Restriction map for
pF1KB8749
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AfaI AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AvaII
AviII AvrII BamHI BbvCI BciVI
BclI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsiYI BslI BsmFI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstYI
BstZ17I Cac8I CelII Cfr10I ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI KpnI KspI MamI MflI
MluI MroI MvnI NaeI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmeI PpuMI PshBI
Psp1406I PstI PvuI RcaI RsaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Van91I
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 133 GTCTAC GT^[AC][GT]AC
AcsI 908 AAATTC [GA]^AATT[CT]
AcyI 209 GGCGCC G[GA]^CG[CT]C
AflII 465 CTTAAG C^TTAAG
AlwNI 1140 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1156 CCTGAGG CC^T[ACGT]AGG
ApoI 908 AAATTC [GA]^AATT[CT]
AspI 337 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 902 CCCGAG C^[CT]CG[GA]G
BalI 506 TGGCCA TGG^CCA
BanI 209 GGCGCC G^G[CT][GA]CC
BbeI 209 GGCGCC GGCGC^C
BbrPI 628 CACGTG CAC^GTG
BcgI 1078 CGAGAATCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 747 CCCGG CC^[GC]GG
BfrI 465 CTTAAG C^TTAAG
BglI 508 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 454 CTCCAG CTGGAG(16/14)
BsaAI 628 CACGTG [CT]AC^GT[GA]
BsaHI 209 GGCGCC G[GA]^CG[CT]C
BseRI 1159 GAGGAG GAGGAG(10/8)
BsmBI 1107 GAGACG CGTCTC(1/5)
BsoBI 902 CCCGAG C^[CT]CG[GA]G
BssSI 249 CTCGTG CACGAG(-5/-1)
BstEII 741 GGTCACC G^GT[ACGT]ACC
BstPI 741 GGTCACC G^GT[ACGT]ACC
Bsu36I 1156 CCTGAGG CC^T[ACGT]AGG
DraII 358 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eco81I 1156 CCTGAGG CC^T[ACGT]AGG
EcoO109I 358 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 741 GGTCACC G^GT[ACGT]ACC
FauI 60 CCCGC CCCGC(4/6)
HaeII 209 GGCGCC [GA]GCGC^[CT]
HapII 748 CCGG C^CGG
HgaI 40 GACGC GACGC(5/10)
Hin1I 209 GGCGCC G[GA]^CG[CT]C
HindIII 586 AAGCTT A^AGCTT
HpaII 748 CCGG C^CGG
KasI 209 GGCGCC G^GCGCC
MfeI 1027 CAATTG C^AATTG
MluNI 506 TGGCCA TGG^CCA
MscI 506 TGGCCA TGG^CCA
MspI 748 CCGG C^CGG
MunI 1027 CAATTG C^AATTG
NarI 209 GGCGCC GG^CGCC
NciI 747 CCCGG CC^[GC]GG
NdeI 214 CATATG CA^TATG
PflFI 337 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 628 CACGTG CAC^GTG
PmlI 628 CACGTG CAC^GTG
PshAI 1109 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 89 GAAGAGC GCTCTTC(1/4)
SfaNI 473 GCATC GCATC(5/9)
SfcI 1238 CTGTAG C^T[GA][CT]AG
SfoI 209 GGCGCC GGC^GCC
SmlI 465 CTTAAG C^T[CT][GA]AG
Tth111I 337 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 883 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AlwI 313 GATCC GGATC(4/5)
450 GATCC
AspHI 67 GAGCAC G[AT]GC[AT]^C
439 GTGCTC
BbsI 98 GAAGAC GAAGAC(2/6)
673 GAAGAC
BfaI 376 CTAG C^TAG
884 CTAG
BpuAI 98 GAAGAC GAAGAC(2/6)
673 GAAGAC
BsaI 379 GAGACC GGTCTC(1/5)
1162 GAGACC
BsgI 1086 CTGCAC GTGCAG(16/14)
1124 GTGCAG
BsiHKAI 67 GAGCAC G[AT]GC[AT]^C
439 GTGCTC
BstF5I 735 CATCC GGATG(2/0)
689 GGATG
BstNI 52 CCAGG CC^[AT]GG
738 CCTGG
BstXI 188 CCACTCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1022 CCAAGCAATTGG
CfoI 210 GCGC GCG^C
603 GCGC
Cfr13I 359 GGGCC G^G[ACGT]CC
918 GGGCC
DdeI 80 CTGAG C^T[ACGT]AG
1157 CTGAG
DraI 703 TTTAAA TTT^AAA
1224 TTTAAA
DraIII 201 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
326 CACCTTGTG
EarI 84 GAAGAG CTCTTC(1/4)
89 GAAGAG
EcoRII 52 CCAGG ^CC[AT]GG
738 CCTGG
EcoT14I 503 CCTTGG C^C[AT][AT]GG
1100 CCAAGG
FokI 735 CATCC GGATG(9/13)
689 GGATG
HhaI 210 GCGC GCG^C
603 GCGC
HinP1I 210 GCGC G^CGC
603 GCGC
Ksp632I 84 GAAGAG CTCTTC(1/4)
89 GAAGAG
MaeI 376 CTAG C^TAG
884 CTAG
MslI 112 CACAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
189 CACTCTTGTG
MspA1I 123 CAGCTG C[AC]G^C[GT]G
1143 CAGCTG
MvaI 52 CCAGG CC^[AT]GG
738 CCTGG
PvuII 123 CAGCTG CAG^CTG
1143 CAGCTG
Sau96I 359 GGGCC G^G[ACGT]CC
918 GGGCC
StyI 503 CCTTGG C^C[AT][AT]GG
1100 CCAAGG
TaqI 525 TCGA T^CGA
1077 TCGA
Tsp45I 742 GTCAC ^GT[GC]AC
838 GTGAC
TthHB8I 525 TCGA T^CGA
1077 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsmAI Bsp1286I BsrI BssKI
DpnII EaeI Fnu4HI HaeIII HincII
HindII HinfI HphI ItaI MaeII
MaeIII MboI MboII MnlI MseI
MwoI NdeII NlaIII NlaIV Sau3AI
ScrFI TfiI Tru9I TseI Tsp509I
TspRI