################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DpnII DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MboI MfeI MflI MluI MroI MseI MslI MunI NarI NdeI NdeII NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfaNI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 26 enzymes) # ################################### AcyI 420 GGCGTC G[GA]^CG[CT]C AfaI 71 GTAC GT^AC BalI 173 TGGCCA TGG^CCA BanII 14 GAGCCC G[GA]GC[CT]^C BpmI 298 CTGGAG CTGGAG(16/14) BsaHI 420 GGCGTC G[GA]^CG[CT]C BsmFI 449 GGGAC GGGAC(10/14) EarI 488 CTCTTC CTCTTC(1/4) EcoNI 321 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 456 GGCGCT [GA]GCGC^[CT] Hin1I 420 GGCGTC G[GA]^CG[CT]C HinfI 125 GACTC G^A[ACGT]TC HphI 278 TCACC GGTGA(8/7) Ksp632I 488 CTCTTC CTCTTC(1/4) MaeII 452 ACGT A^CGT MboII 489 TCTTC GAAGA(8/7) MluNI 173 TGGCCA TGG^CCA MscI 173 TGGCCA TGG^CCA PleI 125 GACTC GAGTC(4/5) RsaI 71 GTAC GT^AC SapI 487 GCTCTTC GCTCTTC(1/4) SfiI 364 GGCCCGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC TaqI 239 TCGA T^CGA TspRI 262 CACTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 239 TCGA T^CGA XcmI 515 CCACAGAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 79 CGCG CG^CG 474 CGCG AvaII 303 GGACC G^G[AT]CC 330 GGTCC BglI 142 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 365 GCCCGCTGGGC BmyI 14 GAGCCC G[AGT]GC[ACT]^C 196 GTGCCC Bsp1286I 14 GAGCCC G[AGT]GC[ACT]^C 196 GTGCCC BsrFI 147 GCCGGC [GA]^CCGG[CT] 374 GCCGGC BstUI 79 CGCG CG^CG 474 CGCG Cfr10I 147 GCCGGC [GA]^CCGG[CT] 374 GCCGGC DraII 317 AGGCCCT [GA]G^G[ACGT]CC[CT] 521 AGGCCCT EaeI 173 TGGCCA [CT]^GGCC[GA] 470 TGGCCG EcoO109I 317 AGGCCCT [GA]G^G[ACGT]CC[CT] 521 AGGCCCT HgaI 246 GACGC GACGC(5/10) 421 GCGTC MaeIII 244 GTGAC ^GT[ACGT]AC 266 GTGAC MvnI 79 CGCG CG^CG 474 CGCG NaeI 147 GCCGGC GCC^GGC 374 GCCGGC NcoI 92 CCATGG C^CATGG 360 CCATGG NgoMIV 147 GCCGGC G^CCGGC 374 GCCGGC SmaI 201 CCCGGG CCC^GGG 435 CCCGGG Tsp45I 244 GTGAC ^GT[GC]AC 266 GTGAC XmaCI 201 CCCGGG C^CCGGG 435 CCCGGG XmaI 201 CCCGGG C^CCGGG 435 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AluI AvaI BbvI BcnI BsaJI BsiYI BslI BsoBI BspMI BssKI BstNI Cac8I CfoI Cfr13I DsaI EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PstI PvuII Sau96I ScrFI SfcI StyI TseI