##################################
# NO CUT ENZYMES (  200 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AluI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspHI       AspI        
AvaI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaJI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsmI        BsoBI       BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BssHII      BssKI       BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstNI       BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HaeIII      HgaI        HhaI        Hin1I       HinP1I      
HindIII     HphI        KasI        KpnI        KspI        
MaeI        MaeIII      MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MspA1I      
MunI        MvaI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
SwaI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AciI          654  CCGC                 CCGC(-3/-1)
AfaI          380  GTAC                 GT^AC
AlwNI         256  CAGCATCTG            CAG[ACGT][ACGT][ACGT]^CTG
BanII         324  GAGCCC               G[GA]GC[CT]^C
BbvI          507  GCAGC                GCAGC(8/12)
BmyI          324  GAGCCC               G[AGT]GC[ACT]^C
BsaWI         190  ACCGGA               [AT]^CCGG[AT]
BseRI         107  GAGGAG               GAGGAG(10/8)
BsmAI         500  GTCTC                GTCTC(1/5)
Bsp1286I      324  GAGCCC               G[AGT]GC[ACT]^C
Bsp1407I      379  TGTACA               T^GTACA
BsrDI         296  GCAATG               GCAATG(2/0)
BsrGI         379  TGTACA               T^GTACA
DpnII          74  GATC                 ^GATC
EarI          579  CTCTTC               CTCTTC(1/4)
Fnu4HI        507  GCAGC                GC^[ACGT]GC
HapII         191  CCGG                 C^CGG
HincII         16  GTTAAC               GT[CT]^[GA]AC
HindII         16  GTTAAC               GT[CT]^[GA]AC
HpaI           16  GTTAAC               GTT^AAC
HpaII         191  CCGG                 C^CGG
ItaI          507  GCAGC                GC^[ACGT]GC
Ksp632I       579  CTCTTC               CTCTTC(1/4)
MaeII          20  ACGT                 A^CGT
MboI           74  GATC                 ^GATC
MspI          191  CCGG                 C^CGG
NdeI          411  CATATG               CA^TATG
NdeII          74  GATC                 ^GATC
PleI          603  GACTC                GAGTC(4/5)
RsaI          380  GTAC                 GT^AC
Sau3AI         74  GATC                 ^GATC
SspBI         379  TGTACA               T^GTACA
TaqI          759  TCGA                 T^CGA
TfiI           99  GATTC                G^A[AT]TC
TseI          507  GCAGC                G^C[AT]GC
TthHB8I       759  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    9 enzymes) #
###################################

Asp700I       434  GAAGTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              518  GAATTCATTC          
BsgI           22  GTGCAG               GTGCAG(16/14)
              126  GTGCAG              
BstF5I        329  CATCC                GGATG(2/0)
              721  GGATG               
FokI          329  CATCC                GGATG(9/13)
              721  GGATG               
HinfI          99  GATTC                G^A[ACGT]TC
              603  GACTC               
MslI          371  CATATAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              667  CACCTTAGTG          
NspI          496  GCATGT               [GA]CATG^[CT]
              544  ACATGC              
SfaNI         258  GCATC                GCATC(5/9)
              510  GCATC               
XmnI          434  GAAGTCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              518  GAATTCATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

AcsI        ApoI        BpmI        BsrI        DdeI        
EcoRI       MboII       MnlI        MseI        NlaIII      
Tru9I       Tsp509I     TspRI