Restriction map for
pF1KB8779
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# NO CUT ENZYMES ( 158 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
CpoI DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HhaI HinP1I HindIII KasI KpnI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 10 GTAGAC GT^[AC][GT]AC
AcsI 245 AAATTT [GA]^AATT[CT]
AcyI 480 GGCGTC G[GA]^CG[CT]C
AfaI 452 GTAC GT^AC
AluI 333 AGCT AG^CT
Alw44I 382 GTGCAC G^TGCAC
AlwNI 346 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 382 GTGCAC G^TGCAC
ApoI 245 AAATTT [GA]^AATT[CT]
AspHI 382 GTGCAC G[AT]GC[AT]^C
AvaII 255 GGACC G^G[AT]CC
BbvI 562 GCTGC GCAGC(8/12)
BcgI 309 CGATGAGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 695 CCCGG CC^[GC]GG
BfaI 176 CTAG C^TAG
BglI 726 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 180 AGATCT A^GATCT
BmyI 382 GTGCAC G[AGT]GC[ACT]^C
BsaHI 480 GGCGTC G[GA]^CG[CT]C
BsiHKAI 382 GTGCAC G[AT]GC[AT]^C
BsiYI 362 CCGACAATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 362 CCGACAATCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 101 GTCCC GGGAC(10/14)
Bsp1286I 382 GTGCAC G[AGT]GC[ACT]^C
BspDI 307 ATCGAT AT^CGAT
BspHI 489 TCATGA T^CATGA
BsrDI 152 GCAATG GCAATG(2/0)
BsrI 92 CCAGT ACTGG(1/-1)
BstEII 621 GGTCACC G^GT[ACGT]ACC
BstPI 621 GGTCACC G^GT[ACGT]ACC
BstYI 180 AGATCT [GA]^GATC[CT]
ClaI 307 ATCGAT AT^CGAT
DraI 135 TTTAAA TTT^AAA
DraII 254 AGGACCT [GA]G^G[ACGT]CC[CT]
DraIII 502 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 254 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 621 GGTCACC G^GT[ACGT]ACC
EcoT14I 104 CCAAGG C^C[AT][AT]GG
Fnu4HI 562 GCTGC GC^[ACGT]GC
HapII 696 CCGG C^CGG
Hin1I 480 GGCGTC G[GA]^CG[CT]C
HincII 21 GTTAAC GT[CT]^[GA]AC
HindII 21 GTTAAC GT[CT]^[GA]AC
HpaI 21 GTTAAC GTT^AAC
HpaII 696 CCGG C^CGG
ItaI 562 GCTGC GC^[ACGT]GC
MaeI 176 CTAG C^TAG
MflI 180 AGATCT [GA]^GATC[CT]
MspI 696 CCGG C^CGG
NciI 695 CCCGG CC^[GC]GG
NdeI 656 CATATG CA^TATG
PpuMI 254 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 489 TCATGA T^CATGA
RsaI 452 GTAC GT^AC
SfaNI 691 GATGC GCATC(5/9)
SmlI 163 CTCAAG C^T[CT][GA]AG
SphI 315 GCATGC GCATG^C
StyI 104 CCAAGG C^C[AT][AT]GG
TseI 562 GCTGC G^C[AT]GC
XhoII 180 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BseRI 46 CTCCTC GAGGAG(10/8)
471 GAGGAG
BstF5I 531 GGATG GGATG(2/0)
690 GGATG
BstNI 413 CCAGG CC^[AT]GG
437 CCTGG
Cfr13I 255 GGACC G^G[ACGT]CC
416 GGGCC
DdeI 268 CTGAG C^T[ACGT]AG
469 CTGAG
EcoRII 413 CCAGG ^CC[AT]GG
437 CCTGG
FokI 531 GGATG GGATG(9/13)
690 GGATG
HaeIII 411 GGCC GG^CC
417 GGCC
HgaI 571 GACGC GACGC(5/10)
481 GCGTC
MaeII 506 ACGT A^CGT
520 ACGT
MslI 385 CACAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
642 CACTATGGTG
MvaI 413 CCAGG CC^[AT]GG
437 CCTGG
NspI 315 GCATGC [GA]CATG^[CT]
537 ACATGC
PleI 214 GACTC GAGTC(4/5)
348 GACTC
Sau96I 255 GGACC G^G[ACGT]CC
416 GGGCC
SfcI 147 CTGTAG C^T[GA][CT]AG
457 CTACAG
TaqI 308 TCGA T^CGA
707 TCGA
TfiI 3 GAATC G^A[AT]TC
372 GATTC
Tsp45I 142 GTCAC ^GT[GC]AC
622 GTCAC
Tsp509I 246 AATT ^AATT
423 AATT
TspRI 442 CACTG CA[GC]TG[ACGT][ACGT]^
768 CACTG
TthHB8I 308 TCGA T^CGA
707 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
BsaJI BsmAI BssKI Cac8I DpnII
HinfI HphI MaeIII MboI MboII
MnlI MseI MwoI NdeII NlaIII
Sau3AI ScrFI Tru9I