Restriction map for
pF1KB8876
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# NO CUT ENZYMES ( 171 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbsI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MluI MluNI
MroI MscI MseI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PleI PmeI PpuMI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tth111I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 241 GTCTAC GT^[AC][GT]AC
AciI 20 GCGG CCGC(-3/-1)
AgeI 172 ACCGGT A^CCGGT
AlwNI 229 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
BanII 193 GGGCTC G[GA]GC[CT]^C
BbrPI 325 CACGTG CAC^GTG
BbvCI 486 CCTCAGC CCTCAGC(-5/-2)
BcnI 312 CCGGG CC^[GC]GG
BsaAI 325 CACGTG [CT]AC^GT[GA]
BsaWI 172 ACCGGT [AT]^CCGG[AT]
BseRI 153 CTCCTC GAGGAG(10/8)
BsrFI 172 ACCGGT [GA]^CCGG[CT]
BstAPI 462 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 172 ACCGGT [GA]^CCGG[CT]
EcoT14I 380 CCTTGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
HindIII 407 AAGCTT A^AGCTT
HinfI 23 GATTC G^A[ACGT]TC
MfeI 110 CAATTG C^AATTG
MspA1I 478 CAGCTG C[AC]G^C[GT]G
MunI 110 CAATTG C^AATTG
NciI 312 CCGGG CC^[GC]GG
NlaIII 468 CATG CATG^
NsiI 1 ATGCAT ATGCA^T
NspI 467 ACATGC [GA]CATG^[CT]
PflMI 228 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 172 ACCGGT A^CCGGT
PmaCI 325 CACGTG CAC^GTG
PmlI 325 CACGTG CAC^GTG
PshAI 108 GACAATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 478 CAGCTG CAG^CTG
StyI 380 CCTTGG C^C[AT][AT]GG
TfiI 23 GATTC G^A[AT]TC
Tsp509I 111 AATT ^AATT
Van91I 228 CCAGACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 422 CCACAGAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AvaII 225 GGACC G^G[AT]CC
483 GGACC
BmyI 64 GTGCCC G[AGT]GC[ACT]^C
193 GGGCTC
BpmI 301 CTGGAG CTGGAG(16/14)
373 CTGGAG
BsaI 376 GAGACC GGTCTC(1/5)
455 GGTCTC
BsiYI 228 CCAGACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
437 CCCTGAGCAGG
BslI 228 CCAGACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
437 CCCTGAGCAGG
Bsp1286I 64 GTGCCC G[AGT]GC[ACT]^C
193 GGGCTC
BstF5I 69 CATCC GGATG(2/0)
204 CATCC
BstYI 251 AGATCC [GA]^GATC[CT]
315 GGATCT
Cac8I 330 GCTGGC GC[ACGT]^[ACGT]GC
443 GCAGGC
FokI 69 CATCC GGATG(9/13)
204 CATCC
HapII 173 CCGG C^CGG
312 CCGG
HpaII 173 CCGG C^CGG
312 CCGG
MaeII 278 ACGT A^CGT
326 ACGT
MaeIII 115 GTCAC ^GT[ACGT]AC
169 GTCAC
MboII 318 TCTTC GAAGA(8/7)
321 TCTTC
MflI 251 AGATCC [GA]^GATC[CT]
315 GGATCT
MslI 258 CACCAGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
423 CACAGAGGTG
MspI 173 CCGG C^CGG
312 CCGG
NlaIV 10 GGAACC GG[ACGT]^[ACGT]CC
194 GGCTCC
PstI 448 CTGCAG CTGCA^G
460 CTGCAG
SfcI 448 CTGCAG C^T[GA][CT]AG
460 CTGCAG
Tsp45I 115 GTCAC ^GT[GC]AC
169 GTCAC
TspRI 233 CACTG CA[GC]TG[ACGT][ACGT]^
366 CAGTG
XhoII 251 AGATCC [GA]^GATC[CT]
315 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsmAI
BsrI BssKI BstNI BstXI Cfr13I
DdeI DpnII EcoRII Fnu4HI HaeIII
HphI ItaI MboI MnlI MvaI
MwoI NdeII Sau3AI Sau96I ScrFI
TseI