Restriction map for
pF1KB8828
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AvrII BamHI BbeI BbrPI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspEI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FbaI
FseI HaeII HgaI HincII HindII
HindIII HpaI KasI Ksp632I KspI
MamI MflI MluI MroI NarI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 554 GACGTC GACGT^C
Acc65I 28 GGTACC G^GTACC
AccII 695 CGCG CG^CG
AcyI 554 GACGTC G[GA]^CG[CT]C
Asp718I 28 GGTACC G^GTACC
AspI 551 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 500 CTCGGG C^[CT]CG[GA]G
AviII 1280 TGCGCA TGC^GCA
BalI 785 TGGCCA TGG^CCA
BbsI 318 GAAGAC GAAGAC(2/6)
BbvCI 342 GCTGAGG CCTCAGC(-5/-2)
BcgI 631 CGAGGTGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 718 CTAG C^TAG
BpuAI 318 GAAGAC GAAGAC(2/6)
BsaHI 554 GACGTC G[GA]^CG[CT]C
BsmI 729 GAATGC GAATGC(1/-1)
BsoBI 500 CTCGGG C^[CT]CG[GA]G
BspDI 1197 ATCGAT AT^CGAT
BspHI 558 TCATGA T^CATGA
BsrDI 924 CATTGC GCAATG(2/0)
BstUI 695 CGCG CG^CG
BstXI 1241 CCACTCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1281 GCGC GCG^C
ClaI 1197 ATCGAT AT^CGAT
FspI 1280 TGCGCA TGC^GCA
HhaI 1281 GCGC GCG^C
Hin1I 554 GACGTC G[GA]^CG[CT]C
HinP1I 1281 GCGC G^CGC
KpnI 28 GGTACC GGTAC^C
MaeI 718 CTAG C^TAG
MaeII 555 ACGT A^CGT
MfeI 1115 CAATTG C^AATTG
MluNI 785 TGGCCA TGG^CCA
MscI 785 TGGCCA TGG^CCA
MunI 1115 CAATTG C^AATTG
MvnI 695 CGCG CG^CG
NaeI 626 GCCGGC GCC^GGC
NdeI 671 CATATG CA^TATG
NgoMIV 626 GCCGGC G^CCGGC
NspI 1277 GCATGC [GA]CATG^[CT]
PflFI 551 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 407 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 558 TCATGA T^CATGA
SgrAI 625 CGCCGGCG C[GA]^CCGG[CT]G
SphI 1277 GCATGC GCATG^C
TaqI 1198 TCGA T^CGA
TfiI 1220 GAATC G^A[AT]TC
Tth111I 551 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1198 TCGA T^CGA
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# TWO CUT ENZYMES ( 34 enzymes) #
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AfaI 29 GTAC GT^AC
799 GTAC
AlwNI 866 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
877 CAGACTCTG
AspHI 852 GAGCTC G[AT]GC[AT]^C
1007 GAGCTC
AvaII 408 GGTCC G^G[AT]CC
603 GGACC
BanI 28 GGTACC G^G[CT][GA]CC
1131 GGCACC
BcnI 9 CCGGG CC^[GC]GG
32 CCGGG
BglI 76 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1183 GCCCCTGTGGC
BsiHKAI 852 GAGCTC G[AT]GC[AT]^C
1007 GAGCTC
BsmAI 128 GAGAC GTCTC(1/5)
1144 GTCTC
BsrFI 378 ACCGGC [GA]^CCGG[CT]
626 GCCGGC
BstAPI 158 GCAAGCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1036 GCACCTCCTGC
Cfr10I 378 ACCGGC [GA]^CCGG[CT]
626 GCCGGC
DpnII 562 GATC ^GATC
1083 GATC
DraII 48 AGGCCCT [GA]G^G[ACGT]CC[CT]
407 AGGTCCC
EaeI 785 TGGCCA [CT]^GGCC[GA]
1289 CGGCCA
EcoO109I 48 AGGCCCT [GA]G^G[ACGT]CC[CT]
407 AGGTCCC
EcoRV 170 GATATC GAT^ATC
571 GATATC
FauI 101 GCGGG CCCGC(4/6)
747 GCGGG
MboI 562 GATC ^GATC
1083 GATC
MboII 318 GAAGA GAAGA(8/7)
230 TCTTC
MseI 760 TTAA T^TAA
914 TTAA
MslI 1242 CACTCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1287 CACGGCCATG
NciI 9 CCGGG CC^[GC]GG
32 CCGGG
NdeII 562 GATC ^GATC
1083 GATC
PflMI 1122 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1206 CCAACCCTTGG
RsaI 29 GTAC GT^AC
799 GTAC
SacI 852 GAGCTC GAGCT^C
1007 GAGCTC
Sau3AI 562 GATC ^GATC
1083 GATC
SfcI 123 CTGTAG C^T[GA][CT]AG
961 CTACAG
SmlI 135 CTTGAG C^T[CT][GA]AG
430 CTCAAG
Tru9I 760 TTAA T^TAA
914 TTAA
Tsp509I 271 AATT ^AATT
1116 AATT
Van91I 1122 CCAGGGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1206 CCAACCCTTGG
XcmI 1148 CCATGGCTCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1238 CCACCACTCCCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DsaI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MnlI MspA1I
MspI MvaI MwoI NcoI NlaIII
NlaIV PleI PvuII Sau96I ScrFI
SfaNI StyI TseI Tsp45I TspRI