Restriction map for
pF1KB8800
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstUI
BstXI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraI DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoRI EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NlaIV
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AciI 759 CCGC CCGC(-3/-1)
AfaI 111 GTAC GT^AC
AflIII 341 ACACGT A^C[GA][CT]GT
AlwI 375 GGATC GGATC(4/5)
AlwNI 187 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 834 CCTGAGG CC^T[ACGT]AGG
AspHI 856 GTGCTC G[AT]GC[AT]^C
BalI 291 TGGCCA TGG^CCA
BbsI 22 GAAGAC GAAGAC(2/6)
BglII 682 AGATCT A^GATCT
BmyI 856 GTGCTC G[AGT]GC[ACT]^C
BpmI 662 CTGGAG CTGGAG(16/14)
BpuAI 22 GAAGAC GAAGAC(2/6)
BsaI 939 GGTCTC GGTCTC(1/5)
BseRI 61 GAGGAG GAGGAG(10/8)
BsgI 243 CTGCAC GTGCAG(16/14)
BsiHKAI 856 GTGCTC G[AT]GC[AT]^C
BsmAI 940 GTCTC GTCTC(1/5)
Bsp1286I 856 GTGCTC G[AGT]GC[ACT]^C
BspHI 746 TCATGA T^CATGA
BspMI 361 ACCTGC ACCTGC(4/8)
BsrDI 509 GCAATG GCAATG(2/0)
BstBI 884 TTCGAA TT^CGAA
BstYI 682 AGATCT [GA]^GATC[CT]
Bsu36I 834 CCTGAGG CC^T[ACGT]AGG
DraII 838 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 994 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 291 TGGCCA [CT]^GGCC[GA]
Eco81I 834 CCTGAGG CC^T[ACGT]AGG
EcoO109I 838 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRV 471 GATATC GAT^ATC
MaeII 343 ACGT A^CGT
MflI 682 AGATCT [GA]^GATC[CT]
MluNI 291 TGGCCA TGG^CCA
MscI 291 TGGCCA TGG^CCA
NdeI 534 CATATG CA^TATG
NspV 884 TTCGAA TT^CGAA
PflMI 993 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 79 CTGCAG CTGCA^G
RcaI 746 TCATGA T^CATGA
RsaI 111 GTAC GT^AC
SfcI 79 CTGCAG C^T[GA][CT]AG
SfiI 399 GGCCACCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 884 TTCGAA TT^CGAA
StuI 407 AGGCCT AGG^CCT
Van91I 993 CCACAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 682 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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BbvI 53 GCAGC GCAGC(8/12)
761 GCAGC
BglI 311 GCCTACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
400 GCCACCAAGGC
BstEII 447 GGTCACC G^GT[ACGT]ACC
541 GGTTACC
BstPI 447 GGTCACC G^GT[ACGT]ACC
541 GGTTACC
Cfr13I 260 GGGCC G^G[ACGT]CC
839 GGGCC
DdeI 835 CTGAG C^T[ACGT]AG
962 CTGAG
EcoO65I 447 GGTCACC G^GT[ACGT]ACC
541 GGTTACC
EcoT14I 404 CCAAGG C^C[AT][AT]GG
933 CCTTGG
Fnu4HI 53 GCAGC GC^[ACGT]GC
761 GCAGC
ItaI 53 GCAGC GC^[ACGT]GC
761 GCAGC
MwoI 311 GCCTACTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
400 GCCACCAAGGC
NspI 107 GCATGT [GA]CATG^[CT]
1013 GCATGT
Sau96I 260 GGGCC G^G[ACGT]CC
839 GGGCC
SexAI 113 ACCTGGT A^CC[AT]GGT
230 ACCTGGT
StyI 404 CCAAGG C^C[AT][AT]GG
933 CCTTGG
TaqI 524 TCGA T^CGA
885 TCGA
TseI 53 GCAGC G^C[AT]GC
761 GCAGC
Tsp45I 448 GTCAC ^GT[GC]AC
814 GTGAC
TthHB8I 524 TCGA T^CGA
885 TCGA
XcmI 18 CCATGAAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
495 CCAAATTATCTATGG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
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AluI BsaJI BsiYI BslI BsrI
BssKI BstF5I BstNI Cac8I DpnII
EcoRII FokI HaeIII HphI MaeIII
MboI MboII MnlI MseI MslI
MvaI NdeII NlaIII Sau3AI ScrFI
SfaNI Tru9I Tsp509I TspRI