Restriction map for
pF1KB8817
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvrII BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FseI HaeII HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeII MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 229 AAATTC [GA]^AATT[CT]
AlwI 213 GATCC GGATC(4/5)
ApoI 229 AAATTC [GA]^AATT[CT]
AspHI 945 GTGCTC G[AT]GC[AT]^C
AviII 242 TGCGCA TGC^GCA
BalI 548 TGGCCA TGG^CCA
BanI 375 GGTGCC G^G[CT][GA]CC
BciVI 406 GTATCC GTATCC(6/5)
BclI 224 TGATCA T^GATCA
BsaBI 573 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 628 GAGGAG GAGGAG(10/8)
BsgI 14 GTGCAG GTGCAG(16/14)
BsiHKAI 945 GTGCTC G[AT]GC[AT]^C
BsrDI 264 CATTGC GCAATG(2/0)
BsrFI 440 ACCGGC [GA]^CCGG[CT]
BsrI 93 ACTGG ACTGG(1/-1)
BstXI 800 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 243 GCGC GCG^C
Cfr10I 440 ACCGGC [GA]^CCGG[CT]
DrdI 880 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 548 TGGCCA [CT]^GGCC[GA]
EarI 633 GAAGAG CTCTTC(1/4)
EcoT14I 206 CCTTGG C^C[AT][AT]GG
FbaI 224 TGATCA T^GATCA
FspI 242 TGCGCA TGC^GCA
HhaI 243 GCGC GCG^C
HinP1I 243 GCGC G^CGC
HincII 871 GTTGAC GT[CT]^[GA]AC
HindII 871 GTTGAC GT[CT]^[GA]AC
Ksp632I 633 GAAGAG CTCTTC(1/4)
MamI 573 GATTGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 548 TGGCCA TGG^CCA
MscI 548 TGGCCA TGG^CCA
MseI 334 TTAA T^TAA
MslI 798 CACCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 239 GCATGC [GA]CATG^[CT]
PleI 1117 GACTC GAGTC(4/5)
PvuII 837 CAGCTG CAG^CTG
SapI 633 GAAGAGC GCTCTTC(1/4)
SexAI 371 ACCTGGT A^CC[AT]GGT
SphI 239 GCATGC GCATG^C
StyI 206 CCTTGG C^C[AT][AT]GG
TfiI 488 GAATC G^A[AT]TC
Tru9I 334 TTAA T^TAA
Tsp45I 1063 GTGAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 308 GTCTAC GT^[AC][GT]AC
457 GTCTAC
BcnI 558 CCGGG CC^[GC]GG
585 CCCGG
BfaI 83 CTAG C^TAG
466 CTAG
BglI 268 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
529 GCCCTGCTGGC
BglII 285 AGATCT A^GATCT
476 AGATCT
BpmI 755 CTGGAG CTGGAG(16/14)
1087 CTGGAG
BsmFI 97 GTCCC GGGAC(10/14)
542 GTCCC
BstYI 285 AGATCT [GA]^GATC[CT]
476 AGATCT
DdeI 107 CTGAG C^T[ACGT]AG
1052 CTGAG
DraIII 300 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
370 CACCTGGTG
HinfI 488 GAATC G^A[ACGT]TC
1117 GACTC
MaeI 83 CTAG C^TAG
466 CTAG
MaeIII 250 GTAAC ^GT[ACGT]AC
1063 GTGAC
MflI 285 AGATCT [GA]^GATC[CT]
476 AGATCT
NciI 558 CCGGG CC^[GC]GG
585 CCCGG
PpuMI 354 GGGTCCT [GA]G^G[AT]CC[CT]
887 AGGTCCT
SfcI 654 CTATAG C^T[GA][CT]AG
993 CTACAG
XhoII 285 AGATCT [GA]^GATC[CT]
476 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BbvI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MboI MboII MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PflMI RsaI Sau3AI
Sau96I ScrFI SfaNI TseI Tsp509I
TspRI Van91I XcmI