##################################
# NO CUT ENZYMES (  209 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwI        AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BssHII      
BssKI       BssSI       Bst1107I    BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr10I      Cfr13I      ClaI        CpoI        DdeI        
DpnII       DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        Fnu4HI      FokI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       ItaI        KasI        KpnI        
Ksp632I     KspI        MamI        MboI        MboII       
MflI        MluI        MluNI       MnlI        MroI        
MscI        MspA1I      MspI        MvaI        MvnI        
NaeI        NarI        NciI        NcoI        NdeII       
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        ScrFI       
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SphI        
Sse8387I    SspI        StyI        SwaI        TseI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   22 enzymes) #
###################################

AciI          297  GCGG                 CCGC(-3/-1)
AfaI          439  GTAC                 GT^AC
AlwNI          35  CAGAGACTG            CAG[ACGT][ACGT][ACGT]^CTG
BfaI          251  CTAG                 C^TAG
Bsp1407I      438  TGTACA               T^GTACA
BsrGI         438  TGTACA               T^GTACA
BstAPI         34  GCAGAGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I         132  GCGTGC               GC[ACGT]^[ACGT]GC
DraI           21  TTTAAA               TTT^AAA
HinfI         228  GATTC                G^A[ACGT]TC
MaeI          251  CTAG                 C^TAG
MaeII         289  ACGT                 A^CGT
MaeIII         47  GTAAC                ^GT[ACGT]AC
MfeI          800  CAATTG               C^AATTG
MunI          800  CAATTG               C^AATTG
MwoI           34  GCAGAGACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII         79  CATG                 CATG^
RsaI          439  GTAC                 GT^AC
SfaNI         485  GCATC                GCATC(5/9)
SpeI          250  ACTAGT               A^CTAGT
SspBI         438  TGTACA               T^GTACA
TfiI          228  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI          791  AGCT                 AG^CT
              809  AGCT                
Alw44I        302  GTGCAC               G^TGCAC
              637  GTGCAC              
ApaLI         302  GTGCAC               G^TGCAC
              637  GTGCAC              
Asp700I       704  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              956  GAAGGCCTTC          
AspHI         302  GTGCAC               G[AT]GC[AT]^C
              637  GTGCAC              
BsiHKAI       302  GTGCAC               G[AT]GC[AT]^C
              637  GTGCAC              
BsmI          184  GAATGC               GAATGC(1/-1)
              540  GCATTC              
BsrDI          13  GCAATG               GCAATG(2/0)
              360  GCAATG              
HaeIII        372  GGCC                 GG^CC
              959  GGCC                
NdeI          179  CATATG               CA^TATG
              682  CATATG              
StuI          371  AGGCCT               AGG^CCT
              958  AGGCCT              
TaqI          156  TCGA                 T^CGA
              315  TCGA                
TthHB8I       156  TCGA                 T^CGA
              315  TCGA                
XmnI          704  GAAGGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              956  GAAGGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

AcsI        ApoI        BmyI        BsmAI       Bsp1286I    
BsrI        EcoRI       HphI        MseI        MslI        
Tru9I       Tsp509I     TspRI