Restriction map for
pF1KB8811
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
KspI MaeI MaeIII MamI MfeI
MluI MroI MseI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tru9I Tsp45I Tsp509I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 160 GTCTAC GT^[AC][GT]AC
AflIII 11 ACACGT A^C[GA][CT]GT
AvaI 259 CCCGGG C^[CT]CG[GA]G
BamHI 248 GGATCC G^GATCC
BglI 442 GCCAACTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 967 CTGGAG CTGGAG(16/14)
BseRI 781 GAGGAG GAGGAG(10/8)
BsoBI 259 CCCGGG C^[CT]CG[GA]G
Bsp1407I 194 TGTACA T^GTACA
BsrGI 194 TGTACA T^GTACA
BstAPI 903 GCACAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 248 GGATCC [GA]^GATC[CT]
EcoNI 983 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 865 GTCAAC GT[CT]^[GA]AC
HindII 865 GTCAAC GT[CT]^[GA]AC
HinfI 34 GACTC G^A[ACGT]TC
MflI 248 GGATCC [GA]^GATC[CT]
NcoI 518 CCATGG C^CATGG
PflMI 939 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 34 GACTC GAGTC(4/5)
PpuMI 262 GGGTCCT [GA]G^G[AT]CC[CT]
SapI 471 GCTCTTC GCTCTTC(1/4)
SexAI 1052 ACCAGGT A^CC[AT]GGT
SmaI 259 CCCGGG CCC^GGG
SspBI 194 TGTACA T^GTACA
TaqI 779 TCGA T^CGA
TspRI 812 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 779 TCGA T^CGA
Van91I 939 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 98 CCACCAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 248 GGATCC [GA]^GATC[CT]
XmaCI 259 CCCGGG C^CCGGG
XmaI 259 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 195 GTAC GT^AC
600 GTAC
AlwI 249 GATCC GGATC(4/5)
248 GGATC
BanII 411 GAGCTC G[GA]GC[CT]^C
469 GAGCTC
BbvCI 174 CCTCAGC CCTCAGC(-5/-2)
678 CCTCAGC
BsaI 479 GAGACC GGTCTC(1/5)
970 GAGACC
BsmAI 479 GAGAC GTCTC(1/5)
970 GAGAC
BspMI 188 ACCTGC ACCTGC(4/8)
908 ACCTGC
DdeI 175 CTCAG C^T[ACGT]AG
679 CTCAG
DpnII 249 GATC ^GATC
663 GATC
DraII 262 GGGTCCT [GA]G^G[ACGT]CC[CT]
896 AGGCCCT
EarI 472 CTCTTC CTCTTC(1/4)
571 CTCTTC
EcoO109I 262 GGGTCCT [GA]G^G[ACGT]CC[CT]
896 AGGCCCT
EcoT14I 518 CCATGG C^C[AT][AT]GG
893 CCAAGG
HapII 260 CCGG C^CGG
603 CCGG
HpaII 260 CCGG C^CGG
603 CCGG
Ksp632I 472 CTCTTC CTCTTC(1/4)
571 CTCTTC
MboI 249 GATC ^GATC
663 GATC
MslI 81 CATCGGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
330 CATCTCGGTG
MspI 260 CCGG C^CGG
603 CCGG
NdeII 249 GATC ^GATC
663 GATC
PvuII 1020 CAGCTG CAG^CTG
1060 CAGCTG
RsaI 195 GTAC GT^AC
600 GTAC
SacI 411 GAGCTC GAGCT^C
469 GAGCTC
Sau3AI 249 GATC ^GATC
663 GATC
StyI 518 CCATGG C^C[AT][AT]GG
893 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaII BalI BanI BbeI BbrPI
BbvI BcnI BmyI BsaAI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HphI ItaI
KasI MaeII MboII MluNI MnlI
MscI MspA1I MvaI MvnI MwoI
NarI NciI NlaIII NlaIV PmaCI
PmlI Sau96I ScrFI SfaNI SfoI
TseI