Restriction map for
pF1KB8885
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BseRI BsiEI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I CpoI DdeI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeIII MamI MboII MfeI MluI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmlI SnaBI SphI Sse8387I
SspBI SspI SwaI Tsp45I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 519 TCCGGA T^CCGGA
AlwI 196 GGATC GGATC(4/5)
ApaI 380 GGGCCC GGGCC^C
AspHI 78 GAGCTC G[AT]GC[AT]^C
BalI 125 TGGCCA TGG^CCA
BglI 169 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 332 CTCCAG CTGGAG(16/14)
BsaWI 519 TCCGGA [AT]^CCGG[AT]
BseAI 519 TCCGGA T^CCGGA
BsiHKAI 78 GAGCTC G[AT]GC[AT]^C
BsmFI 371 GGGAC GGGAC(10/14)
BspDI 442 ATCGAT AT^CGAT
BspEI 519 TCCGGA T^CCGGA
BstYI 196 GGATCT [GA]^GATC[CT]
ClaI 442 ATCGAT AT^CGAT
DsaI 83 CCGTGG C^C[GA][CT]GG
EaeI 125 TGGCCA [CT]^GGCC[GA]
EcoT14I 164 CCAAGG C^C[AT][AT]GG
MaeII 10 ACGT A^CGT
MflI 196 GGATCT [GA]^GATC[CT]
MluNI 125 TGGCCA TGG^CCA
MroI 519 TCCGGA T^CCGGA
MscI 125 TGGCCA TGG^CCA
MseI 299 TTAA T^TAA
MspA1I 286 CAGCTG C[AC]G^C[GT]G
PpuMI 354 AGGACCC [GA]G^G[AT]CC[CT]
PstI 289 CTGCAG CTGCA^G
PvuII 286 CAGCTG CAG^CTG
SacI 78 GAGCTC GAGCT^C
SfaNI 61 GATGC GCATC(5/9)
SfcI 289 CTGCAG C^T[GA][CT]AG
SmaI 343 CCCGGG CCC^GGG
SpeI 184 ACTAGT A^CTAGT
StuI 16 AGGCCT AGG^CCT
StyI 164 CCAAGG C^C[AT][AT]GG
Tru9I 299 TTAA T^TAA
XhoII 196 GGATCT [GA]^GATC[CT]
XmaCI 343 CCCGGG C^CCGGG
XmaI 343 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaI 192 CTCGGG C^[CT]CG[GA]G
343 CCCGGG
BanI 40 GGCACC G^G[CT][GA]CC
421 GGTGCC
BcnI 343 CCCGG CC^[GC]GG
344 CCGGG
BsoBI 192 CTCGGG C^[CT]CG[GA]G
343 CCCGGG
BspMI 48 ACCTGC ACCTGC(4/8)
251 ACCTGC
Cac8I 18 GCCTGC GC[ACGT]^[ACGT]GC
273 GCTGGC
DpnII 112 GATC ^GATC
197 GATC
DraII 354 AGGACCC [GA]G^G[ACGT]CC[CT]
379 GGGGCCC
EcoO109I 354 AGGACCC [GA]G^G[ACGT]CC[CT]
379 GGGGCCC
FauI 259 CCCGC CCCGC(4/6)
388 GCGGG
HapII 344 CCGG C^CGG
520 CCGG
HinfI 400 GAATC G^A[ACGT]TC
445 GATTC
HpaII 344 CCGG C^CGG
520 CCGG
HphI 105 GGTGA GGTGA(8/7)
46 TCACC
MboI 112 GATC ^GATC
197 GATC
MspI 344 CCGG C^CGG
520 CCGG
NciI 343 CCCGG CC^[GC]GG
344 CCGGG
NdeII 112 GATC ^GATC
197 GATC
Sau3AI 112 GATC ^GATC
197 GATC
TaqI 13 TCGA T^CGA
443 TCGA
TfiI 400 GAATC G^A[AT]TC
445 GATTC
TthHB8I 13 TCGA T^CGA
443 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BfaI BmyI BsaJI BsgI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I EcoRII Fnu4HI FokI
HaeIII ItaI MaeI MnlI MvaI
MwoI NlaIV Sau96I ScrFI TseI
TspRI