Restriction map for
pF1KB8773
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BamHI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
DraI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HinfI HpaI KpnI KspI
MamI MfeI MluI MroI MseI
MunI MvnI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RcaI SacI
SacII SalI SbfI SexAI SfaNI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
XbaI XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 267 GGCGCC G[GA]^CG[CT]C
AfaI 580 GTAC GT^AC
AflIII 116 ACATGT A^C[GA][CT]GT
ApaI 612 GGGCCC GGGCC^C
AspI 75 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 33 TGGCCA TGG^CCA
BbeI 267 GGCGCC GGCGC^C
BbrPI 697 CACGTG CAC^GTG
BbvCI 398 CCTCAGC CCTCAGC(-5/-2)
BclI 486 TGATCA T^GATCA
BcnI 610 CCGGG CC^[GC]GG
BfaI 659 CTAG C^TAG
BsaAI 697 CACGTG [CT]AC^GT[GA]
BsaHI 267 GGCGCC G[GA]^CG[CT]C
BseRI 211 GAGGAG GAGGAG(10/8)
BsgI 308 GTGCAG GTGCAG(16/14)
BsiEI 276 CGACCG CG[GA][CT]^CG
BspLU11I 116 ACATGT A^CATGT
BspMI 419 ACCTGC ACCTGC(4/8)
BsrDI 183 CATTGC GCAATG(2/0)
BsrFI 64 GCCGGC [GA]^CCGG[CT]
BstAPI 331 GCACATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 521 CCAGCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 64 GCCGGC [GA]^CCGG[CT]
CpoI 194 CGGTCCG CG^G[AT]CCG
DraIII 697 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 277 GACCGCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 290 CCACGG C^C[GA][CT]GG
EcoT14I 412 CCAAGG C^C[AT][AT]GG
FauI 264 GCGGG CCCGC(4/6)
FbaI 486 TGATCA T^GATCA
HgaI 219 GACGC GACGC(5/10)
Hin1I 267 GGCGCC G[GA]^CG[CT]C
HindIII 468 AAGCTT A^AGCTT
KasI 267 GGCGCC G^GCGCC
MaeI 659 CTAG C^TAG
MluNI 33 TGGCCA TGG^CCA
MscI 33 TGGCCA TGG^CCA
MslI 147 CATGCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 64 GCCGGC GCC^GGC
NarI 267 GGCGCC GG^CGCC
NciI 610 CCGGG CC^[GC]GG
NgoMIV 64 GCCGGC G^CCGGC
PflFI 75 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 696 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 697 CACGTG CAC^GTG
PmlI 697 CACGTG CAC^GTG
RsaI 580 GTAC GT^AC
RsrII 194 CGGTCCG CG^G[AT]CCG
SapI 532 GAAGAGC GCTCTTC(1/4)
ScaI 579 AGTACT AGT^ACT
SfoI 267 GGCGCC GGC^GCC
StyI 412 CCAAGG C^C[AT][AT]GG
Tth111I 75 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 696 CCACGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 374 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
429 CAGCGCCTG
AvaI 452 CTCGAG C^[CT]CG[GA]G
628 CTCGAG
BbsI 43 GAAGAC GAAGAC(2/6)
72 GAAGAC
BpmI 663 CTCCAG CTGGAG(16/14)
505 CTGGAG
BpuAI 43 GAAGAC GAAGAC(2/6)
72 GAAGAC
BsaI 718 GAGACC GGTCTC(1/5)
285 GGTCTC
BsmFI 229 GGGAC GGGAC(10/14)
353 GGGAC
BsoBI 452 CTCGAG C^[CT]CG[GA]G
628 CTCGAG
BsrI 373 CCAGT ACTGG(1/-1)
391 CCAGT
BssSI 8 CACGAG CACGAG(-5/-1)
37 CACGAG
DraII 612 GGGCCCC [GA]G^G[ACGT]CC[CT]
654 AGGCCCT
EarI 18 GAAGAG CTCTTC(1/4)
532 GAAGAG
EcoO109I 612 GGGCCCC [GA]G^G[ACGT]CC[CT]
654 AGGCCCT
HaeII 267 GGCGCC [GA]GCGC^[CT]
430 AGCGCC
HphI 126 GGTGA GGTGA(8/7)
395 TCACC
Ksp632I 18 GAAGAG CTCTTC(1/4)
532 GAAGAG
MaeIII 394 GTCAC ^GT[ACGT]AC
480 GTCAC
NspI 116 ACATGT [GA]CATG^[CT]
146 ACATGC
PaeR7I 452 CTCGAG C^TCGAG
628 CTCGAG
PvuII 522 CAGCTG CAG^CTG
666 CAGCTG
SmlI 452 CTCGAG C^T[CT][GA]AG
628 CTCGAG
Tsp45I 394 GTCAC ^GT[GC]AC
480 GTCAC
TspRI 77 CAGTG CA[GC]TG[ACGT][ACGT]^
242 CAGTG
XhoI 452 CTCGAG C^TCGAG
628 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BanII BbvI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrBI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII ItaI MaeII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfcI
TaqI TseI TthHB8I XhoII