##################################
# NO CUT ENZYMES (  217 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        AluI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        Asp718I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BbvI        BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BmyI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        BsoBI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstYI       BstZ17I     Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      ClaI        
CpoI        DdeI        DraI        DraII       DraIII      
DsaI        EaeI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        Fnu4HI      FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HindIII     HpaI        
HpaII       ItaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
TseI        Tsp45I      TspRI       Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AfaI           63  GTAC                 GT^AC
AlwI           37  GATCC                GGATC(4/5)
AlwNI          52  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AseI          177  ATTAAT               AT^TAAT
AsnI          177  ATTAAT               AT^TAAT
Asp700I        90  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         183  GGACC                G^G[AT]CC
BpmI          157  CTCCAG               CTGGAG(16/14)
BstAPI         51  GCAGTTTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I         69  GGATG                GGATG(2/0)
BstXI          77  CCAATGCCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I        183  GGACC                G^G[ACGT]CC
DpnII          37  GATC                 ^GATC
DrdI          118  GACACTCACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI           69  GGATG                GGATG(9/13)
HincII          4  GTCAAC               GT[CT]^[GA]AC
HindII          4  GTCAAC               GT[CT]^[GA]AC
HinfI          73  GAGTC                G^A[ACGT]TC
HphI          172  GGTGA                GGTGA(8/7)
MboI           37  GATC                 ^GATC
MboII          90  GAAGA                GAAGA(8/7)
MnlI          156  CCTC                 CCTC(7/6)
MwoI           51  GCAGTTTCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeII          37  GATC                 ^GATC
NlaIII         45  CATG                 CATG^
PleI           73  GAGTC                GAGTC(4/5)
PshBI         177  ATTAAT               AT^TAAT
RsaI           63  GTAC                 GT^AC
Sau3AI         37  GATC                 ^GATC
Sau96I        183  GGACC                G^G[ACGT]CC
XmnI           90  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BsaJI          83  CCCTGG               C^C[ACGT][ACGT]GG
               84  CCTGGG              
BssKI          84  CCTGG                ^CC[ACGT]GG
              159  CCAGG               
BstNI          84  CCTGG                CC^[AT]GG
              159  CCAGG               
EcoRII         84  CCTGG                ^CC[AT]GG
              159  CCAGG               
MaeII           8  ACGT                 A^CGT
              125  ACGT                
MseI          149  TTAA                 T^TAA
              178  TTAA                
MvaI           84  CCTGG                CC^[AT]GG
              159  CCAGG               
ScrFI          84  CCTGG                CC^[ACGT]GG
              159  CCAGG               
Tru9I         149  TTAA                 T^TAA
              178  TTAA                
Tsp509I       143  AATT                 ^AATT
              176  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################