##################################
# NO CUT ENZYMES (  232 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwI        AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BbvI        BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BmyI        BpmI        BpuAI       
BsaAI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmBI       BsmFI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FokI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HpaI        HpaII       HphI        ItaI        
KasI        KpnI        KspI        MaeI        MaeIII      
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MspI        MunI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NlaIV       NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SalI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TseI        
Tsp45I      TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

BlpI           26  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       26  GCTCAGC              GC^T[ACGT]AGC
BsaBI         132  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
CelII          26  GCTCAGC              GC^T[ACGT]AGC
DdeI           27  CTCAG                C^T[ACGT]AG
DpnII         132  GATC                 ^GATC
EarI           38  GAAGAG               CTCTTC(1/4)
HinfI          87  GAATC                G^A[ACGT]TC
Ksp632I        38  GAAGAG               CTCTTC(1/4)
MaeII          72  ACGT                 A^CGT
MamI          132  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI          132  GATC                 ^GATC
MboII          38  GAAGA                GAAGA(8/7)
MseI          115  TTAA                 T^TAA
NdeII         132  GATC                 ^GATC
SapI           38  GAAGAGC              GCTCTTC(1/4)
Sau3AI        132  GATC                 ^GATC
SfaNI         147  GATGC                GCATC(5/9)
SfcI           82  CTACAG               C^T[GA][CT]AG
TfiI           87  GAATC                G^A[AT]TC
Tru9I         115  TTAA                 T^TAA
Tsp509I       100  AATT                 ^AATT


###################################
# TWO CUT ENZYMES (    3 enzymes) #
###################################

AluI           30  AGCT                 AG^CT
               80  AGCT                
BsmAI         107  GTCTC                GTCTC(1/5)
              122  GTCTC               
MslI           51  CATTGAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              141  CATCGTGATG          


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MnlI