Restriction map for
pF1KB8752
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstPI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MaeII
MaeIII MamI MboI MfeI MflI
MluI MroI MseI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NdeI NdeII NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI TfiI Tru9I
Tsp45I Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 538 GTATAC GT^[AC][GT]AC
Alw44I 146 GTGCAC G^TGCAC
AlwNI 188 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 146 GTGCAC G^TGCAC
AspHI 146 GTGCAC G[AT]GC[AT]^C
BalI 94 TGGCCA TGG^CCA
BanII 460 GAGCCC G[GA]GC[CT]^C
BbsI 308 GAAGAC GAAGAC(2/6)
BciVI 286 GTATCC GTATCC(6/5)
BfaI 70 CTAG C^TAG
BpmI 323 CTCCAG CTGGAG(16/14)
BpuAI 308 GAAGAC GAAGAC(2/6)
BsaI 238 GAGACC GGTCTC(1/5)
BsgI 477 GTGCAG GTGCAG(16/14)
BsiHKAI 146 GTGCAC G[AT]GC[AT]^C
BsmFI 525 GTCCC GGGAC(10/14)
Bst1107I 538 GTATAC GTA^TAC
BstZ17I 538 GTATAC GTA^TAC
DraII 523 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 471 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 18 GACTTGTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 91 CCATGG C^C[GA][CT]GG
EaeI 94 TGGCCA [CT]^GGCC[GA]
EcoO109I 523 AGGTCCC [GA]G^G[ACGT]CC[CT]
HphI 319 TCACC GGTGA(8/7)
MaeI 70 CTAG C^TAG
MluNI 94 TGGCCA TGG^CCA
MscI 94 TGGCCA TGG^CCA
NcoI 91 CCATGG C^CATGG
PflMI 419 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 523 AGGTCCC [GA]G^G[AT]CC[CT]
SfcI 533 CTACAG C^T[GA][CT]AG
StuI 105 AGGCCT AGG^CCT
Tsp509I 383 AATT ^AATT
Van91I 419 CCACAGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AluI 206 AGCT AG^CT
327 AGCT
BmyI 146 GTGCAC G[AGT]GC[ACT]^C
460 GAGCCC
BsiYI 97 CCACCCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
419 CCACAGGATGG
BslI 97 CCACCCAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
419 CCACAGGATGG
Bsp1286I 146 GTGCAC G[AGT]GC[ACT]^C
460 GAGCCC
BstAPI 187 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
470 GCACAGGGTGC
DdeI 33 CTTAG C^T[ACGT]AG
506 CTGAG
EarI 75 CTCTTC CTCTTC(1/4)
315 CTCTTC
EcoT14I 91 CCATGG C^C[AT][AT]GG
108 CCTTGG
HaeIII 95 GGCC GG^CC
106 GGCC
HinfI 73 GACTC G^A[ACGT]TC
83 GACTC
Ksp632I 75 CTCTTC CTCTTC(1/4)
315 CTCTTC
MwoI 187 GCAGCCTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
470 GCACAGGGTGC
NlaIII 92 CATG CATG^
158 CATG
PleI 73 GACTC GAGTC(4/5)
83 GACTC
SfaNI 454 GATGC GCATC(5/9)
154 GCATC
StyI 91 CCATGG C^C[AT][AT]GG
108 CCTTGG
TspRI 142 CACTG CA[GC]TG[ACGT][ACGT]^
149 CACTG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AvaII BbvI BsaJI BsmAI BsrI
BssKI BstF5I BstNI Cfr13I EcoRII
Fnu4HI FokI ItaI MboII MnlI
MvaI NlaIV Sau96I ScrFI TseI