Restriction map for
pF1KB8758
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# NO CUT ENZYMES ( 192 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AfaI AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssKI BssSI Bst1107I BstEII
BstNI BstPI BstUI BstYI BstZ17I
CelII CfoI Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRII
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI HpaII KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvaI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
PacI PaeR7I PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI Sau96I SbfI ScaI ScrFI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I TspRI
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 399 GTCTAC GT^[AC][GT]AC
AciI 502 GCGG CCGC(-3/-1)
AflII 291 CTTAAG C^TTAAG
AocI 213 CCTGAGG CC^T[ACGT]AGG
AspI 556 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BfaI 587 CTAG C^TAG
BfrI 291 CTTAAG C^TTAAG
BsgI 423 GTGCAG GTGCAG(16/14)
BsrI 299 ACTGG ACTGG(1/-1)
BstAPI 463 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 506 TTCGAA TT^CGAA
BstF5I 153 GGATG GGATG(2/0)
BstXI 473 CCACAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 213 CCTGAGG CC^T[ACGT]AGG
Cac8I 248 GCCTGC GC[ACGT]^[ACGT]GC
DpnII 538 GATC ^GATC
Eco81I 213 CCTGAGG CC^T[ACGT]AGG
EcoRI 310 GAATTC G^AATTC
EcoT14I 36 CCTTGG C^C[AT][AT]GG
FokI 153 GGATG GGATG(9/13)
HphI 570 TCACC GGTGA(8/7)
MaeI 587 CTAG C^TAG
MboI 538 GATC ^GATC
MslI 474 CACAAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 538 GATC ^GATC
NlaIII 171 CATG CATG^
NspV 506 TTCGAA TT^CGAA
PflFI 556 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 105 GCTCTTC GCTCTTC(1/4)
Sau3AI 538 GATC ^GATC
SfaNI 469 GATGC GCATC(5/9)
SfcI 401 CTACAG C^T[GA][CT]AG
SfuI 506 TTCGAA TT^CGAA
SmlI 291 CTTAAG C^T[CT][GA]AG
StyI 36 CCTTGG C^C[AT][AT]GG
Tth111I 556 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcsI 127 GAATTT [GA]^AATT[CT]
310 GAATTC
ApoI 127 GAATTT [GA]^AATT[CT]
310 GAATTC
Asp700I 306 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
499 GAAGCGGTTC
BsaJI 36 CCTTGG C^C[ACGT][ACGT]GG
524 CCGAGG
BsiYI 413 CCCAAGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
524 CCGAGGATAGG
BslI 413 CCCAAGCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
524 CCGAGGATAGG
BsmFI 182 GGGAC GGGAC(10/14)
428 GGGAC
BspMI 190 GCAGGT ACCTGC(4/8)
393 GCAGGT
DdeI 214 CTGAG C^T[ACGT]AG
341 CTGAG
DraI 22 TTTAAA TTT^AAA
132 TTTAAA
HindIII 279 AAGCTT A^AGCTT
416 AAGCTT
MwoI 463 GCAAAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
590 GCTCATCTTGC
TaqI 266 TCGA T^CGA
507 TCGA
TthHB8I 266 TCGA T^CGA
507 TCGA
XmnI 306 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
499 GAAGCGGTTC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BbvI EarI Fnu4HI HinfI
ItaI Ksp632I MaeIII MboII MnlI
MseI TfiI Tru9I TseI Tsp509I