Restriction map for
pF1KB8757
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# NO CUT ENZYMES ( 153 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AfaI AflII AflIII AgeI Alw44I
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspHI AspI AviII AvrII BamHI
BanII BbrPI BbsI BcgI BciVI
BclI BfrI BglI BglII BlnI
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FokI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeIII MamI MfeI MluI
MroI MseI MspA1I MunI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 226 GACGTC GACGT^C
AccII 200 CGCG CG^CG
AhdI 279 GACAGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 279 GACAGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 411 GGACC G^G[AT]CC
BalI 398 TGGCCA TGG^CCA
BbeI 24 GGCGCC GGCGC^C
BbvCI 436 GCTGAGG CCTCAGC(-5/-2)
BlpI 316 GCTGAGC GC^T[ACGT]AGC
BpmI 325 CTGGAG CTGGAG(16/14)
Bpu1102I 316 GCTGAGC GC^T[ACGT]AGC
BseRI 36 CTCCTC GAGGAG(10/8)
BsmAI 597 GAGAC GTCTC(1/5)
BsmBI 597 GAGACG CGTCTC(1/5)
BsrBI 319 GAGCGG CCGCTC(-3/-3)
BsrI 385 CCAGT ACTGG(1/-1)
BstNI 375 CCAGG CC^[AT]GG
BstUI 200 CGCG CG^CG
BstYI 176 AGATCC [GA]^GATC[CT]
CelII 316 GCTGAGC GC^T[ACGT]AGC
DraII 533 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 398 TGGCCA [CT]^GGCC[GA]
Eam1105I 279 GACAGACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 525 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 533 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 375 CCAGG ^CC[AT]GG
EcoT14I 335 CCAAGG C^C[AT][AT]GG
FauI 272 CCCGC CCCGC(4/6)
HgaI 304 GACGC GACGC(5/10)
HphI 209 TCACC GGTGA(8/7)
KasI 24 GGCGCC G^GCGCC
MaeII 227 ACGT A^CGT
MflI 176 AGATCC [GA]^GATC[CT]
MluNI 398 TGGCCA TGG^CCA
MscI 398 TGGCCA TGG^CCA
MslI 486 CACTCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 375 CCAGG CC^[AT]GG
MvnI 200 CGCG CG^CG
NarI 24 GGCGCC GG^CGCC
NheI 468 GCTAGC G^CTAGC
NspI 66 GCATGT [GA]CATG^[CT]
PleI 88 GACTC GAGTC(4/5)
SfaNI 206 GCATC GCATC(5/9)
SfoI 24 GGCGCC GGC^GCC
SmaI 418 CCCGGG CCC^GGG
StyI 335 CCAAGG C^C[AT][AT]GG
TfiI 353 GAATC G^A[AT]TC
XhoII 176 AGATCC [GA]^GATC[CT]
XmaCI 418 CCCGGG C^CCGGG
XmaI 418 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 24 GGCGCC G[GA]^CG[CT]C
226 GACGTC
Aor51HI 430 AGCGCT AGC^GCT
505 AGCGCT
BanI 24 GGCGCC G^G[CT][GA]CC
183 GGTGCC
BfaI 289 CTAG C^TAG
469 CTAG
BmyI 184 GTGCCC G[AGT]GC[ACT]^C
422 GGGCAC
BsaHI 24 GGCGCC G[GA]^CG[CT]C
226 GACGTC
BsmFI 224 GGGAC GGGAC(10/14)
148 GTCCC
Bsp1286I 184 GTGCCC G[AGT]GC[ACT]^C
422 GGGCAC
EarI 447 GAAGAG CTCTTC(1/4)
594 GAAGAG
Eco47III 430 AGCGCT AGC^GCT
505 AGCGCT
Hin1I 24 GGCGCC G[GA]^CG[CT]C
226 GACGTC
HinfI 88 GACTC G^A[ACGT]TC
353 GAATC
Ksp632I 447 GAAGAG CTCTTC(1/4)
594 GAAGAG
MaeI 289 CTAG C^TAG
469 CTAG
NlaIII 67 CATG CATG^
490 CATG
PstI 501 CTGCAG CTGCA^G
526 CTGCAG
SfcI 501 CTGCAG C^T[GA][CT]AG
526 CTGCAG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AlwI AvaI BbvI
BcnI BsaJI BsiYI BslI BsoBI
BssKI Cac8I CfoI Cfr13I DdeI
DpnII Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII ItaI MboI
MboII MnlI MspI MwoI NciI
NdeII NlaIV Sau3AI Sau96I ScrFI
SmlI TseI TspRI