Restriction map for pF1KB8788
##################################
# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        KspI        MaeII       MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        Tsp45I      Tth111I     
Van91I      XhoI        XhoII       XmnI        


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# ONE CUT ENZYMES (   32 enzymes) #
###################################

AlwI           29  GGATC                GGATC(4/5)
AlwNI         243  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           24  CCTGAGG              CC^T[ACGT]AGG
ApaI          271  GGGCCC               GGGCC^C
AvaI          152  CCCGGG               C^[CT]CG[GA]G
AvaII          15  GGACC                G^G[AT]CC
BclI          341  TGATCA               T^GATCA
BpmI          191  CTCCAG               CTGGAG(16/14)
BsaI          170  GAGACC               GGTCTC(1/5)
BsmAI         170  GAGAC                GTCTC(1/5)
BsoBI         152  CCCGGG               C^[CT]CG[GA]G
BspMI         229  ACCTGC               ACCTGC(4/8)
BsrBI          18  CCGCTC               CCGCTC(-3/-3)
Bsu36I         24  CCTGAGG              CC^T[ACGT]AGG
DraI          817  TTTAAA               TTT^AAA
Eco81I         24  CCTGAGG              CC^T[ACGT]AGG
EcoNI          84  CCTTGGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          479  GCGGG                CCCGC(4/6)
FbaI          341  TGATCA               T^GATCA
HapII         153  CCGG                 C^CGG
HinfI         103  GATTC                G^A[ACGT]TC
HpaII         153  CCGG                 C^CGG
MaeIII        127  GTTAC                ^GT[ACGT]AC
MspI          153  CCGG                 C^CGG
SmaI          152  CCCGGG               CCC^GGG
SpeI          699  ACTAGT               A^CTAGT
TaqI          877  TCGA                 T^CGA
TfiI          103  GATTC                G^A[AT]TC
TthHB8I       877  TCGA                 T^CGA
XbaI          106  TCTAGA               T^CTAGA
XmaCI         152  CCCGGG               C^CCGGG
XmaI          152  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

BanII         196  GGGCTC               G[GA]GC[CT]^C
              271  GGGCCC              
BcnI          152  CCCGG                CC^[GC]GG
              153  CCGGG               
BmyI          196  GGGCTC               G[AGT]GC[ACT]^C
              271  GGGCCC              
BsgI          544  GTGCAG               GTGCAG(16/14)
              577  GTGCAG              
Bsp1286I      196  GGGCTC               G[AGT]GC[ACT]^C
              271  GGGCCC              
BsrI           49  ACTGG                ACTGG(1/-1)
              518  CCAGT               
DpnII          30  GATC                 ^GATC
              342  GATC                
DraII         270  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              271  GGGCCCC             
EcoO109I      270  AGGGCCC              [GA]G^G[ACGT]CC[CT]
              271  GGGCCCC             
EcoT14I        84  CCTTGG               C^C[AT][AT]GG
              209  CCAAGG              
MboI           30  GATC                 ^GATC
              342  GATC                
MspA1I        246  CCGCTG               C[AC]G^C[GT]G
              486  CCGCTG              
NciI          152  CCCGG                CC^[GC]GG
              153  CCGGG               
NdeII          30  GATC                 ^GATC
              342  GATC                
Sau3AI         30  GATC                 ^GATC
              342  GATC                
StyI           84  CCTTGG               C^C[AT][AT]GG
              209  CCAAGG              
TspRI         580  CAGTG                CA[GC]TG[ACGT][ACGT]^
              672  CACTG               
XcmI          209  CCAAGGAGAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              368  CCATGAAGCACATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AciI        AluI        BbvI        BfaI        BsaJI       
BseRI       BsiYI       BslI        BssKI       BstF5I      
BstNI       Cfr13I      DdeI        EarI        EcoRII      
Fnu4HI      FokI        HaeIII      ItaI        Ksp632I     
MaeI        MboII       MnlI        MseI        MvaI        
MwoI        NlaIII      NlaIV       Sau96I      ScrFI       
SfaNI       Tru9I       TseI        Tsp509I     
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