Restriction map for
pF1KB8819
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI AluI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BsaHI BseAI
BseRI BsgI BsiEI BsiWI BsmFI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstYI BstZ17I
Bsu36I CelII Cfr10I CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HindIII KasI KpnI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 207 CGCG CG^CG
AfaI 60 GTAC GT^AC
AflII 297 CTTAAG C^TTAAG
AlwI 154 GATCC GGATC(4/5)
ApaI 359 GGGCCC GGGCC^C
AseI 250 ATTAAT AT^TAAT
AsnI 250 ATTAAT AT^TAAT
AspHI 818 GAGCAC G[AT]GC[AT]^C
AvaII 75 GGACC G^G[AT]CC
BanI 903 GGTGCC G^G[CT][GA]CC
BfaI 769 CTAG C^TAG
BfrI 297 CTTAAG C^TTAAG
BpmI 308 CTGGAG CTGGAG(16/14)
BsaAI 61 TACGTG [CT]AC^GT[GA]
BsaI 278 GGTCTC GGTCTC(1/5)
BsaWI 156 TCCGGT [AT]^CCGG[AT]
BsiHKAI 818 GAGCAC G[AT]GC[AT]^C
BsmBI 620 GAGACG CGTCTC(1/5)
BspDI 790 ATCGAT AT^CGAT
BspMI 1139 GCAGGT ACCTGC(4/8)
BsrI 701 CCAGT ACTGG(1/-1)
BssSI 745 CACGAG CACGAG(-5/-1)
BstEII 159 GGTGACC G^GT[ACGT]ACC
BstPI 159 GGTGACC G^GT[ACGT]ACC
BstUI 207 CGCG CG^CG
BstXI 1064 CCAAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1137 GCGC GCG^C
ClaI 790 ATCGAT AT^CGAT
DpnII 154 GATC ^GATC
DrdI 76 GACCCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 206 CCGCGG C^C[GA][CT]GG
EcoNI 1122 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 159 GGTGACC G^GT[ACGT]ACC
HapII 157 CCGG C^CGG
HhaI 1137 GCGC GCG^C
HinP1I 1137 GCGC G^CGC
HincII 190 GTTAAC GT[CT]^[GA]AC
HindII 190 GTTAAC GT[CT]^[GA]AC
HpaI 190 GTTAAC GTT^AAC
HpaII 157 CCGG C^CGG
KspI 206 CCGCGG CCGC^GG
MaeI 769 CTAG C^TAG
MaeII 62 ACGT A^CGT
MboI 154 GATC ^GATC
MslI 490 CACATGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 157 CCGG C^CGG
MvnI 207 CGCG CG^CG
NdeII 154 GATC ^GATC
PshBI 250 ATTAAT AT^TAAT
RsaI 60 GTAC GT^AC
SacII 206 CCGCGG CCGC^GG
SapI 550 GAAGAGC GCTCTTC(1/4)
Sau3AI 154 GATC ^GATC
StuI 115 AGGCCT AGG^CCT
TaqI 791 TCGA T^CGA
TthHB8I 791 TCGA T^CGA
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# TWO CUT ENZYMES ( 14 enzymes) #
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BanII 359 GGGCCC G[GA]GC[CT]^C
1098 GGGCTC
BbvCI 562 GCTGAGG CCTCAGC(-5/-2)
870 GCTGAGG
BglI 170 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
754 GCCCAAAAGGC
DraII 358 AGGGCCC [GA]G^G[ACGT]CC[CT]
359 GGGCCCT
EaeI 464 TGGCCG [CT]^GGCC[GA]
1086 TGGCCG
EarI 550 GAAGAG CTCTTC(1/4)
1054 GAAGAG
EcoO109I 358 AGGGCCC [GA]G^G[ACGT]CC[CT]
359 GGGCCCT
Ksp632I 550 GAAGAG CTCTTC(1/4)
1054 GAAGAG
MspA1I 206 CCGCGG C[AC]G^C[GT]G
467 CCGCTG
NspI 95 ACATGC [GA]CATG^[CT]
1113 GCATGT
SfaNI 211 GATGC GCATC(5/9)
697 GATGC
SmlI 46 CTCAAG C^T[CT][GA]AG
297 CTTAAG
TspRI 702 CAGTG CA[GC]TG[ACGT][ACGT]^
1070 CAGTG
XcmI 519 CCAGGTGTATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
992 CCAAAGTGATGATGG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI ApoI BbvI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrBI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EcoRII FauI Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboII MnlI MseI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TfiI Tru9I TseI Tsp45I Tsp509I