##################################
# NO CUT ENZYMES (  237 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        AluI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      ClaI        CpoI        
DdeI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoT14I     
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MspI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      Sau96I      SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tru9I       
Tsp45I      TspRI       Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   12 enzymes) #
###################################

BbvI          211  GCAGC                GCAGC(8/12)
BspMI         127  GCAGGT               ACCTGC(4/8)
BsrDI         104  GCAATG               GCAATG(2/0)
Fnu4HI        211  GCAGC                GC^[ACGT]GC
HphI          154  GGTGA                GGTGA(8/7)
ItaI          211  GCAGC                GC^[ACGT]GC
MnlI           86  CCTC                 CCTC(7/6)
NdeI           20  CATATG               CA^TATG
NlaIII         70  CATG                 CATG^
SfaNI         163  GATGC                GCATC(5/9)
SfcI          179  CTACAG               C^T[GA][CT]AG
TseI          211  GCAGC                G^C[AT]GC


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BsaJI         200  CCCAGG               C^C[ACGT][ACGT]GG
              201  CCAGGG              
BssKI          78  CCTGG                ^CC[ACGT]GG
              201  CCAGG               
BstNI          78  CCTGG                CC^[AT]GG
              201  CCAGG               
EcoRII         78  CCTGG                ^CC[AT]GG
              201  CCAGG               
EcoRV          73  GATATC               GAT^ATC
              136  GATATC              
MvaI           78  CCTGG                CC^[AT]GG
              201  CCAGG               
ScrFI          78  CCTGG                CC^[ACGT]GG
              201  CCAGG               
Tsp509I       110  AATT                 ^AATT
              122  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    1 enzymes) #
#########################################

MboII