Restriction map for
pF1KB8869
##################################
# NO CUT ENZYMES ( 163 enzymes) #
##################################
AatII AccI AccII AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I AspEI AspI AviII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglI BglII
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DdeI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HphI
KasI KspI MamI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI MwoI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI XcmI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
Acc65I 262 GGTACC G^GTACC
AfaI 263 GTAC GT^AC
AflIII 324 ACATGT A^C[GA][CT]GT
AlwNI 13 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 238 ATTAAT AT^TAAT
AsnI 238 ATTAAT AT^TAAT
Asp718I 262 GGTACC G^GTACC
AspHI 304 GAGCTC G[AT]GC[AT]^C
AvaI 257 CTCGAG C^[CT]CG[GA]G
AvaII 87 GGTCC G^G[AT]CC
AvrII 150 CCTAGG C^CTAGG
BanI 262 GGTACC G^G[CT][GA]CC
BanII 304 GAGCTC G[GA]GC[CT]^C
BbvI 94 GCAGC GCAGC(8/12)
BclI 173 TGATCA T^GATCA
BcnI 186 CCCGG CC^[GC]GG
BfaI 151 CTAG C^TAG
BlnI 150 CCTAGG C^CTAGG
BmyI 304 GAGCTC G[AGT]GC[ACT]^C
BpmI 19 CTGGAG CTGGAG(16/14)
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
BsmI 248 GCATTC GAATGC(1/-1)
BsoBI 257 CTCGAG C^[CT]CG[GA]G
Bsp1286I 304 GAGCTC G[AGT]GC[ACT]^C
BspLU11I 324 ACATGT A^CATGT
BssSI 133 CACGAG CACGAG(-5/-1)
BstF5I 220 CATCC GGATG(2/0)
BstXI 90 CCAGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 87 GGTCC G^G[ACGT]CC
DraIII 112 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 4 GAAGAG CTCTTC(1/4)
EcoT14I 150 CCTAGG C^C[AT][AT]GG
FbaI 173 TGATCA T^GATCA
Fnu4HI 94 GCAGC GC^[ACGT]GC
FokI 220 CATCC GGATG(9/13)
HapII 187 CCGG C^CGG
HpaII 187 CCGG C^CGG
ItaI 94 GCAGC GC^[ACGT]GC
KpnI 262 GGTACC GGTAC^C
Ksp632I 4 GAAGAG CTCTTC(1/4)
MaeI 151 CTAG C^TAG
MaeII 165 ACGT A^CGT
MseI 239 TTAA T^TAA
MslI 171 CATGATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 187 CCGG C^CGG
NciI 186 CCCGG CC^[GC]GG
NlaIV 262 GGTACC GG[ACGT]^[ACGT]CC
NspI 324 ACATGT [GA]CATG^[CT]
PaeR7I 257 CTCGAG C^TCGAG
PleI 255 GACTC GAGTC(4/5)
PshBI 238 ATTAAT AT^TAAT
RsaI 263 GTAC GT^AC
SacI 304 GAGCTC GAGCT^C
Sau96I 87 GGTCC G^G[ACGT]CC
SmlI 257 CTCGAG C^T[CT][GA]AG
StuI 294 AGGCCT AGG^CCT
StyI 150 CCTAGG C^C[AT][AT]GG
TaqI 258 TCGA T^CGA
TfiI 388 GAATC G^A[AT]TC
Tru9I 239 TTAA T^TAA
TseI 94 GCAGC G^C[AT]GC
Tsp45I 118 GTGAC ^GT[GC]AC
TspRI 112 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 258 TCGA T^CGA
XhoI 257 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 156 AAATTC [GA]^AATT[CT]
196 GAATTT
AlwI 9 GGATC GGATC(4/5)
366 GGATC
ApoI 156 AAATTC [GA]^AATT[CT]
196 GAATTT
BsaJI 77 CCCTGG C^C[ACGT][ACGT]GG
150 CCTAGG
BsiYI 78 CCTGGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
186 CCCGGAAGTGG
BslI 78 CCTGGAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
186 CCCGGAAGTGG
BspHI 176 TCATGA T^CATGA
308 TCATGA
BstNI 78 CCTGG CC^[AT]GG
90 CCAGG
Cac8I 346 GCCAGC GC[ACGT]^[ACGT]GC
376 GCAAGC
EcoRII 78 CCTGG ^CC[AT]GG
90 CCAGG
HaeIII 295 GGCC GG^CC
345 GGCC
HinfI 255 GACTC G^A[ACGT]TC
388 GAATC
MspA1I 95 CAGCTG C[AC]G^C[GT]G
272 CAGCTG
MvaI 78 CCTGG CC^[AT]GG
90 CCAGG
PvuII 95 CAGCTG CAG^CTG
272 CAGCTG
RcaI 176 TCATGA T^CATGA
308 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BbsI BpuAI BssKI DpnII
MaeIII MboI MboII MnlI NdeII
NlaIII Sau3AI ScrFI Tsp509I