Restriction map for pF1KB8791
##################################
# NO CUT ENZYMES (  131 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AhdI        AocI        Aor51HI     
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BamHI       BanI        
BbeI        BbrPI       BbvCI       BciVI       BfaI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
CpoI        DdeI        DraI        DraIII      DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRV       EcoT22I     
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MluI        MroI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        TaqI        TfiI        
Tsp45I      TthHB8I     Van91I      XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AcsI          880  GAATTC               [GA]^AATT[CT]
AflII         145  CTTAAG               C^TTAAG
AgeI          353  ACCGGT               A^CCGGT
Alw44I        279  GTGCAC               G^TGCAC
AlwI          419  GGATC                GGATC(4/5)
AlwNI         745  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          899  GGGCCC               GGGCC^C
ApaLI         279  GTGCAC               G^TGCAC
ApoI          880  GAATTC               [GA]^AATT[CT]
AspI           37  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          950  CCCGGG               C^[CT]CG[GA]G
BanII         899  GGGCCC               G[GA]GC[CT]^C
BcgI          162  CGATTATGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          188  TGATCA               T^GATCA
BfrI          145  CTTAAG               C^TTAAG
BglI          688  GCCAGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         410  AGATCT               A^GATCT
BseRI         106  CTCCTC               GAGGAG(10/8)
BsoBI         950  CCCGGG               C^[CT]CG[GA]G
BsrBI         376  CCGCTC               CCGCTC(-3/-3)
CfoI          533  GCGC                 GCG^C
EcoNI         246  CCTGTCCCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         880  GAATTC               G^AATTC
FbaI          188  TGATCA               T^GATCA
HaeII         532  AGCGCC               [GA]GCGC^[CT]
HgaI          323  GCGTC                GACGC(5/10)
HhaI          533  GCGC                 GCG^C
HinP1I        533  GCGC                 G^CGC
HinfI           3  GAGTC                G^A[ACGT]TC
MaeIII        371  GTTAC                ^GT[ACGT]AC
MfeI          811  CAATTG               C^AATTG
MseI          146  TTAA                 T^TAA
MunI          811  CAATTG               C^AATTG
PflFI          37  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         353  ACCGGT               A^CCGGT
PleI            3  GAGTC                GAGTC(4/5)
PpuMI         437  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         922  CAGCTG               CAG^CTG
SbfI          930  CCTGCAGG             CCTGCA^GG
SmaI          950  CCCGGG               CCC^GGG
SmlI          145  CTTAAG               C^T[CT][GA]AG
Sse8387I      930  CCTGCAGG             CCTGCA^GG
Tru9I         146  TTAA                 T^TAA
TspRI          18  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I        37  GACGGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         950  CCCGGG               C^CCGGG
XmaI          950  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AfaI          256  GTAC                 GT^AC
              315  GTAC                
AflIII        497  ACATGT               A^C[GA][CT]GT
              512  ACATGT              
AspHI         220  GTGCTC               G[AT]GC[AT]^C
              279  GTGCAC              
BalI          119  TGGCCA               TGG^CCA
              362  TGGCCA              
BcnI          950  CCCGG                CC^[GC]GG
              951  CCGGG               
BsaI          635  GAGACC               GGTCTC(1/5)
              708  GGTCTC              
BsiHKAI       220  GTGCTC               G[AT]GC[AT]^C
              279  GTGCAC              
BsmAI         635  GAGAC                GTCTC(1/5)
              709  GTCTC               
BspLU11I      497  ACATGT               A^CATGT
              512  ACATGT              
BspMI         113  ACCTGC               ACCTGC(4/8)
              209  ACCTGC              
BsrFI         353  ACCGGT               [GA]^CCGG[CT]
              905  ACCGGC              
BstYI         410  AGATCT               [GA]^GATC[CT]
              419  GGATCT              
Cfr10I        353  ACCGGT               [GA]^CCGG[CT]
              905  ACCGGC              
DraII         437  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              898  AGGGCCC             
EaeI          119  TGGCCA               [CT]^GGCC[GA]
              362  TGGCCA              
EcoO109I      437  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              898  AGGGCCC             
FauI          375  CCCGC                CCCGC(4/6)
              549  CCCGC               
HincII         43  GTCAAC               GT[CT]^[GA]AC
              835  GTCAAC              
HindII         43  GTCAAC               GT[CT]^[GA]AC
              835  GTCAAC              
MflI          410  AGATCT               [GA]^GATC[CT]
              419  GGATCT              
MluNI         119  TGGCCA               TGG^CCA
              362  TGGCCA              
MscI          119  TGGCCA               TGG^CCA
              362  TGGCCA              
MspA1I        550  CCGCTG               C[AC]G^C[GT]G
              922  CAGCTG              
NciI          950  CCCGG                CC^[GC]GG
              951  CCGGG               
PstI          742  CTGCAG               CTGCA^G
              931  CTGCAG              
RsaI          256  GTAC                 GT^AC
              315  GTAC                
Tsp509I       812  AATT                 ^AATT
              881  AATT                
XhoII         410  AGATCT               [GA]^GATC[CT]
              419  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AluI        AvaII       BbsI        BbvI        
BmyI        BpmI        BpuAI       BsaJI       BsaWI       
BsgI        BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DpnII       DsaI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HpaII       
HphI        ItaI        MboI        MboII       MnlI        
MslI        MspI        MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       NspI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TseI        XcmI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com