Restriction map for pF1KB8738
##################################
# NO CUT ENZYMES (  201 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HpaII       KasI        KpnI        KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsaI        RsrII       SacII       
SalI        SapI        ScaI        SexAI       SfaNI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        SspBI       
StuI        StyI        SwaI        TfiI        Tru9I       
Tsp45I      TspRI       Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AciI           63  CCGC                 CCGC(-3/-1)
AcsI          316  GAATTT               [GA]^AATT[CT]
ApoI          316  GAATTT               [GA]^AATT[CT]
AspHI         286  GAGCTC               G[AT]GC[AT]^C
BanII         286  GAGCTC               G[GA]GC[CT]^C
BbsI           58  GAAGAC               GAAGAC(2/6)
BciVI         272  GTATCC               GTATCC(6/5)
BmyI          286  GAGCTC               G[AGT]GC[ACT]^C
BpuAI          58  GAAGAC               GAAGAC(2/6)
BsiHKAI       286  GAGCTC               G[AT]GC[AT]^C
BsmFI         111  GGGAC                GGGAC(10/14)
Bsp1286I      286  GAGCTC               G[AGT]GC[ACT]^C
BspHI         309  TCATGA               T^CATGA
BssKI          15  CCAGG                ^CC[ACGT]GG
BstAPI         36  GCAGCCTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        176  GGATG                GGATG(2/0)
BstNI          15  CCAGG                CC^[AT]GG
Cfr13I        146  GGGCC                G^G[ACGT]CC
DdeI          241  CTTAG                C^T[ACGT]AG
DpnII         165  GATC                 ^GATC
EarI           25  GAAGAG               CTCTTC(1/4)
EcoNI          67  CCTTTGGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII         15  CCAGG                ^CC[AT]GG
FokI          176  GGATG                GGATG(9/13)
Ksp632I        25  GAAGAG               CTCTTC(1/4)
MboI          165  GATC                 ^GATC
MslI          307  CATCATGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI           15  CCAGG                CC^[AT]GG
MwoI           36  GCAGCCTATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII         165  GATC                 ^GATC
RcaI          309  TCATGA               T^CATGA
SacI          286  GAGCTC               GAGCT^C
Sau3AI        165  GATC                 ^GATC
Sau96I        146  GGGCC                G^G[ACGT]CC
SbfI           94  CCTGCAGG             CCTGCA^GG
ScrFI          15  CCAGG                CC^[ACGT]GG
Sse8387I       94  CCTGCAGG             CCTGCA^GG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AluI          248  AGCT                 AG^CT
              287  AGCT                
BbvI           36  GCAGC                GCAGC(8/12)
              249  GCTGC               
BseRI          74  GAGGAG               GAGGAG(10/8)
              214  GAGGAG              
Cac8I          93  GCCTGC               GC[ACGT]^[ACGT]GC
              118  GCTCGC              
Fnu4HI         36  GCAGC                GC^[ACGT]GC
              249  GCTGC               
HaeIII         85  GGCC                 GG^CC
              147  GGCC                
ItaI           36  GCAGC                GC^[ACGT]GC
              249  GCTGC               
PstI           95  CTGCAG               CTGCA^G
              250  CTGCAG              
SfcI           95  CTGCAG               C^T[GA][CT]AG
              250  CTGCAG              
SspI            7  AATATT               AAT^ATT
              204  AATATT              
TaqI          105  TCGA                 T^CGA
              141  TCGA                
TseI           36  GCAGC                G^C[AT]GC
              249  GCTGC               
TthHB8I       105  TCGA                 T^CGA
              141  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BsiYI       BslI        HphI        MboII       MnlI        
NlaIII      Tsp509I     
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