##################################
# NO CUT ENZYMES (  193 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      ClaI        
CpoI        DraI        DraII       DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspI        SwaI        
TaqI        Tru9I       Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AfaI          114  GTAC                 GT^AC
AluI           45  AGCT                 AG^CT
AvaII         270  GGTCC                G^G[AT]CC
BbvCI          49  GCTGAGG              CCTCAGC(-5/-2)
BbvI           46  GCTGC                GCAGC(8/12)
BmyI          120  GGGCAC               G[AGT]GC[ACT]^C
BseRI          52  GAGGAG               GAGGAG(10/8)
Bsp1286I      120  GGGCAC               G[AGT]GC[ACT]^C
Bsp1407I      113  TGTACA               T^GTACA
BspHI         240  TCATGA               T^CATGA
BsrDI         146  GCAATG               GCAATG(2/0)
BsrGI         113  TGTACA               T^GTACA
BsrI          410  ACTGG                ACTGG(1/-1)
BstF5I        339  CATCC                GGATG(2/0)
DdeI           50  CTGAG                C^T[ACGT]AG
DraIII        369  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I        80  CCATGG               C^C[AT][AT]GG
FauI          107  CCCGC                CCCGC(4/6)
FokI          339  CATCC                GGATG(9/13)
HinfI         166  GAATC                G^A[ACGT]TC
HphI          358  TCACC                GGTGA(8/7)
MaeIII        389  GTGAC                ^GT[ACGT]AC
MboII         378  GAAGA                GAAGA(8/7)
MslI           75  CACCACCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI           37  GCCTGCAGAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI           80  CCATGG               C^CATGG
PshAI         264  GACCACGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI           39  CTGCAG               CTGCA^G
RcaI          240  TCATGA               T^CATGA
RsaI          114  GTAC                 GT^AC
SfaNI           4  GCATC                GCATC(5/9)
SfcI           39  CTGCAG               C^T[GA][CT]AG
SmlI          171  CTTGAG               C^T[CT][GA]AG
SspBI         113  TGTACA               T^GTACA
StyI           80  CCATGG               C^C[AT][AT]GG
TfiI          166  GAATC                G^A[AT]TC
TseI           46  GCTGC                G^C[AT]GC
Tsp45I        389  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AccI           67  GTCTAC               GT^[AC][GT]AC
              223  GTAGAC              
AlwI           30  GGATC                GGATC(4/5)
              289  GGATC               
BsmFI         320  GGGAC                GGGAC(10/14)
              105  GTCCC               
Cfr13I        270  GGTCC                G^G[ACGT]CC
              413  GGGCC               
DsaI           80  CCATGG               C^C[GA][CT]GG
              266  CCACGG              
Sau96I        270  GGTCC                G^G[ACGT]CC
              413  GGGCC               
StuI           35  AGGCCT               AGG^CCT
              353  AGGCCT              
TspRI         293  CAGTG                CA[GC]TG[ACGT][ACGT]^
              371  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   19 enzymes) #
#########################################

AciI        BsaJI       BsiYI       BslI        BssKI       
BstNI       Cac8I       DpnII       EcoRII      Fnu4HI      
HaeIII      ItaI        MboI        MnlI        MvaI        
NdeII       NlaIII      Sau3AI      ScrFI