Restriction map for
pF1KB8796
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BamHI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HincII HindII
HindIII HpaI KspI MamI MfeI
MluI MluNI MscI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 545 GGTACC G^GTACC
AccI 559 GTCTAC GT^[AC][GT]AC
AccII 415 CGCG CG^CG
AccIII 767 TCCGGA T^CCGGA
AfaI 546 GTAC GT^AC
AflIII 897 ACATGT A^C[GA][CT]GT
AlwNI 742 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 545 GGTACC G^GTACC
BanII 396 GAGCCC G[GA]GC[CT]^C
BbrPI 540 CACGTG CAC^GTG
BbsI 862 GTCTTC GAAGAC(2/6)
BglI 134 GCCTAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 184 CTGGAG CTGGAG(16/14)
BpuAI 862 GTCTTC GAAGAC(2/6)
BsaWI 767 TCCGGA [AT]^CCGG[AT]
BseAI 767 TCCGGA T^CCGGA
BsmAI 293 GAGAC GTCTC(1/5)
BspEI 767 TCCGGA T^CCGGA
BspHI 365 TCATGA T^CATGA
BspLU11I 897 ACATGT A^CATGT
BsrBI 639 CCGCTC CCGCTC(-3/-3)
BsrFI 923 GCCGGC [GA]^CCGG[CT]
BsrI 275 ACTGG ACTGG(1/-1)
BstEII 444 GGTGACC G^GT[ACGT]ACC
BstPI 444 GGTGACC G^GT[ACGT]ACC
BstUI 415 CGCG CG^CG
Cfr10I 923 GCCGGC [GA]^CCGG[CT]
DraIII 502 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 449 CCATGG C^C[GA][CT]GG
EarI 574 CTCTTC CTCTTC(1/4)
EcoO65I 444 GGTGACC G^GT[ACGT]ACC
EcoT14I 449 CCATGG C^C[AT][AT]GG
KpnI 545 GGTACC GGTAC^C
Ksp632I 574 CTCTTC CTCTTC(1/4)
MaeIII 445 GTGAC ^GT[ACGT]AC
MroI 767 TCCGGA T^CCGGA
MseI 845 TTAA T^TAA
MvnI 415 CGCG CG^CG
NaeI 923 GCCGGC GCC^GGC
NcoI 449 CCATGG C^CATGG
NgoMIV 923 GCCGGC G^CCGGC
NspI 897 ACATGT [GA]CATG^[CT]
PmaCI 540 CACGTG CAC^GTG
PmlI 540 CACGTG CAC^GTG
PvuII 708 CAGCTG CAG^CTG
RcaI 365 TCATGA T^CATGA
RsaI 546 GTAC GT^AC
SexAI 230 ACCTGGT A^CC[AT]GGT
StuI 74 AGGCCT AGG^CCT
StyI 449 CCATGG C^C[AT][AT]GG
Tru9I 845 TTAA T^TAA
Tsp45I 445 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcyI 656 GGCGCC G[GA]^CG[CT]C
985 GGCGCC
AocI 8 CCTCAGG CC^T[ACGT]AGG
624 CCTCAGG
AspHI 24 GAGCAC G[AT]GC[AT]^C
853 GTGCTC
BbeI 656 GGCGCC GGCGC^C
985 GGCGCC
BbvCI 129 CCTCAGC CCTCAGC(-5/-2)
705 CCTCAGC
BcnI 435 CCGGG CC^[GC]GG
849 CCCGG
BfaI 136 CTAG C^TAG
793 CTAG
BsaAI 540 CACGTG [CT]AC^GT[GA]
613 TACGTG
BsaHI 656 GGCGCC G[GA]^CG[CT]C
985 GGCGCC
BseRI 127 CTCCTC GAGGAG(10/8)
310 CTCCTC
BsiHKAI 24 GAGCAC G[AT]GC[AT]^C
853 GTGCTC
BstYI 260 GGATCT [GA]^GATC[CT]
620 AGATCC
Bsu36I 8 CCTCAGG CC^T[ACGT]AGG
624 CCTCAGG
Eco81I 8 CCTCAGG CC^T[ACGT]AGG
624 CCTCAGG
HaeII 656 GGCGCC [GA]GCGC^[CT]
985 GGCGCC
HgaI 744 GACGC GACGC(5/10)
416 GCGTC
Hin1I 656 GGCGCC G[GA]^CG[CT]C
985 GGCGCC
HinfI 4 GAGTC G^A[ACGT]TC
187 GAGTC
KasI 656 GGCGCC G^GCGCC
985 GGCGCC
MaeI 136 CTAG C^TAG
793 CTAG
MaeII 541 ACGT A^CGT
614 ACGT
MflI 260 GGATCT [GA]^GATC[CT]
620 AGATCC
MslI 450 CATGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
687 CATCGTGGTG
MspA1I 429 CCGCTG C[AC]G^C[GT]G
708 CAGCTG
NarI 656 GGCGCC GG^CGCC
985 GGCGCC
NciI 435 CCGGG CC^[GC]GG
849 CCCGG
PleI 4 GAGTC GAGTC(4/5)
187 GAGTC
PstI 739 CTGCAG CTGCA^G
928 CTGCAG
SfaNI 347 GCATC GCATC(5/9)
470 GCATC
SfoI 656 GGCGCC GGC^GCC
985 GGCGCC
TaqI 488 TCGA T^CGA
949 TCGA
TthHB8I 488 TCGA T^CGA
949 TCGA
XhoII 260 GGATCT [GA]^GATC[CT]
620 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AvaII BanI
BbvI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV PpuMI Sau3AI Sau96I
ScrFI SfcI TseI TspRI