Restriction map for
pF1KB8782
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# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI AhdI AlwI AlwNI
AocI ApaI ApoI AscI AseI
AsnI Asp700I AspEI AvrII BamHI
BbrPI BbvCI BciVI BclI BfaI
BfrI BglI BglII BlnI BpmI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrI BssSI Bst1107I BstBI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI Eam1105I EarI Eco81I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
HindIII HpaI Ksp632I MaeI MamI
MflI MroI MseI NdeI NheI
NruI NsiI NspV PacI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
SbfI ScaI SfiI SfuI SmaI
SnaBI SpeI Sse8387I SspI SwaI
TfiI Tru9I TspRI Van91I XbaI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 106 GGTACC G^GTACC
AflIII 530 ACGCGT A^C[GA][CT]GT
Aor51HI 713 AGCGCT AGC^GCT
Asp718I 106 GGTACC G^GTACC
AspI 151 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 129 GGACC G^G[AT]CC
AviII 348 TGCGCA TGC^GCA
BanII 429 GAGCCC G[GA]GC[CT]^C
BbsI 223 GTCTTC GAAGAC(2/6)
BpuAI 223 GTCTTC GAAGAC(2/6)
BsmAI 8 GAGAC GTCTC(1/5)
BsmFI 809 GGGAC GGGAC(10/14)
Bsp1407I 257 TGTACA T^GTACA
BsrGI 257 TGTACA T^GTACA
BstEII 615 GGTCACC G^GT[ACGT]ACC
BstPI 615 GGTCACC G^GT[ACGT]ACC
Eco47III 713 AGCGCT AGC^GCT
EcoO65I 615 GGTCACC G^GT[ACGT]ACC
FspI 348 TGCGCA TGC^GCA
HincII 157 GTCAAC GT[CT]^[GA]AC
HindII 157 GTCAAC GT[CT]^[GA]AC
KpnI 106 GGTACC GGTAC^C
KspI 770 CCGCGG CCGC^GG
MboII 224 TCTTC GAAGA(8/7)
MfeI 50 CAATTG C^AATTG
MluI 530 ACGCGT A^CGCGT
MspA1I 770 CCGCGG C[AC]G^C[GT]G
MunI 50 CAATTG C^AATTG
NcoI 483 CCATGG C^CATGG
NotI 274 GCGGCCGC GC^GGCCGC
NspI 626 GCATGC [GA]CATG^[CT]
PaeR7I 700 CTCGAG C^TCGAG
PflFI 151 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 742 CTGCAG CTGCA^G
SacII 770 CCGCGG CCGC^GG
SexAI 389 ACCTGGT A^CC[AT]GGT
SfcI 742 CTGCAG C^T[GA][CT]AG
SgrAI 357 CGCCGGCG C[GA]^CCGG[CT]G
SphI 626 GCATGC GCATG^C
SspBI 257 TGTACA T^GTACA
StuI 696 AGGCCT AGG^CCT
Tsp509I 51 AATT ^AATT
Tth111I 151 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 700 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Alw44I 217 GTGCAC G^TGCAC
394 GTGCAC
ApaLI 217 GTGCAC G^TGCAC
394 GTGCAC
AvaI 538 CCCGAG C^[CT]CG[GA]G
700 CTCGAG
BalI 83 TGGCCA TGG^CCA
113 TGGCCA
BbeI 361 GGCGCC GGCGC^C
665 GGCGCC
BlpI 426 GCTGAGC GC^T[ACGT]AGC
518 GCTCAGC
Bpu1102I 426 GCTGAGC GC^T[ACGT]AGC
518 GCTCAGC
BsgI 376 CTGCAC GTGCAG(16/14)
301 GTGCAG
BsiEI 275 CGGCCG CG[GA][CT]^CG
471 CGGCCG
BsiYI 185 CCATCCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
766 CCCTCCGCGGG
BslI 185 CCATCCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
766 CCCTCCGCGGG
BsoBI 538 CCCGAG C^[CT]CG[GA]G
700 CTCGAG
BspMI 287 ACCTGC ACCTGC(4/8)
374 ACCTGC
BstAPI 210 GCACATCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
294 GCAAGCCGTGC
BstXI 116 CCATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
477 CCAGGCCCATGG
CelII 426 GCTGAGC GC^T[ACGT]AGC
518 GCTCAGC
DraII 722 AGGCCCT [GA]G^G[ACGT]CC[CT]
763 AGGCCCT
EagI 275 CGGCCG C^GGCCG
471 CGGCCG
EclXI 275 CGGCCG C^GGCCG
471 CGGCCG
Eco52I 275 CGGCCG C^GGCCG
471 CGGCCG
EcoO109I 722 AGGCCCT [GA]G^G[ACGT]CC[CT]
763 AGGCCCT
EcoT14I 71 CCAAGG C^C[AT][AT]GG
483 CCATGG
HinfI 640 GACTC G^A[ACGT]TC
811 GACTC
KasI 361 GGCGCC G^GCGCC
665 GGCGCC
MaeIII 534 GTCAC ^GT[ACGT]AC
616 GTCAC
MluNI 83 TGGCCA TGG^CCA
113 TGGCCA
MscI 83 TGGCCA TGG^CCA
113 TGGCCA
NaeI 358 GCCGGC GCC^GGC
652 GCCGGC
NarI 361 GGCGCC GG^CGCC
665 GGCGCC
NgoMIV 358 GCCGGC G^CCGGC
652 GCCGGC
PleI 640 GACTC GAGTC(4/5)
811 GACTC
SfaNI 317 GCATC GCATC(5/9)
554 GCATC
SfoI 361 GGCGCC GGC^GCC
665 GGCGCC
SmlI 406 CTCAAG C^T[CT][GA]AG
700 CTCGAG
StyI 71 CCAAGG C^C[AT][AT]GG
483 CCATGG
Tsp45I 534 GTCAC ^GT[GC]AC
616 GTCAC
XcmI 71 CCAAGGTGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
116 CCATCAAGGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI BanI BbvI BcgI BcnI
BmyI BsaHI BsaJI BsiHKAI Bsp1286I
BsrFI BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraIII DsaI EaeI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MboI MnlI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TaqI TseI TthHB8I