Restriction map for
pF1KB8753
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BlnI
BpmI BpuAI BsaBI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstF5I
BstPI BstXI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT22I
FbaI FokI FseI FspI HaeII
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MboII
MfeI MluI MroI MslI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tth111I TthHB8I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccII 568 CGCG CG^CG
AcsI 12 GAATTC [GA]^AATT[CT]
AcyI 340 GGCGTC G[GA]^CG[CT]C
AfaI 255 GTAC GT^AC
AflII 397 CTTAAG C^TTAAG
AflIII 167 ACGTGT A^C[GA][CT]GT
AlwNI 499 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 12 GAATTC [GA]^AATT[CT]
AvaII 435 GGACC G^G[AT]CC
BalI 359 TGGCCA TGG^CCA
BanI 375 GGCACC G^G[CT][GA]CC
BbrPI 166 CACGTG CAC^GTG
BcnI 453 CCGGG CC^[GC]GG
BfaI 421 CTAG C^TAG
BfrI 397 CTTAAG C^TTAAG
BglI 553 GCCCATGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 583 AGATCT A^GATCT
BsaHI 340 GGCGTC G[GA]^CG[CT]C
BsaI 115 GAGACC GGTCTC(1/5)
BsrBI 205 CCGCTC CCGCTC(-3/-3)
BsrI 472 ACTGG ACTGG(1/-1)
BssSI 250 CTCGTG CACGAG(-5/-1)
BstAPI 376 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 568 CGCG CG^CG
BstYI 583 AGATCT [GA]^GATC[CT]
CfoI 567 GCGC GCG^C
DsaI 555 CCATGG C^C[GA][CT]GG
EaeI 359 TGGCCA [CT]^GGCC[GA]
EcoRI 12 GAATTC G^AATTC
EcoRV 244 GATATC GAT^ATC
EcoT14I 555 CCATGG C^C[AT][AT]GG
HapII 453 CCGG C^CGG
HgaI 341 GCGTC GACGC(5/10)
HhaI 567 GCGC GCG^C
Hin1I 340 GGCGTC G[GA]^CG[CT]C
HinP1I 567 GCGC G^CGC
HpaII 453 CCGG C^CGG
HphI 45 GGTGA GGTGA(8/7)
MaeI 421 CTAG C^TAG
MflI 583 AGATCT [GA]^GATC[CT]
MluNI 359 TGGCCA TGG^CCA
MscI 359 TGGCCA TGG^CCA
MseI 398 TTAA T^TAA
MspI 453 CCGG C^CGG
MvnI 568 CGCG CG^CG
NciI 453 CCGGG CC^[GC]GG
NcoI 555 CCATGG C^CATGG
NspI 533 GCATGT [GA]CATG^[CT]
PmaCI 166 CACGTG CAC^GTG
PmlI 166 CACGTG CAC^GTG
RsaI 255 GTAC GT^AC
SfaNI 382 GATGC GCATC(5/9)
SmlI 397 CTTAAG C^T[CT][GA]AG
StyI 555 CCATGG C^C[AT][AT]GG
TfiI 428 GATTC G^A[AT]TC
Tru9I 398 TTAA T^TAA
Tsp509I 13 AATT ^AATT
XbaI 420 TCTAGA T^CTAGA
XhoII 583 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 304 GAGCTC G[AT]GC[AT]^C
448 GAGCTC
AvaI 289 CCCGAG C^[CT]CG[GA]G
487 CCCGAG
BlpI 301 GCTGAGC GC^T[ACGT]AGC
562 GCTGAGC
Bpu1102I 301 GCTGAGC GC^T[ACGT]AGC
562 GCTGAGC
BsaAI 166 CACGTG [CT]AC^GT[GA]
256 TACGTG
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
448 GAGCTC
BsmAI 115 GAGAC GTCTC(1/5)
572 GAGAC
BsmFI 185 GGGAC GGGAC(10/14)
343 GTCCC
BsoBI 289 CCCGAG C^[CT]CG[GA]G
487 CCCGAG
CelII 301 GCTGAGC GC^T[ACGT]AGC
562 GCTGAGC
DpnII 402 GATC ^GATC
584 GATC
DraII 200 AGGCCCC [GA]G^G[ACGT]CC[CT]
491 AGGCCCT
EcoO109I 200 AGGCCCC [GA]G^G[ACGT]CC[CT]
491 AGGCCCT
MaeIII 23 GTCAC ^GT[ACGT]AC
171 GTCAC
MboI 402 GATC ^GATC
584 GATC
NdeII 402 GATC ^GATC
584 GATC
PflMI 102 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
105 CCAGCTGGTGG
SacI 304 GAGCTC GAGCT^C
448 GAGCTC
Sau3AI 402 GATC ^GATC
584 GATC
Tsp45I 23 GTCAC ^GT[GC]AC
171 GTCAC
Van91I 102 CCACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
105 CCAGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I Cfr13I DdeI EcoRII
FauI Fnu4HI HaeIII HinfI ItaI
MaeII MnlI MspA1I MvaI MwoI
NlaIII NlaIV PleI PstI PvuII
Sau96I ScrFI SfcI TseI TspRI