Restriction map for
pF1KB8777
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MamI MfeI
MluI MroI MseI MspA1I MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccII 324 CGCG CG^CG
ApaI 766 GGGCCC GGGCC^C
AspHI 199 GTGCTC G[AT]GC[AT]^C
BalI 614 TGGCCA TGG^CCA
BamHI 380 GGATCC G^GATCC
BanII 766 GGGCCC G[GA]GC[CT]^C
BbeI 88 GGCGCC GGCGC^C
BbsI 142 GTCTTC GAAGAC(2/6)
BglI 295 GCCATCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 224 AGATCT A^GATCT
BpmI 709 CTGGAG CTGGAG(16/14)
BpuAI 142 GTCTTC GAAGAC(2/6)
BsaI 39 GGTCTC GGTCTC(1/5)
BsiHKAI 199 GTGCTC G[AT]GC[AT]^C
BspMI 257 ACCTGC ACCTGC(4/8)
BstEII 624 GGTCACC G^GT[ACGT]ACC
BstPI 624 GGTCACC G^GT[ACGT]ACC
BstUI 324 CGCG CG^CG
BstXI 605 CCACAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 647 CCATGG C^C[GA][CT]GG
EcoO65I 624 GGTCACC G^GT[ACGT]ACC
KasI 88 GGCGCC G^GCGCC
MaeIII 625 GTCAC ^GT[ACGT]AC
MluNI 614 TGGCCA TGG^CCA
MscI 614 TGGCCA TGG^CCA
MvnI 324 CGCG CG^CG
NarI 88 GGCGCC GG^CGCC
NcoI 647 CCATGG C^CATGG
SbfI 456 CCTGCAGG CCTGCA^GG
SfoI 88 GGCGCC GGC^GCC
Sse8387I 456 CCTGCAGG CCTGCA^GG
Tsp45I 625 GTCAC ^GT[GC]AC
XcmI 386 CCAAGGACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcyI 88 GGCGCC G[GA]^CG[CT]C
391 GACGCC
AfaI 669 GTAC GT^AC
714 GTAC
BsaHI 88 GGCGCC G[GA]^CG[CT]C
391 GACGCC
BseRI 67 CTCCTC GAGGAG(10/8)
532 GAGGAG
BsrFI 110 GCCGGT [GA]^CCGG[CT]
263 ACCGGC
Cfr10I 110 GCCGGT [GA]^CCGG[CT]
263 ACCGGC
EarI 61 CTCTTC CTCTTC(1/4)
70 CTCTTC
EcoNI 162 CCTTCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
456 CCTGCAGGAGG
HgaI 391 GACGC GACGC(5/10)
590 GCGTC
Hin1I 88 GGCGCC G[GA]^CG[CT]C
391 GACGCC
HinfI 52 GAGTC G^A[ACGT]TC
493 GACTC
HphI 651 GGTGA GGTGA(8/7)
626 TCACC
Ksp632I 61 CTCTTC CTCTTC(1/4)
70 CTCTTC
MaeII 611 ACGT A^CGT
662 ACGT
MslI 519 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
606 CACAGACGTG
PleI 493 GACTC GAGTC(4/5)
52 GAGTC
PpuMI 170 AGGTCCC [GA]G^G[AT]CC[CT]
749 AGGACCC
RsaI 669 GTAC GT^AC
714 GTAC
SmaI 47 CCCGGG CCC^GGG
762 CCCGGG
TaqI 212 TCGA T^CGA
299 TCGA
TspRI 442 CACTG CA[GC]TG[ACGT][ACGT]^
524 CAGTG
TthHB8I 212 TCGA T^CGA
299 TCGA
XmaCI 47 CCCGGG C^CCGGG
762 CCCGGG
XmaI 47 CCCGGG C^CCGGG
762 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaI
AvaII BanI BbvI BcnI BmyI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HpaII ItaI MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TseI XhoII