Restriction map for
pF1KB8862
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# NO CUT ENZYMES ( 209 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRII EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeIII
HgaI Hin1I HincII HindII HindIII
HinfI HpaI HphI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspA1I MunI MvaI
MvnI NaeI NarI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I TspRI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AflIII 44 ACATGT A^C[GA][CT]GT
AluI 15 AGCT AG^CT
AvaII 23 GGTCC G^G[AT]CC
BcnI 290 CCGGG CC^[GC]GG
BmyI 150 GGGCAC G[AGT]GC[ACT]^C
BsmAI 239 GAGAC GTCTC(1/5)
Bsp1286I 150 GGGCAC G[AGT]GC[ACT]^C
BspLU11I 44 ACATGT A^CATGT
BssKI 290 CCGGG ^CC[ACGT]GG
Cac8I 92 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 280 GCGC GCG^C
Cfr13I 23 GGTCC G^G[ACGT]CC
DpnII 39 GATC ^GATC
DsaI 270 CCATGG C^C[GA][CT]GG
EcoT14I 270 CCATGG C^C[AT][AT]GG
HaeII 279 AGCGCC [GA]GCGC^[CT]
HapII 290 CCGG C^CGG
HhaI 280 GCGC GCG^C
HinP1I 280 GCGC G^CGC
HpaII 290 CCGG C^CGG
MaeII 66 ACGT A^CGT
MaeIII 140 GTGAC ^GT[ACGT]AC
MboI 39 GATC ^GATC
MboII 204 GAAGA GAAGA(8/7)
MspI 290 CCGG C^CGG
MwoI 87 GCCAAGCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 290 CCGGG CC^[GC]GG
NcoI 270 CCATGG C^CATGG
NdeII 39 GATC ^GATC
NspI 44 ACATGT [GA]CATG^[CT]
Sau3AI 39 GATC ^GATC
Sau96I 23 GGTCC G^G[ACGT]CC
ScrFI 290 CCGGG CC^[ACGT]GG
SmlI 11 CTCAAG C^T[CT][GA]AG
StyI 270 CCATGG C^C[AT][AT]GG
TaqI 41 TCGA T^CGA
Tsp45I 140 GTGAC ^GT[GC]AC
Tsp509I 157 AATT ^AATT
TthHB8I 41 TCGA T^CGA
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# TWO CUT ENZYMES ( 7 enzymes) #
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BbvI 277 GCAGC GCAGC(8/12)
296 GCAGC
BsaJI 270 CCATGG C^C[ACGT][ACGT]GG
290 CCGGGG
BsiYI 283 CCCTGTTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
284 CCTGTTCCGGG
BslI 283 CCCTGTTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
284 CCTGTTCCGGG
Fnu4HI 277 GCAGC GC^[ACGT]GC
296 GCAGC
ItaI 277 GCAGC GC^[ACGT]GC
296 GCAGC
TseI 277 GCAGC G^C[AT]GC
296 GCAGC
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
DdeI MnlI NlaIII