################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AfaI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BalI BamHI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrFI BsrGI Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI Ksp632I MamI MboI MfeI MflI MluI MluNI MscI MunI NaeI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SalI SapI Sau3AI SbfI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 59 enzymes) # ################################### AccIII 149 TCCGGA T^CCGGA AcyI 237 GGCGCC G[GA]^CG[CT]C Alw44I 410 GTGCAC G^TGCAC AlwNI 86 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 410 GTGCAC G^TGCAC AspI 311 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 128 TGCGCA TGC^GCA BbeI 237 GGCGCC GGCGC^C BbsI 308 GAAGAC GAAGAC(2/6) BpmI 107 CTGGAG CTGGAG(16/14) BpuAI 308 GAAGAC GAAGAC(2/6) BsaHI 237 GGCGCC G[GA]^CG[CT]C BsaWI 149 TCCGGA [AT]^CCGG[AT] BseAI 149 TCCGGA T^CCGGA BseRI 273 GAGGAG GAGGAG(10/8) BsgI 455 CTGCAC GTGCAG(16/14) BsmFI 23 GTCCC GGGAC(10/14) BspEI 149 TCCGGA T^CCGGA BspHI 318 TCATGA T^CATGA BspMI 131 GCAGGT ACCTGC(4/8) BsrI 26 CCAGT ACTGG(1/-1) BssHII 159 GCGCGC G^CGCGC BssSI 256 CTCGTG CACGAG(-5/-1) BstAPI 85 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 141 GGTGACC G^GT[ACGT]ACC BstF5I 201 CATCC GGATG(2/0) BstPI 141 GGTGACC G^GT[ACGT]ACC DdeI 271 CTGAG C^T[ACGT]AG DraII 21 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO109I 21 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 141 GGTGACC G^GT[ACGT]ACC FokI 201 CATCC GGATG(9/13) FspI 128 TGCGCA TGC^GCA Hin1I 237 GGCGCC G[GA]^CG[CT]C HinfI 153 GATTC G^A[ACGT]TC KasI 237 GGCGCC G^GCGCC KspI 205 CCGCGG CCGC^GG MaeII 480 ACGT A^CGT MaeIII 142 GTGAC ^GT[ACGT]AC MboII 308 GAAGA GAAGA(8/7) MroI 149 TCCGGA T^CCGGA MseI 37 TTAA T^TAA NarI 237 GGCGCC GG^CGCC PflFI 311 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 21 GGGTCCC [GA]G^G[AT]CC[CT] PstI 31 CTGCAG CTGCA^G RcaI 318 TCATGA T^CATGA SacI 97 GAGCTC GAGCT^C SacII 205 CCGCGG CCGC^GG SexAI 312 ACCAGGT A^CC[AT]GGT SfcI 31 CTGCAG C^T[GA][CT]AG SfoI 237 GGCGCC GGC^GCC SmaI 423 CCCGGG CCC^GGG TfiI 153 GATTC G^A[AT]TC Tru9I 37 TTAA T^TAA Tsp45I 142 GTGAC ^GT[GC]AC Tth111I 311 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 423 CCCGGG C^CCGGG XmaI 423 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 98 AGCT AG^CT 348 AGCT AspHI 97 GAGCTC G[AT]GC[AT]^C 410 GTGCAC AvaI 336 CCCGAG C^[CT]CG[GA]G 423 CCCGGG AvaII 22 GGTCC G^G[AT]CC 511 GGACC BanI 69 GGTGCC G^G[CT][GA]CC 237 GGCGCC BanII 97 GAGCTC G[GA]GC[CT]^C 371 GAGCCC BfaI 286 CTAG C^TAG 305 CTAG BsiHKAI 97 GAGCTC G[AT]GC[AT]^C 410 GTGCAC BsoBI 336 CCCGAG C^[CT]CG[GA]G 423 CCCGGG HgaI 416 GACGC GACGC(5/10) 282 GCGTC MaeI 286 CTAG C^TAG 305 CTAG MslI 319 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 442 CACGCTGGTG NlaIII 123 CATG CATG^ 319 CATG SfaNI 264 GATGC GCATC(5/9) 488 GATGC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AccII AciI BbvI BcnI BglI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DsaI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI