Restriction map for
pF1KB8762
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AviII
AvrII BalI BamHI BbeI BbsI
BbvI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
EaeI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SspBI SspI StuI SwaI
Tru9I TseI Tsp509I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflIII 149 ACATGT A^C[GA][CT]GT
AhdI 67 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 67 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 283 GAGCTC G[AT]GC[AT]^C
BbrPI 550 CACGTG CAC^GTG
BbvCI 412 CCTCAGC CCTCAGC(-5/-2)
BclI 86 TGATCA T^GATCA
BsaAI 550 CACGTG [CT]AC^GT[GA]
BsaI 406 GAGACC GGTCTC(1/5)
BseRI 508 GAGGAG GAGGAG(10/8)
BsiHKAI 283 GAGCTC G[AT]GC[AT]^C
BspLU11I 149 ACATGT A^CATGT
BspMI 68 ACCTGC ACCTGC(4/8)
BstYI 620 AGATCC [GA]^GATC[CT]
DdeI 413 CTCAG C^T[ACGT]AG
DraIII 267 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 489 GACCCCGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 203 CCATGG C^C[GA][CT]GG
Eam1105I 67 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 86 TGATCA T^GATCA
HindIII 58 AAGCTT A^AGCTT
MaeII 551 ACGT A^CGT
MaeIII 146 GTGAC ^GT[ACGT]AC
MflI 620 AGATCC [GA]^GATC[CT]
MslI 6 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 97 CCGCTG C[AC]G^C[GT]G
NcoI 203 CCATGG C^CATGG
PflMI 460 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 496 GAGTC GAGTC(4/5)
PmaCI 550 CACGTG CAC^GTG
PmlI 550 CACGTG CAC^GTG
SacI 283 GAGCTC GAGCT^C
SbfI 171 CCTGCAGG CCTGCA^GG
SmlI 310 CTCAAG C^T[CT][GA]AG
SphI 614 GCATGC GCATG^C
Sse8387I 171 CCTGCAGG CCTGCA^GG
TaqI 236 TCGA T^CGA
TfiI 456 GATTC G^A[AT]TC
Tsp45I 146 GTGAC ^GT[GC]AC
TthHB8I 236 TCGA T^CGA
Van91I 460 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 620 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 21 enzymes) #
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AlwI 621 GATCC GGATC(4/5)
597 GGATC
AlwNI 461 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
601 CAGGACCTG
BanI 12 GGTGCC G^G[CT][GA]CC
229 GGCACC
BanII 283 GAGCTC G[GA]GC[CT]^C
511 GAGCCC
BpmI 403 CTGGAG CTGGAG(16/14)
450 CTGGAG
BsgI 397 CTGCAC GTGCAG(16/14)
547 CTGCAC
BsmAI 406 GAGAC GTCTC(1/5)
463 GAGAC
BsrI 466 ACTGG ACTGG(1/-1)
249 CCAGT
EcoT14I 8 CCAAGG C^C[AT][AT]GG
203 CCATGG
FauI 257 CCCGC CCCGC(4/6)
279 GCGGG
Fnu4HI 137 GCGGC GC^[ACGT]GC
140 GCGGC
HinfI 456 GATTC G^A[ACGT]TC
496 GAGTC
HphI 254 TCACC GGTGA(8/7)
356 TCACC
ItaI 137 GCGGC GC^[ACGT]GC
140 GCGGC
MboII 220 GAAGA GAAGA(8/7)
223 GAAGA
MwoI 131 GCAGTGGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
540 GCTGTCCCTGC
NspI 149 ACATGT [GA]CATG^[CT]
614 GCATGC
PpuMI 65 GGGACCT [GA]G^G[AT]CC[CT]
602 AGGACCT
PstI 172 CTGCAG CTGCA^G
586 CTGCAG
SfcI 172 CTGCAG C^T[GA][CT]AG
586 CTGCAG
StyI 8 CCAAGG C^C[AT][AT]GG
203 CCATGG
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AvaII BcnI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DpnII DraII EcoO109I EcoRII FokI
HaeIII HapII HpaII MboI MnlI
MspI MvaI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TspRI