(2/0)
BstNI 15 CCAGG CC^[AT]GG
Cfr13I 146 GGGCC G^G[ACGT]CC
DdeI 241 CTTAG C^T[ACGT]AG
DpnII 165 GATC ^GATC
EarI 25 GAAGAG CTCTTC(1/4)
EcoNI 67 CCTTTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 15 CCAGG ^CC[AT]GG
FokI 176 GGATG GGATG(9/13)
Ksp632I 25 GAAGAG CTCTTC(1/4)
MboI 165 GATC ^GATC
MslI 307 CATCATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 15 CCAGG CC^[AT]GG
MwoI 36 GCAGCCTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII 165 GATC ^GATC
RcaI 309 TCATGA T^CATGA
SacI 286 GAGCTC GAGCT^C
Sau3AI 165 GATC ^GATC
Sau96I 146 GGGCC G^G[ACGT]CC
SbfI 94 CCTGCAGG CCTGCA^GG
ScrFI 15 CCAGG CC^[ACGT]GG
Sse8387I 94 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AluI 248 AGCT AG^CT
287 AGCT
BbvI 36 GCAGC GCAGC(8/12)
249 GCTGC
BseRI 74 GAGGAG GAGGAG(10/8)
214 GAGGAG
Cac8I 93 GCCTGC GC[ACGT]^[ACGT]GC
118 GCTCGC
Fnu4HI 36 GCAGC GC^[ACGT]GC
249 GCTGC
HaeIII 85 GGCC GG^CC
147 GGCC
ItaI 36 GCAGC GC^[ACGT]GC
249 GCTGC
PstI 95 CTGCAG CTGCA^G
250 CTGCAG
SfcI 95 CTGCAG C^T[GA][CT]AG
250 CTGCAG
SspI 7 AATATT AAT^ATT
204 AATATT
TaqI 105 TCGA T^CGA
141 TCGA
TseI 36 GCAGC G^C[AT]GC
249 GCTGC
TthHB8I 105 TCGA T^CGA
141 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 7 enzymes) #
#########################################
BsiYI BslI HphI MboII MnlI
NlaIII Tsp509I