################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssKI BssSI BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MflI MluI MroI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScrFI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI TaqI TseI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 514 GTATAC GT^[AC][GT]AC AciI 348 CCGC CCGC(-3/-1) AcsI 433 AAATTT [GA]^AATT[CT] AflIII 16 ACATGT A^C[GA][CT]GT ApoI 433 AAATTT [GA]^AATT[CT] Asp700I 228 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 417 TGGCCA TGG^CCA BbsI 951 GAAGAC GAAGAC(2/6) BpuAI 951 GAAGAC GAAGAC(2/6) BsaI 607 GGTCTC GGTCTC(1/5) BsaJI 44 CCATGG C^C[ACGT][ACGT]GG BsgI 192 GTGCAG GTGCAG(16/14) BspHI 209 TCATGA T^CATGA BspLU11I 16 ACATGT A^CATGT BspMI 604 GCAGGT ACCTGC(4/8) Bst1107I 514 GTATAC GTA^TAC BstZ17I 514 GTATAC GTA^TAC DraI 802 TTTAAA TTT^AAA DraIII 166 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 44 CCATGG C^C[GA][CT]GG EcoT14I 44 CCATGG C^C[AT][AT]GG Fnu4HI 347 GCCGC GC^[ACGT]GC HphI 881 TCACC GGTGA(8/7) ItaI 347 GCCGC GC^[ACGT]GC MaeII 703 ACGT A^CGT MfeI 847 CAATTG C^AATTG MluNI 417 TGGCCA TGG^CCA MscI 417 TGGCCA TGG^CCA MunI 847 CAATTG C^AATTG NcoI 44 CCATGG C^CATGG RcaI 209 TCATGA T^CATGA ScaI 648 AGTACT AGT^ACT SfcI 536 CTACAG C^T[GA][CT]AG SphI 297 GCATGC GCATG^C SspI 408 AATATT AAT^ATT StyI 44 CCATGG C^C[AT][AT]GG TspRI 195 CAGTG CA[GC]TG[ACGT][ACGT]^ XmnI 228 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BfaI 455 CTAG C^TAG 580 CTAG BsrI 489 ACTGG ACTGG(1/-1) 505 CCAGT BstF5I 66 CATCC GGATG(2/0) 759 CATCC Cac8I 297 GCATGC GC[ACGT]^[ACGT]GC 440 GCCAGC DpnII 36 GATC ^GATC 591 GATC EaeI 417 TGGCCA [CT]^GGCC[GA] 896 CGGCCA EarI 39 CTCTTC CTCTTC(1/4) 547 GAAGAG EcoT22I 295 ATGCAT ATGCA^T 465 ATGCAT FokI 66 CATCC GGATG(9/13) 759 CATCC HaeIII 418 GGCC GG^CC 897 GGCC HinfI 373 GATTC G^A[ACGT]TC 765 GATTC Ksp632I 39 CTCTTC CTCTTC(1/4) 547 GAAGAG MaeI 455 CTAG C^TAG 580 CTAG MboI 36 GATC ^GATC 591 GATC NdeII 36 GATC ^GATC 591 GATC NlaIV 583 GGAGCC GG[ACGT]^[ACGT]CC 616 GGAACC NsiI 295 ATGCAT ATGCA^T 465 ATGCAT NspI 16 ACATGT [GA]CATG^[CT] 297 GCATGC Sau3AI 36 GATC ^GATC 591 GATC TfiI 373 GATTC G^A[AT]TC 765 GATTC Tsp45I 162 GTCAC ^GT[GC]AC 172 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AfaI BsmAI DdeI MaeIII MboII MnlI MseI NdeI NlaIII RsaI Tru9I Tsp509I