Restriction map for
pF1KB8778
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AgeI AhdI Alw44I
AlwI AocI ApaLI ApoI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DpnII DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HpaI HphI KpnI Ksp632I
MamI MboI MfeI MflI MluNI
MscI MseI MslI MunI NaeI
NdeI NdeII NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsaI RsrII SalI SapI
Sau3AI SbfI ScaI SexAI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 285 TCCGGA T^CCGGA
AcyI 424 GGCGCC G[GA]^CG[CT]C
AflIII 170 ACGCGT A^C[GA][CT]GT
Aor51HI 290 AGCGCT AGC^GCT
AscI 255 GGCGCGCC GG^CGCGCC
AspHI 703 GAGCTC G[AT]GC[AT]^C
BanI 424 GGCGCC G^G[CT][GA]CC
BbeI 424 GGCGCC GGCGC^C
BbsI 606 GAAGAC GAAGAC(2/6)
BbvCI 153 CCTCAGC CCTCAGC(-5/-2)
BglI 199 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 388 CTCCAG CTGGAG(16/14)
BpuAI 606 GAAGAC GAAGAC(2/6)
BsaHI 424 GGCGCC G[GA]^CG[CT]C
BsaI 712 GAGACC GGTCTC(1/5)
BsaWI 285 TCCGGA [AT]^CCGG[AT]
BseAI 285 TCCGGA T^CCGGA
BsgI 174 GTGCAG GTGCAG(16/14)
BsiHKAI 703 GAGCTC G[AT]GC[AT]^C
BsmAI 712 GAGAC GTCTC(1/5)
BspEI 285 TCCGGA T^CCGGA
BspMI 333 GCAGGT ACCTGC(4/8)
BsrFI 114 GCCGGT [GA]^CCGG[CT]
BssHII 256 GCGCGC G^CGCGC
BstAPI 183 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 283 CATCC GGATG(2/0)
BstNI 390 CCAGG CC^[AT]GG
Cfr10I 114 GCCGGT [GA]^CCGG[CT]
Eco47III 290 AGCGCT AGC^GCT
EcoNI 596 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 390 CCAGG ^CC[AT]GG
FokI 283 CATCC GGATG(9/13)
Hin1I 424 GGCGCC G[GA]^CG[CT]C
KasI 424 GGCGCC G^GCGCC
KspI 276 CCGCGG CCGC^GG
MaeII 104 ACGT A^CGT
MaeIII 371 GTCAC ^GT[ACGT]AC
MboII 606 GAAGA GAAGA(8/7)
MluI 170 ACGCGT A^CGCGT
MroI 285 TCCGGA T^CCGGA
MvaI 390 CCAGG CC^[AT]GG
NarI 424 GGCGCC GG^CGCC
NcoI 659 CCATGG C^CATGG
NheI 97 GCTAGC G^CTAGC
NlaIII 660 CATG CATG^
PflMI 547 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 556 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 177 CAGCTG CAG^CTG
SacI 703 GAGCTC GAGCT^C
SacII 276 CCGCGG CCGC^GG
SfaNI 282 GCATC GCATC(5/9)
SfoI 424 GGCGCC GGC^GCC
TfiI 482 GATTC G^A[AT]TC
Tsp45I 371 GTCAC ^GT[GC]AC
Tsp509I 760 AATT ^AATT
Van91I 547 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AviII 295 TGCGCA TGC^GCA
350 TGCGCA
BfaI 98 CTAG C^TAG
439 CTAG
BsrBI 308 CCGCTC CCGCTC(-3/-3)
385 CCGCTC
BsrI 130 ACTGG ACTGG(1/-1)
264 CCAGT
DraII 364 AGGCCCT [GA]G^G[ACGT]CC[CT]
556 GGGACCC
EcoO109I 364 AGGCCCT [GA]G^G[ACGT]CC[CT]
556 GGGACCC
EcoT14I 635 CCTTGG C^C[AT][AT]GG
659 CCATGG
FspI 295 TGCGCA TGC^GCA
350 TGCGCA
MaeI 98 CTAG C^TAG
439 CTAG
PleI 3 GACTC GAGTC(4/5)
493 GAGTC
StyI 635 CCTTGG C^C[AT][AT]GG
659 CCATGG
TspRI 129 CACTG CA[GC]TG[ACGT][ACGT]^
265 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AluI AlwNI ApaI
AvaI AvaII BanII BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BssKI BstUI
Cac8I CfoI Cfr13I DdeI DsaI
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI MnlI MspA1I MspI MvnI
MwoI NciI NlaIV Sau96I ScrFI
SmaI TseI XmaCI XmaI