Restriction map for
pF1KB8770
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MluI MroI
MslI MvnI NarI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AcsI 271 GAATTT [GA]^AATT[CT]
AflII 442 CTTAAG C^TTAAG
AflIII 692 ACACGT A^C[GA][CT]GT
Alw44I 252 GTGCAC G^TGCAC
ApaLI 252 GTGCAC G^TGCAC
ApoI 271 GAATTT [GA]^AATT[CT]
BalI 569 TGGCCA TGG^CCA
BanII 654 GAGCTC G[GA]GC[CT]^C
BbsI 621 GAAGAC GAAGAC(2/6)
BbvCI 675 CCTCAGC CCTCAGC(-5/-2)
BfrI 442 CTTAAG C^TTAAG
BglII 5 AGATCT A^GATCT
BpmI 631 CTGGAG CTGGAG(16/14)
BpuAI 621 GAAGAC GAAGAC(2/6)
BseRI 226 GAGGAG GAGGAG(10/8)
BsmFI 504 GTCCC GGGAC(10/14)
BsrFI 289 GCCGGC [GA]^CCGG[CT]
BstF5I 321 GGATG GGATG(2/0)
BstYI 5 AGATCT [GA]^GATC[CT]
CfoI 360 GCGC GCG^C
Cfr10I 289 GCCGGC [GA]^CCGG[CT]
DpnII 6 GATC ^GATC
DraII 596 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 566 CCATGG C^C[GA][CT]GG
EaeI 569 TGGCCA [CT]^GGCC[GA]
EcoNI 57 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 596 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 566 CCATGG C^C[AT][AT]GG
FokI 321 GGATG GGATG(9/13)
HhaI 360 GCGC GCG^C
HinP1I 360 GCGC G^CGC
MaeII 694 ACGT A^CGT
MboI 6 GATC ^GATC
MfeI 259 CAATTG C^AATTG
MflI 5 AGATCT [GA]^GATC[CT]
MluNI 569 TGGCCA TGG^CCA
MscI 569 TGGCCA TGG^CCA
MspA1I 395 CAGCTG C[AC]G^C[GT]G
MunI 259 CAATTG C^AATTG
NaeI 289 GCCGGC GCC^GGC
NcoI 566 CCATGG C^CATGG
NdeII 6 GATC ^GATC
NgoMIV 289 GCCGGC G^CCGGC
NlaIV 597 GGACCC GG[ACGT]^[ACGT]CC
PflMI 561 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 329 GAGTC GAGTC(4/5)
PpuMI 596 AGGACCC [GA]G^G[AT]CC[CT]
PstI 201 CTGCAG CTGCA^G
PvuII 395 CAGCTG CAG^CTG
SacI 654 GAGCTC GAGCT^C
Sau3AI 6 GATC ^GATC
SfaNI 233 GCATC GCATC(5/9)
SmaI 160 CCCGGG CCC^GGG
StyI 566 CCATGG C^C[AT][AT]GG
TspRI 554 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 561 CCAGACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 5 AGATCT [GA]^GATC[CT]
XmaCI 160 CCCGGG C^CCGGG
XmaI 160 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AvaI 160 CCCGGG C^[CT]CG[GA]G
583 CCCGAG
AvaII 264 GGACC G^G[AT]CC
597 GGACC
BbvI 43 GCTGC GCAGC(8/12)
211 GCTGC
BsaI 532 GAGACC GGTCTC(1/5)
105 GGTCTC
BsoBI 160 CCCGGG C^[CT]CG[GA]G
583 CCCGAG
BsrI 39 ACTGG ACTGG(1/-1)
437 CCAGT
Cac8I 155 GCAGGC GC[ACGT]^[ACGT]GC
289 GCCGGC
Fnu4HI 43 GCTGC GC^[ACGT]GC
211 GCTGC
HaeIII 158 GGCC GG^CC
570 GGCC
HphI 17 TCACC GGTGA(8/7)
182 TCACC
ItaI 43 GCTGC GC^[ACGT]GC
211 GCTGC
MaeIII 181 GTCAC ^GT[ACGT]AC
556 GTGAC
MseI 143 TTAA T^TAA
443 TTAA
MwoI 571 GCCACCAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
656 GCTCTCTCTGC
TfiI 354 GAATC G^A[AT]TC
433 GAATC
Tru9I 143 TTAA T^TAA
443 TTAA
TseI 43 GCTGC G^C[AT]GC
211 GCTGC
Tsp45I 181 GTCAC ^GT[GC]AC
556 GTGAC
Tsp509I 260 AATT ^AATT
272 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI AspHI BcnI BfaI BmyI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BssKI BstNI Cfr13I
DdeI EcoRII HapII HinfI HpaII
MaeI MboII MnlI MspI MvaI
NciI NlaIII Sau96I ScrFI SfcI
SmlI