Restriction map for
pF1KB8767
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BbeI BbrPI
BbvCI BciVI BclI BfaI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI Hin1I HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MluI MroI MseI
MslI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 211 CGCG CG^CG
Alw44I 370 GTGCAC G^TGCAC
ApaI 634 GGGCCC GGGCC^C
ApaLI 370 GTGCAC G^TGCAC
AspI 255 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 442 CCCGAG C^[CT]CG[GA]G
BalI 557 TGGCCA TGG^CCA
BbsI 582 GAAGAC GAAGAC(2/6)
BlpI 243 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 243 GCTCAGC GC^T[ACGT]AGC
BpuAI 582 GAAGAC GAAGAC(2/6)
BsoBI 442 CCCGAG C^[CT]CG[GA]G
BsrBI 129 GAGCGG CCGCTC(-3/-3)
BstAPI 333 GCAGCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3 GGTCACC G^GT[ACGT]ACC
BstPI 3 GGTCACC G^GT[ACGT]ACC
BstUI 211 CGCG CG^CG
BstYI 518 AGATCC [GA]^GATC[CT]
CelII 243 GCTCAGC GC^T[ACGT]AGC
DraII 633 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 633 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 3 GGTCACC G^GT[ACGT]ACC
HaeII 227 AGCGCC [GA]GCGC^[CT]
HgaI 248 GCGTC GACGC(5/10)
HincII 589 GTCAAC GT[CT]^[GA]AC
HindII 589 GTCAAC GT[CT]^[GA]AC
MaeII 554 ACGT A^CGT
MflI 518 AGATCC [GA]^GATC[CT]
MluNI 557 TGGCCA TGG^CCA
MscI 557 TGGCCA TGG^CCA
MvnI 211 CGCG CG^CG
NlaIII 381 CATG CATG^
NspI 380 ACATGC [GA]CATG^[CT]
PflFI 255 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 485 GAAGAGC GCTCTTC(1/4)
TaqI 350 TCGA T^CGA
Tth111I 255 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 350 TCGA T^CGA
XcmI 459 CCAGCCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 518 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 90 GTAC GT^AC
660 GTAC
AviII 113 TGCGCA TGC^GCA
278 TGCGCA
BanI 196 GGCACC G^G[CT][GA]CC
438 GGTGCC
BcgI 167 CGAACCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
570 GCATCTCTGTCG
BpmI 141 CTGGAG CTGGAG(16/14)
392 CTGGAG
BseRI 429 CTCCTC GAGGAG(10/8)
546 CTCCTC
BsmFI 265 GGGAC GGGAC(10/14)
476 GTCCC
BspMI 170 ACCTGC ACCTGC(4/8)
275 ACCTGC
BstF5I 282 CATCC GGATG(2/0)
32 GGATG
DdeI 178 CTCAG C^T[ACGT]AG
244 CTCAG
EaeI 557 TGGCCA [CT]^GGCC[GA]
594 CGGCCA
EarI 563 CTCTTC CTCTTC(1/4)
485 GAAGAG
FokI 282 CATCC GGATG(9/13)
32 GGATG
FspI 113 TGCGCA TGC^GCA
278 TGCGCA
HinfI 474 GAGTC G^A[ACGT]TC
604 GACTC
Ksp632I 563 CTCTTC CTCTTC(1/4)
485 GAAGAG
MspA1I 94 CAGCTG C[AC]G^C[GT]G
625 CAGCTG
NaeI 193 GCCGGC GCC^GGC
536 GCCGGC
NgoMIV 193 GCCGGC G^CCGGC
536 GCCGGC
PflMI 93 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
463 CCACTCCCTGG
PleI 604 GACTC GAGTC(4/5)
474 GAGTC
PstI 121 CTGCAG CTGCA^G
310 CTGCAG
PvuII 94 CAGCTG CAG^CTG
625 CAGCTG
RsaI 90 GTAC GT^AC
660 GTAC
SfcI 121 CTGCAG C^T[GA][CT]AG
310 CTGCAG
SmlI 64 CTCAAG C^T[CT][GA]AG
361 CTCAAG
Van91I 93 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
463 CCACTCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanII BbvI BcnI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrFI
BsrI BssKI BstNI Cac8I CfoI
Cfr10I Cfr13I DpnII EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIV Sau3AI Sau96I
ScrFI SfaNI TseI Tsp45I TspRI