Restriction map for
pF1KB8769
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# NO CUT ENZYMES ( 164 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeI MaeIII
MamI MfeI MluI MroI MseI
MslI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TfiI
Tru9I Tsp45I TspRI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccII 154 CGCG CG^CG
AcsI 167 AAATTT [GA]^AATT[CT]
AfaI 372 GTAC GT^AC
AluI 131 AGCT AG^CT
AlwNI 244 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 668 AGCGCT AGC^GCT
ApaI 643 GGGCCC GGGCC^C
ApoI 167 AAATTT [GA]^AATT[CT]
AvaI 686 CCCGAG C^[CT]CG[GA]G
AvaII 80 GGTCC G^G[AT]CC
BcnI 237 CCGGG CC^[GC]GG
BglI 12 GCCATTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsmI 564 GAATGC GAATGC(1/-1)
BsoBI 686 CCCGAG C^[CT]CG[GA]G
BsrDI 615 CATTGC GCAATG(2/0)
BsrI 101 CCAGT ACTGG(1/-1)
BstUI 154 CGCG CG^CG
CfoI 669 GCGC GCG^C
Eco47III 668 AGCGCT AGC^GCT
FauI 9 CCCGC CCCGC(4/6)
HaeII 668 AGCGCT [GA]GCGC^[CT]
HhaI 669 GCGC GCG^C
HinP1I 669 GCGC G^CGC
HphI 48 GGTGA GGTGA(8/7)
MaeII 544 ACGT A^CGT
MvnI 154 CGCG CG^CG
NciI 237 CCGGG CC^[GC]GG
NlaIII 229 CATG CATG^
NruI 153 TCGCGA TCG^CGA
PflMI 243 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 133 CTGCAG CTGCA^G
RsaI 372 GTAC GT^AC
SfcI 133 CTGCAG C^T[GA][CT]AG
SmlI 511 CTTGAG C^T[CT][GA]AG
StuI 398 AGGCCT AGG^CCT
TaqI 635 TCGA T^CGA
Tsp509I 168 AATT ^AATT
TthHB8I 635 TCGA T^CGA
Van91I 243 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 225 GATCC GGATC(4/5)
246 GATCC
Asp700I 291 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
354 GAAAACTTTC
BalI 2 TGGCCA TGG^CCA
609 TGGCCA
BanII 503 GAGCCC G[GA]GC[CT]^C
643 GGGCCC
BbvI 666 GCAGC GCAGC(8/12)
132 GCTGC
BmyI 503 GAGCCC G[AGT]GC[ACT]^C
643 GGGCCC
BsaI 270 GAGACC GGTCTC(1/5)
434 GAGACC
BsaWI 77 TCCGGT [AT]^CCGG[AT]
437 ACCGGA
BseRI 265 GAGGAG GAGGAG(10/8)
286 GAGGAG
BsmAI 270 GAGAC GTCTC(1/5)
434 GAGAC
BsmFI 81 GTCCC GGGAC(10/14)
546 GTCCC
Bsp1286I 503 GAGCCC G[AGT]GC[ACT]^C
643 GGGCCC
BstAPI 126 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
666 GCAGCGCTTGC
BstNI 84 CCTGG CC^[AT]GG
249 CCTGG
BstYI 224 AGATCC [GA]^GATC[CT]
245 AGATCC
DpnII 225 GATC ^GATC
246 GATC
EaeI 2 TGGCCA [CT]^GGCC[GA]
609 TGGCCA
EcoRII 84 CCTGG ^CC[AT]GG
249 CCTGG
MboI 225 GATC ^GATC
246 GATC
MflI 224 AGATCC [GA]^GATC[CT]
245 AGATCC
MluNI 2 TGGCCA TGG^CCA
609 TGGCCA
MscI 2 TGGCCA TGG^CCA
609 TGGCCA
MvaI 84 CCTGG CC^[AT]GG
249 CCTGG
NdeII 225 GATC ^GATC
246 GATC
Sau3AI 225 GATC ^GATC
246 GATC
SfaNI 630 GATGC GCATC(5/9)
91 GCATC
TseI 132 GCTGC G^C[AT]GC
666 GCAGC
XhoII 224 AGATCC [GA]^GATC[CT]
245 AGATCC
XmnI 291 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
354 GAAAACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI BsaJI BsiYI BslI BssKI
BstF5I Cac8I Cfr13I DraII EarI
EcoNI EcoO109I Fnu4HI FokI HaeIII
HapII HpaII ItaI Ksp632I MboII
MnlI MspI MwoI NlaIV Sau96I
ScrFI