Restriction map for
pF1KB8740
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanII BbrPI BbvCI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspHI BsrBI
BsrDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DdeI
DraI DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeI MaeIII MflI MluI
MluNI MroI MscI MspA1I NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tsp45I Tth111I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 57 enzymes) #
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AfaI 261 GTAC GT^AC
AflIII 257 ACATGT A^C[GA][CT]GT
AlwNI 354 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 54 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 469 GTGCTC G[AT]GC[AT]^C
BbeI 67 GGCGCC GGCGC^C
BbsI 421 GAAGAC GAAGAC(2/6)
BpmI 187 CTGGAG CTGGAG(16/14)
BpuAI 421 GAAGAC GAAGAC(2/6)
BsaAI 271 TACGTG [CT]AC^GT[GA]
BsaBI 3 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 469 GTGCTC G[AT]GC[AT]^C
Bsp1407I 260 TGTACA T^GTACA
BspLU11I 257 ACATGT A^CATGT
BspMI 449 ACCTGC ACCTGC(4/8)
BsrGI 260 TGTACA T^GTACA
BsrI 205 CCAGT ACTGG(1/-1)
BstAPI 462 GCACACTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 525 GATC ^GATC
DraII 311 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 311 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 368 CCAAGG C^C[AT][AT]GG
FauI 455 GCGGG CCCGC(4/6)
HgaI 409 GACGC GACGC(5/10)
HphI 93 GGTGA GGTGA(8/7)
KasI 67 GGCGCC G^GCGCC
MamI 3 GATGCAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 525 GATC ^GATC
MfeI 537 CAATTG C^AATTG
MseI 44 TTAA T^TAA
MslI 375 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 537 CAATTG C^AATTG
NaeI 412 GCCGGC GCC^GGC
NarI 67 GGCGCC GG^CGCC
NdeII 525 GATC ^GATC
NgoMIV 412 GCCGGC G^CCGGC
NspI 257 ACATGT [GA]CATG^[CT]
PflMI 331 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 214 GACTC GAGTC(4/5)
PpuMI 311 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 261 GTAC GT^AC
Sau3AI 525 GATC ^GATC
SfaNI 3 GATGC GCATC(5/9)
SfoI 67 GGCGCC GGC^GCC
SgrAI 411 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 136 CCCGGG CCC^GGG
SmlI 280 CTCAAG C^T[CT][GA]AG
SspBI 260 TGTACA T^GTACA
StyI 368 CCAAGG C^C[AT][AT]GG
TfiI 54 GAATC G^A[AT]TC
Tru9I 44 TTAA T^TAA
Tsp509I 538 AATT ^AATT
Van91I 331 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 368 CCAAGGACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 136 CCCGGG C^CCGGG
XmaI 136 CCCGGG C^CCGGG
XmnI 54 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 67 GGCGCC G[GA]^CG[CT]C
409 GACGCC
AluI 106 AGCT AG^CT
235 AGCT
BanI 67 GGCGCC G^G[CT][GA]CC
99 GGTGCC
BcgI 233 GCAGCTTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
293 GCAACGACATCG
BcnI 136 CCCGG CC^[GC]GG
137 CCGGG
BsaHI 67 GGCGCC G[GA]^CG[CT]C
409 GACGCC
BseRI 190 GAGGAG GAGGAG(10/8)
346 GAGGAG
BsgI 316 CTGCAC GTGCAG(16/14)
460 CTGCAC
BsmFI 254 GGGAC GGGAC(10/14)
208 GTCCC
BsrFI 333 ACCGGC [GA]^CCGG[CT]
412 GCCGGC
Cfr10I 333 ACCGGC [GA]^CCGG[CT]
412 GCCGGC
DraIII 268 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
463 CACACTGTG
HaeII 67 GGCGCC [GA]GCGC^[CT]
223 AGCGCC
HaeIII 391 GGCC GG^CC
544 GGCC
Hin1I 67 GGCGCC G[GA]^CG[CT]C
409 GACGCC
HinfI 54 GAATC G^A[ACGT]TC
214 GACTC
MaeII 119 ACGT A^CGT
272 ACGT
MboII 418 GAAGA GAAGA(8/7)
421 GAAGA
NciI 136 CCCGG CC^[GC]GG
137 CCGGG
TaqI 302 TCGA T^CGA
475 TCGA
TthHB8I 302 TCGA T^CGA
475 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AvaI AvaII BbvI
BmyI BsaJI BsiYI BslI BsoBI
Bsp1286I BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I EcoRII Fnu4HI
FokI HapII HhaI HinP1I HpaII
ItaI MnlI MspI MvaI MvnI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI TspRI