################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AlwI 29 GGATC GGATC(4/5) AlwNI 243 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 24 CCTGAGG CC^T[ACGT]AGG ApaI 271 GGGCCC GGGCC^C AvaI 152 CCCGGG C^[CT]CG[GA]G AvaII 15 GGACC G^G[AT]CC BclI 341 TGATCA T^GATCA BpmI 191 CTCCAG CTGGAG(16/14) BsaI 170 GAGACC GGTCTC(1/5) BsmAI 170 GAGAC GTCTC(1/5) BsoBI 152 CCCGGG C^[CT]CG[GA]G BspMI 229 ACCTGC ACCTGC(4/8) BsrBI 18 CCGCTC CCGCTC(-3/-3) Bsu36I 24 CCTGAGG CC^T[ACGT]AGG DraI 817 TTTAAA TTT^AAA Eco81I 24 CCTGAGG CC^T[ACGT]AGG EcoNI 84 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 479 GCGGG CCCGC(4/6) FbaI 341 TGATCA T^GATCA HapII 153 CCGG C^CGG HinfI 103 GATTC G^A[ACGT]TC HpaII 153 CCGG C^CGG MaeIII 127 GTTAC ^GT[ACGT]AC MspI 153 CCGG C^CGG SmaI 152 CCCGGG CCC^GGG SpeI 699 ACTAGT A^CTAGT TaqI 877 TCGA T^CGA TfiI 103 GATTC G^A[AT]TC TthHB8I 877 TCGA T^CGA XbaI 106 TCTAGA T^CTAGA XmaCI 152 CCCGGG C^CCGGG XmaI 152 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanII 196 GGGCTC G[GA]GC[CT]^C 271 GGGCCC BcnI 152 CCCGG CC^[GC]GG 153 CCGGG BmyI 196 GGGCTC G[AGT]GC[ACT]^C 271 GGGCCC BsgI 544 GTGCAG GTGCAG(16/14) 577 GTGCAG Bsp1286I 196 GGGCTC G[AGT]GC[ACT]^C 271 GGGCCC BsrI 49 ACTGG ACTGG(1/-1) 518 CCAGT DpnII 30 GATC ^GATC 342 GATC DraII 270 AGGGCCC [GA]G^G[ACGT]CC[CT] 271 GGGCCCC EcoO109I 270 AGGGCCC [GA]G^G[ACGT]CC[CT] 271 GGGCCCC EcoT14I 84 CCTTGG C^C[AT][AT]GG 209 CCAAGG MboI 30 GATC ^GATC 342 GATC MspA1I 246 CCGCTG C[AC]G^C[GT]G 486 CCGCTG NciI 152 CCCGG CC^[GC]GG 153 CCGGG NdeII 30 GATC ^GATC 342 GATC Sau3AI 30 GATC ^GATC 342 GATC StyI 84 CCTTGG C^C[AT][AT]GG 209 CCAAGG TspRI 580 CAGTG CA[GC]TG[ACGT][ACGT]^ 672 CACTG XcmI 209 CCAAGGAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 368 CCATGAAGCACATGG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AciI AluI BbvI BfaI BsaJI BseRI BsiYI BslI BssKI BstF5I BstNI Cfr13I DdeI EarI EcoRII Fnu4HI FokI HaeIII ItaI Ksp632I MaeI MboII MnlI MseI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI Tru9I TseI Tsp509I