Restriction map for
pF1KB8764
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HgaI Hin1I HindIII
HinfI HpaI KasI KpnI KspI
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI StuI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AfaI 634 GTAC GT^AC
ApaI 382 GGGCCC GGGCC^C
BalI 339 TGGCCA TGG^CCA
BamHI 366 GGATCC G^GATCC
BbsI 13 GAAGAC GAAGAC(2/6)
BcnI 155 CCCGG CC^[GC]GG
BfaI 187 CTAG C^TAG
BpuAI 13 GAAGAC GAAGAC(2/6)
BsgI 287 CTGCAC GTGCAG(16/14)
BsmAI 88 GAGAC GTCTC(1/5)
BsmFI 53 GGGAC GGGAC(10/14)
BssSI 197 CTCGTG CACGAG(-5/-1)
BstYI 366 GGATCC [GA]^GATC[CT]
CfoI 360 GCGC GCG^C
DpnII 367 GATC ^GATC
EaeI 339 TGGCCA [CT]^GGCC[GA]
HaeII 359 AGCGCC [GA]GCGC^[CT]
HapII 156 CCGG C^CGG
HhaI 360 GCGC GCG^C
HinP1I 360 GCGC G^CGC
HincII 271 GTCAAC GT[CT]^[GA]AC
HindII 271 GTCAAC GT[CT]^[GA]AC
HpaII 156 CCGG C^CGG
MaeI 187 CTAG C^TAG
MaeII 275 ACGT A^CGT
MaeIII 410 GTAAC ^GT[ACGT]AC
MboI 367 GATC ^GATC
MflI 366 GGATCC [GA]^GATC[CT]
MluNI 339 TGGCCA TGG^CCA
MscI 339 TGGCCA TGG^CCA
MslI 129 CACTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 607 CAGCGG C[AC]G^C[GT]G
MspI 156 CCGG C^CGG
NciI 155 CCCGG CC^[GC]GG
NdeII 367 GATC ^GATC
PstI 626 CTGCAG CTGCA^G
RsaI 634 GTAC GT^AC
Sau3AI 367 GATC ^GATC
SfaNI 300 GCATC GCATC(5/9)
SfcI 626 CTGCAG C^T[GA][CT]AG
SfiI 374 GGCCCCACGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspI 176 AATATT AAT^ATT
XcmI 342 CCATGGTCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 366 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AlwI 367 GATCC GGATC(4/5)
366 GGATC
BanII 382 GGGCCC G[GA]GC[CT]^C
536 GAGCCC
BglI 236 GCCTGTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
375 GCCCCACGGGC
DraII 373 AGGCCCC [GA]G^G[ACGT]CC[CT]
382 GGGCCCC
EcoNI 426 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
540 CCTGGAGGAGG
EcoO109I 373 AGGCCCC [GA]G^G[ACGT]CC[CT]
382 GGGCCCC
HphI 252 GGTGA GGTGA(8/7)
582 GGTGA
NcoI 342 CCATGG C^CATGG
443 CCATGG
NspI 311 GCATGC [GA]CATG^[CT]
601 GCATGC
SphI 311 GCATGC GCATG^C
601 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI BanI BbvI BmyI
BpmI BsaJI BseRI BsiYI BslI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DsaI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII ItaI Ksp632I MboII
MnlI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI StyI TseI TspRI