Restriction map for
pF1KB8843
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AhdI Alw44I ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvrII BalI BamHI BbrPI BciVI
BclI BfrI BglII BlnI BsaBI
BseAI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII Eam1105I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI HindIII HpaI KpnI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 863 GACGTC GACGT^C
AccI 845 GTCGAC GT^[AC][GT]AC
AgeI 841 ACCGGT A^CCGGT
AlwNI 991 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 806 AGCGCT AGC^GCT
ApaI 140 GGGCCC GGGCC^C
AspI 848 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 105 TGCGCA TGC^GCA
BbsI 462 GAAGAC GAAGAC(2/6)
BbvCI 984 GCTGAGG CCTCAGC(-5/-2)
BcgI 372 CGAGACCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1179 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1179 GCTAAGC GC^T[ACGT]AGC
BpuAI 462 GAAGAC GAAGAC(2/6)
BsaAI 88 TACGTG [CT]AC^GT[GA]
BsaWI 841 ACCGGT [AT]^CCGG[AT]
BsgI 540 CTGCAC GTGCAG(16/14)
BsmBI 1407 GAGACG CGTCTC(1/5)
BstXI 383 CCACACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1433 AGATCC [GA]^GATC[CT]
CelII 1179 GCTAAGC GC^T[ACGT]AGC
DrdI 307 GACCCCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1261 CGGCCG [CT]^GGCC[GA]
EagI 1261 CGGCCG C^GGCCG
EclXI 1261 CGGCCG C^GGCCG
Eco47III 806 AGCGCT AGC^GCT
Eco52I 1261 CGGCCG C^GGCCG
FspI 105 TGCGCA TGC^GCA
MflI 1433 AGATCC [GA]^GATC[CT]
NaeI 1120 GCCGGC GCC^GGC
NcoI 898 CCATGG C^CATGG
NgoMIV 1120 GCCGGC G^CCGGC
PflFI 848 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 348 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 841 ACCGGT A^CCGGT
PstI 565 CTGCAG CTGCA^G
SalI 845 GTCGAC G^TCGAC
SapI 22 GCTCTTC GCTCTTC(1/4)
SfaNI 103 GATGC GCATC(5/9)
SfcI 565 CTGCAG C^T[GA][CT]AG
SmaI 531 CCCGGG CCC^GGG
Tth111I 848 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 348 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1433 AGATCC [GA]^GATC[CT]
XmaCI 531 CCCGGG C^CCGGG
XmaI 531 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 643 GAATTC [GA]^AATT[CT]
820 GAATTC
AfaI 178 GTAC GT^AC
413 GTAC
AlwI 1434 GATCC GGATC(4/5)
800 GGATC
AocI 449 CCTCAGG CC^T[ACGT]AGG
883 CCTGAGG
ApoI 643 GAATTC [GA]^AATT[CT]
820 GAATTC
AvaI 531 CCCGGG C^[CT]CG[GA]G
1224 CTCGGG
BsaI 373 GAGACC GGTCTC(1/5)
792 GAGACC
BsiEI 843 CGGTCG CG[GA][CT]^CG
1261 CGGCCG
BsoBI 531 CCCGGG C^[CT]CG[GA]G
1224 CTCGGG
BsrBI 688 CCGCTC CCGCTC(-3/-3)
1213 GAGCGG
BsrFI 841 ACCGGT [GA]^CCGG[CT]
1120 GCCGGC
Bsu36I 449 CCTCAGG CC^T[ACGT]AGG
883 CCTGAGG
Cfr10I 841 ACCGGT [GA]^CCGG[CT]
1120 GCCGGC
Eco81I 449 CCTCAGG CC^T[ACGT]AGG
883 CCTGAGG
EcoRI 643 GAATTC G^AATTC
820 GAATTC
EcoT14I 407 CCAAGG C^C[AT][AT]GG
898 CCATGG
HgaI 394 GACGC GACGC(5/10)
1409 GACGC
HincII 845 GTCGAC GT[CT]^[GA]AC
1104 GTTGAC
HindII 845 GTCGAC GT[CT]^[GA]AC
1104 GTTGAC
KspI 272 CCGCGG CCGC^GG
360 CCGCGG
MaeIII 866 GTCAC ^GT[ACGT]AC
913 GTGAC
RsaI 178 GTAC GT^AC
413 GTAC
SacII 272 CCGCGG CCGC^GG
360 CCGCGG
StyI 407 CCAAGG C^C[AT][AT]GG
898 CCATGG
TaqI 803 TCGA T^CGA
846 TCGA
Tsp45I 866 GTCAC ^GT[GC]AC
913 GTGAC
TthHB8I 803 TCGA T^CGA
846 TCGA
XcmI 1391 CCAAGCGCAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1455 CCAGAGTCTAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AluI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI PpuMI Sau3AI
Sau96I ScrFI SfoI TseI Tsp509I
TspRI