Restriction map for
pF1KB8775
##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwI AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI BsmI BsoBI
Bsp1407I BspEI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII CfoI Cfr10I
CpoI DraI DraII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MroI MseI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AciI 28 GCGG CCGC(-3/-1)
AflIII 368 ACACGT A^C[GA][CT]GT
AocI 593 CCTTAGG CC^T[ACGT]AGG
AspI 475 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 654 GGTCC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BanI 729 GGCACC G^G[CT][GA]CC
BbsI 19 GAAGAC GAAGAC(2/6)
BcnI 546 CCGGG CC^[GC]GG
BpuAI 19 GAAGAC GAAGAC(2/6)
BsaJI 630 CCTTGG C^C[ACGT][ACGT]GG
BsiYI 332 CCCCCAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 332 CCCCCAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 208 GAGACG CGTCTC(1/5)
BspDI 74 ATCGAT AT^CGAT
BspHI 708 TCATGA T^CATGA
BspMI 200 GCAGGT ACCTGC(4/8)
Bsu36I 593 CCTTAGG CC^T[ACGT]AGG
Cfr13I 654 GGTCC G^G[ACGT]CC
ClaI 74 ATCGAT AT^CGAT
DpnII 150 GATC ^GATC
DraIII 173 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCA [CT]^GGCC[GA]
Eco81I 593 CCTTAGG CC^T[ACGT]AGG
EcoT14I 630 CCTTGG C^C[AT][AT]GG
HapII 546 CCGG C^CGG
HincII 8 GTCAAC GT[CT]^[GA]AC
HindII 8 GTCAAC GT[CT]^[GA]AC
HpaII 546 CCGG C^CGG
MboI 150 GATC ^GATC
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MspI 546 CCGG C^CGG
MwoI 564 GCTAGGTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 546 CCGGG CC^[GC]GG
NdeII 150 GATC ^GATC
PflFI 475 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 198 CTGCAG CTGCA^G
RcaI 708 TCATGA T^CATGA
Sau3AI 150 GATC ^GATC
Sau96I 654 GGTCC G^G[ACGT]CC
SfcI 198 CTGCAG C^T[GA][CT]AG
StyI 630 CCTTGG C^C[AT][AT]GG
TfiI 599 GAATC G^A[AT]TC
Tth111I 475 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 717 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 451 GAATTT [GA]^AATT[CT]
712 GAATTT
ApoI 451 GAATTT [GA]^AATT[CT]
712 GAATTT
AspHI 417 GAGCTC G[AT]GC[AT]^C
432 GAGCTC
BanII 417 GAGCTC G[GA]GC[CT]^C
432 GAGCTC
BbvI 321 GCTGC GCAGC(8/12)
324 GCTGC
BfaI 565 CTAG C^TAG
718 CTAG
BmyI 417 GAGCTC G[AGT]GC[ACT]^C
432 GAGCTC
BpmI 52 CTGGAG CTGGAG(16/14)
109 CTGGAG
BsgI 516 CTGCAC GTGCAG(16/14)
406 GTGCAG
BsiHKAI 417 GAGCTC G[AT]GC[AT]^C
432 GAGCTC
BsmAI 208 GAGAC GTCTC(1/5)
33 GTCTC
Bsp1286I 417 GAGCTC G[AGT]GC[ACT]^C
432 GAGCTC
BstNI 384 CCTGG CC^[AT]GG
651 CCTGG
EcoRII 384 CCTGG ^CC[AT]GG
651 CCTGG
Fnu4HI 321 GCTGC GC^[ACGT]GC
324 GCTGC
ItaI 321 GCTGC GC^[ACGT]GC
324 GCTGC
MaeI 565 CTAG C^TAG
718 CTAG
MboII 19 GAAGA GAAGA(8/7)
291 GAAGA
MvaI 384 CCTGG CC^[AT]GG
651 CCTGG
NlaIII 399 CATG CATG^
709 CATG
NlaIV 654 GGTCCC GG[ACGT]^[ACGT]CC
729 GGCACC
SacI 417 GAGCTC GAGCT^C
432 GAGCTC
StuI 38 AGGCCT AGG^CCT
460 AGGCCT
TseI 321 GCTGC G^C[AT]GC
324 GCTGC
Tsp509I 452 AATT ^AATT
713 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AluI BsmFI BsrI BssKI BstF5I
BstXI Cac8I DdeI FokI HaeIII
HinfI HphI MaeII MaeIII MnlI
MslI PleI ScrFI TaqI Tsp45I
TspRI TthHB8I