Restriction map for
pF1KB8825
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstYI BstZ17I ClaI CpoI DpnII
DraI DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MboI MfeI MflI
MluI MroI MseI MunI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI Sau3AI
SbfI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1027 GTAGAC GT^[AC][GT]AC
AocI 1049 CCTCAGG CC^T[ACGT]AGG
ApaI 628 GGGCCC GGGCC^C
AvaI 1236 CTCGGG C^[CT]CG[GA]G
BalI 1034 TGGCCA TGG^CCA
BbeI 887 GGCGCC GGCGC^C
BfaI 424 CTAG C^TAG
BlpI 375 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 375 GCTGAGC GC^T[ACGT]AGC
BsaI 1216 GAGACC GGTCTC(1/5)
BsiEI 294 CGACCG CG[GA][CT]^CG
BsmAI 1216 GAGAC GTCTC(1/5)
BsmFI 37 GGGAC GGGAC(10/14)
BsoBI 1236 CTCGGG C^[CT]CG[GA]G
BsrFI 380 GCCGGC [GA]^CCGG[CT]
BssSI 246 CTCGTG CACGAG(-5/-1)
BstEII 1044 GGTCACC G^GT[ACGT]ACC
BstPI 1044 GGTCACC G^GT[ACGT]ACC
BstXI 899 CCATGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1049 CCTCAGG CC^T[ACGT]AGG
CelII 375 GCTGAGC GC^T[ACGT]AGC
Cfr10I 380 GCCGGC [GA]^CCGG[CT]
DrdI 475 GACGCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1049 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1044 GGTCACC G^GT[ACGT]ACC
KasI 887 GGCGCC G^GCGCC
MaeI 424 CTAG C^TAG
MaeII 1031 ACGT A^CGT
MluNI 1034 TGGCCA TGG^CCA
MscI 1034 TGGCCA TGG^CCA
MslI 975 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 380 GCCGGC GCC^GGC
NarI 887 GGCGCC GG^CGCC
NgoMIV 380 GCCGGC G^CCGGC
NspI 1064 ACATGC [GA]CATG^[CT]
PflMI 849 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 793 CTGCAG CTGCA^G
PvuII 796 CAGCTG CAG^CTG
SapI 212 GCTCTTC GCTCTTC(1/4)
SexAI 956 ACCTGGT A^CC[AT]GGT
SfoI 887 GGCGCC GGC^GCC
TaqI 293 TCGA T^CGA
TfiI 1201 GATTC G^A[AT]TC
TthHB8I 293 TCGA T^CGA
Van91I 849 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 357 CGCG CG^CG
640 CGCG
AcyI 178 GACGCC G[GA]^CG[CT]C
887 GGCGCC
AfaI 15 GTAC GT^AC
254 GTAC
AvaII 944 GGACC G^G[AT]CC
984 GGACC
BanI 523 GGTGCC G^G[CT][GA]CC
887 GGCGCC
BbsI 26 GAAGAC GAAGAC(2/6)
1188 GTCTTC
BpmI 1171 CTCCAG CTGGAG(16/14)
199 CTGGAG
BpuAI 26 GAAGAC GAAGAC(2/6)
1188 GTCTTC
BsaHI 178 GACGCC G[GA]^CG[CT]C
887 GGCGCC
BseRI 325 CTCCTC GAGGAG(10/8)
50 GAGGAG
BspMI 652 ACCTGC ACCTGC(4/8)
520 GCAGGT
BsrBI 460 CCGCTC CCGCTC(-3/-3)
1120 GAGCGG
BsrI 30 ACTGG ACTGG(1/-1)
486 CCAGT
BstUI 357 CGCG CG^CG
640 CGCG
DraII 627 GGGGCCC [GA]G^G[ACGT]CC[CT]
628 GGGCCCC
DraIII 163 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
955 CACCTGGTG
EarI 213 CTCTTC CTCTTC(1/4)
529 CTCTTC
EcoO109I 627 GGGGCCC [GA]G^G[ACGT]CC[CT]
628 GGGCCCC
HgaI 178 GACGC GACGC(5/10)
475 GACGC
Hin1I 178 GACGCC G[GA]^CG[CT]C
887 GGCGCC
Ksp632I 213 CTCTTC CTCTTC(1/4)
529 CTCTTC
MvnI 357 CGCG CG^CG
640 CGCG
PleI 85 GACTC GAGTC(4/5)
244 GACTC
RsaI 15 GTAC GT^AC
254 GTAC
SfaNI 1131 GATGC GCATC(5/9)
572 GCATC
XcmI 486 CCAGTGGGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
899 CCATGCGGCTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI EaeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboII MnlI MspA1I
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI SfcI StuI
TseI Tsp45I TspRI