################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BfaI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tsp45I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 880 GAATTC [GA]^AATT[CT] AflII 145 CTTAAG C^TTAAG AgeI 353 ACCGGT A^CCGGT Alw44I 279 GTGCAC G^TGCAC AlwI 419 GGATC GGATC(4/5) AlwNI 745 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 899 GGGCCC GGGCC^C ApaLI 279 GTGCAC G^TGCAC ApoI 880 GAATTC [GA]^AATT[CT] AspI 37 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 950 CCCGGG C^[CT]CG[GA]G BanII 899 GGGCCC G[GA]GC[CT]^C BcgI 162 CGATTATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 188 TGATCA T^GATCA BfrI 145 CTTAAG C^TTAAG BglI 688 GCCAGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 410 AGATCT A^GATCT BseRI 106 CTCCTC GAGGAG(10/8) BsoBI 950 CCCGGG C^[CT]CG[GA]G BsrBI 376 CCGCTC CCGCTC(-3/-3) CfoI 533 GCGC GCG^C EcoNI 246 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 880 GAATTC G^AATTC FbaI 188 TGATCA T^GATCA HaeII 532 AGCGCC [GA]GCGC^[CT] HgaI 323 GCGTC GACGC(5/10) HhaI 533 GCGC GCG^C HinP1I 533 GCGC G^CGC HinfI 3 GAGTC G^A[ACGT]TC MaeIII 371 GTTAC ^GT[ACGT]AC MfeI 811 CAATTG C^AATTG MseI 146 TTAA T^TAA MunI 811 CAATTG C^AATTG PflFI 37 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 353 ACCGGT A^CCGGT PleI 3 GAGTC GAGTC(4/5) PpuMI 437 GGGTCCT [GA]G^G[AT]CC[CT] PvuII 922 CAGCTG CAG^CTG SbfI 930 CCTGCAGG CCTGCA^GG SmaI 950 CCCGGG CCC^GGG SmlI 145 CTTAAG C^T[CT][GA]AG Sse8387I 930 CCTGCAGG CCTGCA^GG Tru9I 146 TTAA T^TAA TspRI 18 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 37 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 950 CCCGGG C^CCGGG XmaI 950 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 256 GTAC GT^AC 315 GTAC AflIII 497 ACATGT A^C[GA][CT]GT 512 ACATGT AspHI 220 GTGCTC G[AT]GC[AT]^C 279 GTGCAC BalI 119 TGGCCA TGG^CCA 362 TGGCCA BcnI 950 CCCGG CC^[GC]GG 951 CCGGG BsaI 635 GAGACC GGTCTC(1/5) 708 GGTCTC BsiHKAI 220 GTGCTC G[AT]GC[AT]^C 279 GTGCAC BsmAI 635 GAGAC GTCTC(1/5) 709 GTCTC BspLU11I 497 ACATGT A^CATGT 512 ACATGT BspMI 113 ACCTGC ACCTGC(4/8) 209 ACCTGC BsrFI 353 ACCGGT [GA]^CCGG[CT] 905 ACCGGC BstYI 410 AGATCT [GA]^GATC[CT] 419 GGATCT Cfr10I 353 ACCGGT [GA]^CCGG[CT] 905 ACCGGC DraII 437 GGGTCCT [GA]G^G[ACGT]CC[CT] 898 AGGGCCC EaeI 119 TGGCCA [CT]^GGCC[GA] 362 TGGCCA EcoO109I 437 GGGTCCT [GA]G^G[ACGT]CC[CT] 898 AGGGCCC FauI 375 CCCGC CCCGC(4/6) 549 CCCGC HincII 43 GTCAAC GT[CT]^[GA]AC 835 GTCAAC HindII 43 GTCAAC GT[CT]^[GA]AC 835 GTCAAC MflI 410 AGATCT [GA]^GATC[CT] 419 GGATCT MluNI 119 TGGCCA TGG^CCA 362 TGGCCA MscI 119 TGGCCA TGG^CCA 362 TGGCCA MspA1I 550 CCGCTG C[AC]G^C[GT]G 922 CAGCTG NciI 950 CCCGG CC^[GC]GG 951 CCGGG PstI 742 CTGCAG CTGCA^G 931 CTGCAG RsaI 256 GTAC GT^AC 315 GTAC Tsp509I 812 AATT ^AATT 881 AATT XhoII 410 AGATCT [GA]^GATC[CT] 419 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AvaII BbsI BbvI BmyI BpmI BpuAI BsaJI BsaWI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DpnII DsaI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HpaII HphI ItaI MboI MboII MnlI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI XcmI