##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
Alw44I      AlwI        AlwNI       AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspHI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiHKAI     BsiWI       
BsmAI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstNI       BstPI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DpnII       DraI        DraIII      
DrdI        EarI        Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FbaI        FokI        FseI        FspI        HincII      
HindII      HindIII     HinfI       HpaI        KasI        
KpnI        Ksp632I     MaeI        MamI        MboI        
MboII       MfeI        MflI        MluI        MluNI       
MroI        MscI        MseI        MslI        MunI        
MvaI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

AhdI          165  GACGATGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI          228  AGCT                 AG^CT
Aor51HI       236  AGCGCT               AGC^GCT
ApaI          285  GGGCCC               GGGCC^C
AspEI         165  GACGATGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI           10  GCCTCGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI         159  GAGGAG               GAGGAG(10/8)
BsrFI         344  GCCGGT               [GA]^CCGG[CT]
BssHII        314  GCGCGC               G^CGCGC
Cfr10I        344  GCCGGT               [GA]^CCGG[CT]
DdeI          303  CTCAG                C^T[ACGT]AG
EaeI           49  CGGCCG               [CT]^GGCC[GA]
EagI           49  CGGCCG               C^GGCCG
Eam1105I      165  GACGATGCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          49  CGGCCG               C^GGCCG
Eco47III      236  AGCGCT               AGC^GCT
Eco52I         49  CGGCCG               C^GGCCG
HphI          297  TCACC                GGTGA(8/7)
MaeII         119  ACGT                 A^CGT
NlaIII        323  CATG                 CATG^
NspI          322  GCATGC               [GA]CATG^[CT]
PpuMI         130  GGGACCC              [GA]G^G[AT]CC[CT]
SfcI          309  CTGTAG               C^T[GA][CT]AG
SphI          322  GCATGC               GCATG^C
TspRI          29  CACTG                CA[GC]TG[ACGT][ACGT]^
XcmI           20  CCACGGCATCACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AvaI           12  CTCGGG               C^[CT]CG[GA]G
              232  CCCGAG              
AvaII         131  GGACC                G^G[AT]CC
              211  GGTCC               
BanI          341  GGTGCC               G^G[CT][GA]CC
              347  GGTGCC              
BanII          44  GAGCCC               G[GA]GC[CT]^C
              285  GGGCCC              
BsiEI          49  CGGCCG               CG[GA][CT]^CG
              357  CGGTCG              
BsoBI          12  CTCGGG               C^[CT]CG[GA]G
              232  CCCGAG              
BsrI           30  ACTGG                ACTGG(1/-1)
              113  CCAGT               
DraII         130  GGGACCC              [GA]G^G[ACGT]CC[CT]
              284  GGGGCCC             
EcoO109I      130  GGGACCC              [GA]G^G[ACGT]CC[CT]
              284  GGGGCCC             
HaeII         218  AGCGCC               [GA]GCGC^[CT]
              236  AGCGCT              
KspI           52  CCGCGG               CCGC^GG
              289  CCGCGG              
MaeIII        116  GTCAC                ^GT[ACGT]AC
              296  GTCAC               
SacII          52  CCGCGG               CCGC^GG
              289  CCGCGG              
SfaNI         168  GATGC                GCATC(5/9)
               25  GCATC               
Tsp45I        116  GTCAC                ^GT[GC]AC
              296  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   38 enzymes) #
#########################################

AccII       AciI        AcyI        BbvI        BcnI        
BmyI        BsaHI       BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BssKI       BstUI       Cac8I       
CfoI        Cfr13I      DsaI        FauI        Fnu4HI      
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       ItaI        MnlI        MspA1I      
MspI        MvnI        MwoI        NciI        NlaIV       
Sau96I      ScrFI       TseI