Restriction map for
pF1KB8761
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# NO CUT ENZYMES ( 181 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BbvI BcgI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsoBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tru9I TseI
Tsp45I Tsp509I Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 299 CGCG CG^CG
BamHI 528 GGATCC G^GATCC
BanII 461 GGGCTC G[GA]GC[CT]^C
BbsI 138 GAAGAC GAAGAC(2/6)
BfaI 52 CTAG C^TAG
BglI 271 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 138 GAAGAC GAAGAC(2/6)
BsmI 145 GAATGC GAATGC(1/-1)
Bsp1407I 522 TGTACA T^GTACA
BsrGI 522 TGTACA T^GTACA
BsrI 195 ACTGG ACTGG(1/-1)
BstUI 299 CGCG CG^CG
BstYI 528 GGATCC [GA]^GATC[CT]
CfoI 315 GCGC GCG^C
DpnII 529 GATC ^GATC
DraIII 251 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 298 CCGCGG C^C[GA][CT]GG
FauI 300 GCGGG CCCGC(4/6)
HaeII 314 AGCGCC [GA]GCGC^[CT]
HhaI 315 GCGC GCG^C
HinP1I 315 GCGC G^CGC
KspI 298 CCGCGG CCGC^GG
MaeI 52 CTAG C^TAG
MboI 529 GATC ^GATC
MflI 528 GGATCC [GA]^GATC[CT]
MspA1I 298 CCGCGG C[AC]G^C[GT]G
MvnI 299 CGCG CG^CG
MwoI 271 GCCTCCCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 611 CATATG CA^TATG
NdeII 529 GATC ^GATC
NlaIII 129 CATG CATG^
PleI 388 GACTC GAGTC(4/5)
SacII 298 CCGCGG CCGC^GG
Sau3AI 529 GATC ^GATC
SspBI 522 TGTACA T^GTACA
TfiI 376 GATTC G^A[AT]TC
TspRI 423 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 528 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 86 GTAC GT^AC
523 GTAC
AluI 8 AGCT AG^CT
473 AGCT
AlwI 529 GATCC GGATC(4/5)
528 GGATC
BcnI 318 CCCGG CC^[GC]GG
338 CCCGG
BmyI 334 GTGCCC G[AGT]GC[ACT]^C
461 GGGCTC
BseRI 427 GAGGAG GAGGAG(10/8)
469 GAGGAG
BsiYI 294 CCAGCCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
359 CCGACAGGGGG
BslI 294 CCAGCCGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
359 CCGACAGGGGG
Bsp1286I 334 GTGCCC G[AGT]GC[ACT]^C
461 GGGCTC
EcoT14I 383 CCAAGG C^C[AT][AT]GG
541 CCAAGG
Fnu4HI 297 GCCGC GC^[ACGT]GC
304 GCCGC
HinfI 376 GATTC G^A[ACGT]TC
388 GACTC
HphI 181 GGTGA GGTGA(8/7)
238 GGTGA
ItaI 297 GCCGC GC^[ACGT]GC
304 GCCGC
NciI 318 CCCGG CC^[GC]GG
338 CCCGG
RsaI 86 GTAC GT^AC
523 GTAC
SfaNI 510 GATGC GCATC(5/9)
414 GCATC
StyI 383 CCAAGG C^C[AT][AT]GG
541 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI BsaJI BsmFI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI EcoRII
FokI HaeIII HapII HpaII MboII
MnlI MspI MvaI NlaIV Sau96I
ScrFI