Restriction map for
pF1KB8742
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspHI AspI AviII
AvrII BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaHI BseAI BsiEI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MroI MseI MspA1I MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmeI PpuMI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AcsI 706 AAATTC [GA]^AATT[CT]
AfaI 1062 GTAC GT^AC
AhdI 77 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1074 GGATC GGATC(4/5)
Aor51HI 871 AGCGCT AGC^GCT
ApoI 706 AAATTC [GA]^AATT[CT]
Asp700I 991 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 77 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1051 CTCGAG C^[CT]CG[GA]G
BalI 494 TGGCCA TGG^CCA
BbrPI 712 CACGTG CAC^GTG
BbvI 211 GCTGC GCAGC(8/12)
BcnI 289 CCCGG CC^[GC]GG
BglII 470 AGATCT A^GATCT
BsaAI 712 CACGTG [CT]AC^GT[GA]
BsaWI 481 ACCGGA [AT]^CCGG[AT]
BsmBI 778 GAGACG CGTCTC(1/5)
BsmFI 278 GGGAC GGGAC(10/14)
BsoBI 1051 CTCGAG C^[CT]CG[GA]G
BspHI 323 TCATGA T^CATGA
BspMI 149 ACCTGC ACCTGC(4/8)
BsrBI 676 GAGCGG CCGCTC(-3/-3)
BsrDI 1118 GCAATG GCAATG(2/0)
BstXI 630 CCAGCTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 470 AGATCT [GA]^GATC[CT]
CfoI 872 GCGC GCG^C
EaeI 494 TGGCCA [CT]^GGCC[GA]
Eam1105I 77 GACCCCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 609 GAAGAG CTCTTC(1/4)
Eco47III 871 AGCGCT AGC^GCT
EcoT14I 665 CCAAGG C^C[AT][AT]GG
Fnu4HI 211 GCTGC GC^[ACGT]GC
HaeII 871 AGCGCT [GA]GCGC^[CT]
HgaI 83 GCGTC GACGC(5/10)
HhaI 872 GCGC GCG^C
HinP1I 872 GCGC G^CGC
ItaI 211 GCTGC GC^[ACGT]GC
Ksp632I 609 GAAGAG CTCTTC(1/4)
MaeIII 661 GTCAC ^GT[ACGT]AC
MflI 470 AGATCT [GA]^GATC[CT]
MluNI 494 TGGCCA TGG^CCA
MscI 494 TGGCCA TGG^CCA
NciI 289 CCCGG CC^[GC]GG
NspI 20 GCATGT [GA]CATG^[CT]
PaeR7I 1051 CTCGAG C^TCGAG
PflMI 765 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 712 CACGTG CAC^GTG
PmlI 712 CACGTG CAC^GTG
PshAI 654 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 649 CTGCAG CTGCA^G
RcaI 323 TCATGA T^CATGA
RsaI 1062 GTAC GT^AC
SapI 609 GAAGAGC GCTCTTC(1/4)
SexAI 229 ACCTGGT A^CC[AT]GGT
SfcI 649 CTGCAG C^T[GA][CT]AG
StyI 665 CCAAGG C^C[AT][AT]GG
TseI 211 GCTGC G^C[AT]GC
Tsp45I 661 GTCAC ^GT[GC]AC
Van91I 765 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1051 CTCGAG C^TCGAG
XhoII 470 AGATCT [GA]^GATC[CT]
XmnI 991 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 82 CGCG CG^CG
264 CGCG
AlwNI 540 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1031 CAGATTCTG
AvaII 164 GGACC G^G[AT]CC
840 GGTCC
BsaBI 465 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
471 GATCTTCATC
BsaI 526 GAGACC GGTCTC(1/5)
794 GAGACC
BsgI 548 GTGCAG GTGCAG(16/14)
1028 GTGCAG
BsrI 343 ACTGG ACTGG(1/-1)
917 ACTGG
BstUI 82 CGCG CG^CG
264 CGCG
Cfr13I 164 GGACC G^G[ACGT]CC
840 GGTCC
DpnII 471 GATC ^GATC
1075 GATC
FauI 80 CCCGC CCCGC(4/6)
519 GCGGG
HaeIII 69 GGCC GG^CC
495 GGCC
HapII 290 CCGG C^CGG
482 CCGG
HpaII 290 CCGG C^CGG
482 CCGG
MamI 465 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
471 GATCTTCATC
MboI 471 GATC ^GATC
1075 GATC
MspI 290 CCGG C^CGG
482 CCGG
MvnI 82 CGCG CG^CG
264 CGCG
NdeII 471 GATC ^GATC
1075 GATC
NlaIV 233 GGTTCC GG[ACGT]^[ACGT]CC
484 GGAACC
Sau3AI 471 GATC ^GATC
1075 GATC
Sau96I 164 GGACC G^G[ACGT]CC
840 GGTCC
SfaNI 4 GATGC GCATC(5/9)
320 GCATC
SmlI 64 CTCAAG C^T[CT][GA]AG
1051 CTCGAG
TaqI 176 TCGA T^CGA
1052 TCGA
Tsp509I 117 AATT ^AATT
707 AATT
TthHB8I 176 TCGA T^CGA
1052 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI BanII BmyI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI Cac8I DdeI
EcoRII FokI HinfI HphI MaeII
MboII MnlI MslI MvaI MwoI
NlaIII PleI ScrFI TfiI TspRI
XcmI