Restriction map for
pF1KB8837
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvrII BamHI BbrPI
BbsI BciVI BclI BfaI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaWI BseAI BsgI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfuI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI Tsp509I Tth111I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 22 enzymes) #
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AflII 1342 CTTAAG C^TTAAG
AspHI 697 GAGCAC G[AT]GC[AT]^C
BanII 1278 GGGCTC G[GA]GC[CT]^C
BbvCI 1177 GCTGAGG CCTCAGC(-5/-2)
BcgI 1322 GCAGGAAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1342 CTTAAG C^TTAAG
BseRI 34 GAGGAG GAGGAG(10/8)
BsiHKAI 697 GAGCAC G[AT]GC[AT]^C
BstAPI 699 GCACGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 890 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1381 GAAGAG CTCTTC(1/4)
Ksp632I 1381 GAAGAG CTCTTC(1/4)
MaeII 317 ACGT A^CGT
MseI 1343 TTAA T^TAA
NotI 856 GCGGCCGC GC^GGCCGC
PvuII 1359 CAGCTG CAG^CTG
SfiI 936 GGCCAAGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 715 CACCGGCG C[GA]^CCGG[CT]G
StuI 761 AGGCCT AGG^CCT
TaqI 443 TCGA T^CGA
Tru9I 1343 TTAA T^TAA
TthHB8I 443 TCGA T^CGA
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# TWO CUT ENZYMES ( 39 enzymes) #
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AccI 385 GTAGAC GT^[AC][GT]AC
1141 GTCTAC
AfaI 372 GTAC GT^AC
1347 GTAC
AlwI 465 GATCC GGATC(4/5)
579 GATCC
AlwNI 130 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
832 CAGGCCCTG
AviII 80 TGCGCA TGC^GCA
497 TGCGCA
BbeI 719 GGCGCC GGCGC^C
1057 GGCGCC
BcnI 360 CCCGG CC^[GC]GG
1283 CCGGG
BmyI 697 GAGCAC G[AGT]GC[ACT]^C
1278 GGGCTC
BsiEI 541 CGGCCG CG[GA][CT]^CG
857 CGGCCG
Bsp1286I 697 GAGCAC G[AGT]GC[ACT]^C
1278 GGGCTC
BsrBI 990 CCGCTC CCGCTC(-3/-3)
598 GAGCGG
BstEII 100 GGTGACC G^GT[ACGT]ACC
970 GGTGACC
BstPI 100 GGTGACC G^GT[ACGT]ACC
970 GGTGACC
BstXI 47 CCATCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1061 CCATGCGGGTGG
BstYI 464 AGATCC [GA]^GATC[CT]
578 AGATCC
DpnII 465 GATC ^GATC
579 GATC
DsaI 20 CCGTGG C^C[GA][CT]GG
146 CCACGG
EagI 541 CGGCCG C^GGCCG
857 CGGCCG
EclXI 541 CGGCCG C^GGCCG
857 CGGCCG
Eco52I 541 CGGCCG C^GGCCG
857 CGGCCG
EcoO65I 100 GGTGACC G^GT[ACGT]ACC
970 GGTGACC
EcoT14I 59 CCAAGG C^C[AT][AT]GG
503 CCAAGG
FspI 80 TGCGCA TGC^GCA
497 TGCGCA
KasI 719 GGCGCC G^GCGCC
1057 GGCGCC
MboI 465 GATC ^GATC
579 GATC
MflI 464 AGATCC [GA]^GATC[CT]
578 AGATCC
NarI 719 GGCGCC GG^CGCC
1057 GGCGCC
NciI 360 CCCGG CC^[GC]GG
1283 CCGGG
NdeII 465 GATC ^GATC
579 GATC
PstI 571 CTGCAG CTGCA^G
1320 CTGCAG
RsaI 372 GTAC GT^AC
1347 GTAC
Sau3AI 465 GATC ^GATC
579 GATC
SbfI 570 CCTGCAGG CCTGCA^GG
1319 CCTGCAGG
SfcI 571 CTGCAG C^T[GA][CT]AG
1320 CTGCAG
SfoI 719 GGCGCC GGC^GCC
1057 GGCGCC
SmlI 802 CTCAAG C^T[CT][GA]AG
1342 CTTAAG
Sse8387I 570 CCTGCAGG CCTGCA^GG
1319 CCTGCAGG
StyI 59 CCAAGG C^C[AT][AT]GG
503 CCAAGG
XhoII 464 AGATCC [GA]^GATC[CT]
578 AGATCC
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BalI BanI BbvI BglI BpmI
BsaHI BsaI BsaJI BsiYI BslI
BsmAI BsmFI BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
EaeI EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeIII MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NgoMIV NlaIII NlaIV PleI PpuMI
Sau96I ScrFI SfaNI TseI Tsp45I
TspRI XcmI