##################################
# NO CUT ENZYMES (  234 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      Cac8I       CelII       CfoI        Cfr10I      
ClaI        CpoI        DdeI        DpnII       DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRII      
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HinfI       HpaI        
HphI        ItaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MspA1I      MunI        MvaI        MvnI        MwoI        
NaeI        NarI        NcoI        NdeI        NdeII       
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfaNI       SfcI        
SfiI        SfoI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   20 enzymes) #
###################################

AfaI           90  GTAC                 GT^AC
AluI           20  AGCT                 AG^CT
AvaI           14  CCCGGG               C^[CT]CG[GA]G
AvaII          80  GGTCC                G^G[AT]CC
BsaJI          14  CCCGGG               C^C[ACGT][ACGT]GG
BsoBI          14  CCCGGG               C^[CT]CG[GA]G
Cfr13I         80  GGTCC                G^G[ACGT]CC
DraII          79  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoO109I       79  AGGTCCT              [GA]G^G[ACGT]CC[CT]
HapII          15  CCGG                 C^CGG
HpaII          15  CCGG                 C^CGG
MnlI           78  GAGG                 CCTC(7/6)
MspI           15  CCGG                 C^CGG
NlaIV          68  GGCTCC               GG[ACGT]^[ACGT]CC
PpuMI          79  AGGTCCT              [GA]G^G[AT]CC[CT]
RsaI           90  GTAC                 GT^AC
Sau96I         80  GGTCC                G^G[ACGT]CC
SmaI           14  CCCGGG               CCC^GGG
XmaCI          14  CCCGGG               C^CCGGG
XmaI           14  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (    4 enzymes) #
###################################

BcnI           14  CCCGG                CC^[GC]GG
               15  CCGGG               
BssKI          14  CCCGG                ^CC[ACGT]GG
               15  CCGGG               
NciI           14  CCCGG                CC^[GC]GG
               15  CCGGG               
ScrFI          14  CCCGG                CC^[ACGT]GG
               15  CCGGG               


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################